Plant Direct PCR Kit Reference: PD105-02 Plant Direct PCR Kit is suitable for direct amplification of plant leaves, seeds, etc., and can be used for high-throughput screening of plant samples that do not contain polysaccharides and polyphenols. The direct amplification DNA polymerase based on the directed evolution has superior tolerance to PCR inhibitors in plants. Meanwhile, it maintains the high amplification performance, which is suitable for the amplification of DNA fragments within 5 kb. The unique Lysis buffer A in the kit can be used to lyse fresh or frozen plant tissues. It is easy to operate and the lysate can be used as a template for amplification without purification. The system contains protective agents that enable crude samples to be effectively amplified after repeated freezing and thawing. 2 × Plant Direct Master Mix only needs to add primers and templates to perform amplification reaction, thereby reducing pipetting operations and improving detection throughput and reproducibility of results. Amplification products are blunt-ended, which are compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C116/C603).
Bst II Pro DNA Polymerase Large Fragment Reference: P703-01 Bst II Pro DNA Polymerase Large Fragment is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Compared with Bst II DNA Polymerase Large Fragment (Vazyme #P702), Bst II Pro DNA Polymerase Large Fragment combines a new generation of hot start technology to inhibit polymerase activity at temperatures below 50°C and release polymerase activity at temperatures above 50°C. It displays superior amplification speed, specificity, salt tolerance and thermal stability. The reaction system can be prepared at room temperature.
Bst II Pro DNA Polymerase Large Fragment Reference: P703-02 Bst II Pro DNA Polymerase Large Fragment is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Compared with Bst II DNA Polymerase Large Fragment (Vazyme #P702), Bst II Pro DNA Polymerase Large Fragment combines a new generation of hot start technology to inhibit polymerase activity at temperatures below 50°C and release polymerase activity at temperatures above 50°C. It displays superior amplification speed, specificity, salt tolerance and thermal stability. The reaction system can be prepared at room temperature.
Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) Reference: PL703-01 Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) combines a new generation of hot start technology to inhibit polymerase activity at temperatures below 50°C and release polymeraseactivity at temperatures above 50°C. It displays good amplification speed, high specificity, strong salt tolerance and reliable thermal stability. The reaction system can be prepared at room temperature. This product is a glycerin-free formula, which can be used to develop lyophilized products. It should be noted that excipient ingredients are not included, please add it according to actual requirements.
Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) Reference: PL703-02 Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) combines a new generation of hot start technology to inhibit polymerase activity at temperatures below 50°C and release polymeraseactivity at temperatures above 50°C. It displays good amplification speed, high specificity, strong salt tolerance and reliable thermal stability. The reaction system can be prepared at room temperature. This product is a glycerin-free formula, which can be used to develop lyophilized products. It should be noted that excipient ingredients are not included, please add it according to actual requirements.
Taq HS DNA Polymerase Reference: P132-d1 Taq HS DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Taq HS DNA polymerase is still blocked at temperature up to 55°C, which minimizes non-specific amplification during the mixing and system heating. When the reaction is kept at 95°C for more than 30 sec, Champagne Taq antibody is completely inactivated and Taq enzyme activity is completely released, ensuring that the PCR system has extremely high amplification sensitivity and specificity. The activation of Taq HS DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR based on Taq DNA polymerase and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). It is the hot-start Taq enzyme of choice for PCR/qPCR molecular diagnostic reagents. Taq HS DNA polymerase has higher stability and detection rate.
Taq HS DNA Polymerase Reference: P132-d2 Taq HS DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Taq HS DNA polymerase is still blocked at temperature up to 55°C, which minimizes non-specific amplification during the mixing and system heating. When the reaction is kept at 95°C for more than 30 sec, Champagne Taq antibody is completely inactivated and Taq enzyme activity is completely released, ensuring that the PCR system has extremely high amplification sensitivity and specificity. The activation of Taq HS DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR based on Taq DNA polymerase and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). It is the hot-start Taq enzyme of choice for PCR/qPCR molecular diagnostic reagents. Taq HS DNA polymerase has higher stability and detection rate.