Category: Molecular Biology Reagents

Reference: C115-02

ClonExpress® Ultra One Step Cloning Kit is a new generation of recombinant cloning kit, which can quickly and efficiently clone 1-5 fragments to any position of any vector. The highly optimized 2×ClonExpress Mix, 50℃ reaction 5-15 Min completes the reorganization, and the operation is simple and quick.Application: Fast: 5-15min rapid reorganization; Simple: One-tube Mix, easy to operate; High efficiency: Highly efficient cloning of 50 bp～10 kb fragments, with a positive rate of >95%; Compatible: single fragment, multi-fragment, entry clone three in one; Zero background: provide zero background, ampicillin/canal double resistance carrier

Reference: C214-01

Mut Express® II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, the target plasmid amplification product is digested with DpnI, ClonExpress® recombination and circularized, and then directly transformed to complete the site-directed mutation. The kit consists of Phanta® Max Super-Fidelity DNA Polymerase amplification module and ClonExpress® rapid cloning module. The ultra-high fidelity of Phanta® MaxSuper-Fidelity DNA Polymerase significantly reduces the possibility of introducing new mutations during the amplification process. The ClonExpress® rapid cloning system uses efficient homologous recombination to replace the traditional annealing loop reaction. Highly optimized reaction buffers, fast operating procedures and extremely high success rate make Mut Express® II Fast Mutagenesis Kit V2 the first choice for DNA site-directed mutagenesis.Application: Phanta® Max Super-Fidelity DNA Polymerase high-fidelity enzyme provides PCR reactions with ultra-high fidelity; The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template; The amplified product can be directly used in the recombination reaction after being digested by DpnI; Site-directed mutagenesis can be performed on a single site or two discrete sites (more than 50 bp apart) on the target plasmid at the same time

Reference: C214-02

Mut Express® II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, the target plasmid amplification product is digested by DpnI, ClonExpress® recombination and circularized, and then directly transformed to complete the site-directed mutation. The kit consists of Phanta® Max Super-Fidelity DNA Polymerase amplification module and ClonExpress® rapid cloning module. The ultra-high fidelity of Phanta® MaxSuper-Fidelity DNA Polymerase significantly reduces the possibility of introducing new mutations during the amplification process. The ClonExpress® rapid cloning system uses efficient homologous recombination to replace the traditional annealing loop reaction. Highly optimized reaction buffers, fast operating procedures and extremely high success rate make Mut Express® II Fast Mutagenesis Kit V2 the first choice for DNA site-directed mutagenesis.Application: Phanta® Max Super-Fidelity DNA Polymerase high-fidelity enzyme provides PCR reactions with ultra-high fidelity; The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template; The amplified product can be directly used in the recombination reaction after being digested by DpnI; Site-directed mutagenesis can be performed on a single site or two discrete sites (more than 50 bp apart) on the target plasmid at the same time

Reference: C215-01

Mut Express® MultiS Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, three to five discrete sites on the target plasmid can be introduced at one time. The kit consists of two The ultra-high fidelity of Phanta® Max significantly reduces the possibility of introducing new mutations during the amplification process. Its excellent long-fragment amplification capability is widely applicable to the amplification of any plasmid with a length less than 20 kb. ClonExpress® rapid cloning System utilizes efficient homologous recombination. The reaction replaces the traditional annealing reaction. Therefore, use Mut Express® MultiS site-directed mutagenesis kit for DNA site-directed In the case of mutation, the primer design is more flexible, and the amplification reaction is no longer performed in a linear manner, and the template usage is extremely low, which is beneficial to the original Complete degradation of the basic template. The kit is equipped with Exnase® MultiS, a recombinase optimized for multi-base site-directed mutagenesis, And if the amplified product is specific, the DpnI digested product can be used directly in the recombination reaction without DNA purification. Highly optimized reaction buffer Mut Express® MuiltiS Fast Mutagenesis Kit V2 has become the preferred kit for DNA multipoint mutations. Application: Phanta® Max Super-Fidelity DNA Polymerase provides high-fidelity PCR with the lowest mutation rate; Phanta® Max Super-Fidelity DNA Polymerase has excellent long fragment amplification ability, which is widely applicable to the amplification of any plasmid within 20 kb; The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template; ClonExpress® rapid cloning system efficiently circularizes PCR products; DpnI eliminates original template contamination Site-directed mutagenesis of three to five discrete sites (more than 50 bp apart) on the target plasmid can be achieved in one step

Reference: C215-02

Mut Express® MultiS Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, three to five discrete sites on the target plasmid can be introduced at one time. The kit consists of two The ultra-high fidelity of Phanta® Max significantly reduces the possibility of introducing new mutations during the amplification process. Its excellent long-fragment amplification capability is widely applicable to the amplification of any plasmid with a length less than 20 kb. ClonExpress® rapid cloning System utilizes efficient homologous recombination. The reaction replaces the traditional annealing reaction. Therefore, use Mut Express® MultiS site-directed mutagenesis kit for DNA site-directed In the case of mutation, the primer design is more flexible, and the amplification reaction is no longer performed in a linear manner, and the template usage is extremely low, which is beneficial to the original. Complete degradation of the basic template. The kit is equipped with Exnase® MultiS, a recombinase optimized for multi-base site-directed mutagenesis, And if the amplified product is specific, the DpnI digested product can be used directly in the recombination reaction without DNA purification. Highly optimized reaction buffer Mut Express® MuiltiS Fast Mutagenesis Kit V2 has become the preferred kit for DNA multipoint mutations. Application: Phanta® Max Super-Fidelity DNA Polymerase provides high-fidelity PCR with the lowest mutation rate; Phanta® Max Super-Fidelity DNA Polymerase has excellent long fragment amplification ability, which is widely applicable to the amplification of any plasmid within 20 kb; The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template; ClonExpress® rapid cloning system efficiently circularizes PCR products; DpnI eliminates original template contamination Site-directed mutagenesis of three to five discrete sites (more than 50 bp apart) on the target plasmid can be achieved in one step

Reference: C301-01

T4 DNA Ligase can catalyze the formation of phosphodiester bonds between the 5'phosphate end and 3'hydroxyl end of the adjacent nucleic acid at the blunt or sticky end of dsDNA. It can also catalyze the connection between RNA and ssDNA or RNA strands in the double strand, but it cannot catalyze the entire single Chain nucleotide connection. Suitable for nucleic acid operations such as labeling RNA 3'-end, circularizing RNA and DNA oligonucleotides and cloning cDNA.Application: Purity is greater than 99%, no exogenous nuclease activity remains; The classic buffer composition allows the ligation reaction to proceed efficiently

Reference: C311-01

5minTM Universal Ligation Mix is ​​a ready-to-use 2× premix that mixes enzyme and Buffer. It contains T4 DNA ligase, which catalyzes the formation of adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA to form phosphate diphosphates. Ester bond. This kit is not only suitable for sticky end ligation, but also compatible with DNA ligation with blunt ends and single-base overhanging ends. The optimized connection enhancer and reaction buffer make the reaction more efficient and convenient. The reaction can be quickly connected at 25°C for 5 minutes. The ligation product can directly transform a variety of chemically competent cells.Application: Compatible: compatible with sticky ends, smooth ends, TA connection, Linker or Adapter connection; Fast: 25℃, 5 minutes can be connected quickly; High efficiency: High cloning efficiency.

Reference: C311-02

5minTM Universal Ligation Mix is ​​a ready-to-use 2× premix that mixes enzyme and Buffer. It contains T4 DNA ligase, which catalyzes the formation of adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA to form phosphate diphosphates. Ester bond. This kit is not only suitable for sticky end ligation, but also compatible with DNA ligation with blunt ends and single-base overhanging ends. The optimized connection enhancer and reaction buffer make the reaction more efficient and convenient. The reaction can be quickly connected at 25°C for 5 minutes. The ligation product can directly transform a variety of chemically competent cells.Application: Compatible: compatible with sticky ends, smooth ends, TA connection, Linker or Adapter connection; Fast: 25℃, 5 minutes can be connected quickly; High efficiency: High cloning efficiency.

Reference: C601-01

This product is the second-generation TOPO cloning kit, which contains the second-generation Topoisomerase, a vector containing the suicide gene ccdB, and a blunt-end factor. Using the second-generation Topoisomerase, with the most suitable buffer, it has higher activity and further improves the cloning efficiency. The efficient connection is completed in 5 minutes at room temperature. Using a vector containing the suicide gene ccdB, when the insert is successfully connected to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thus achieving a "Zero" background. The addition of blunt-end factor makes this product compatible with TA cloning and blunt-end cloning.Application: Compatible: Compatible with TA cloning and blunt end cloning at the same time; Fast: add fragments and react for 5 minutes; High efficiency: high cloning efficiency and positive rate>95%.

Reference: C601-02

This product is the second-generation TOPO cloning kit, which contains the second-generation Topoisomerase, a vector containing the suicide gene ccdB, and a blunt-end factor. Using the second-generation Topoisomerase, with the most suitable buffer, it has higher activity and further improves the cloning efficiency. The efficient connection is completed in 5 minutes at room temperature. Using a vector containing the suicide gene ccdB, when the insert is successfully connected to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thus achieving a "Zero" background. The addition of blunt-end factor makes this product compatible with TA cloning and blunt-end cloning.Application: Compatible: Compatible with TA cloning and blunt end cloning at the same time; Fast: add fragments and react for 5 minutes; High efficiency: high cloning efficiency and positive rate>95%.

Reference: C116-01

The ClonExpress technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning.

ClonExpress Ultra One Step Cloning Kit V2 is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.

Reference: C116-02

The ClonExpress technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning.

ClonExpress Ultra One Step Cloning Kit V2 is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.