E.Z.N.A.® Insect DNA Kit Reference: D0926-01 The E.Z.N.A.® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek’s HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.
E.Z.N.A.® Insect DNA Kit Reference: D0926-02 The E.Z.N.A.® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek’s HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.
E-Z 96 Cycle-Pure Kit Reference: D1043-02 The E-Z 96® Cycle Pure Kit procedure allows for the parallel purification of up to 96 PCR samples from multiple amplifications. The E-Z 96® Cycle Pure Kit utilizes multiwell technology for manual or fully automated high-throughput purification. The HiBind® DNA resin selectively adsorbs DNA molecules in an optimized binding buffer (Buffer CP). After a single wash step, pure PCR products are eluted in water or Elution Buffer provided and are ready to use in subsequent applications.
RTv Reverse Transcriptase Reference: RV101-01 RTv Reverse Transcriptase is obtained from the M-MLV Reverse Transcriptase by directed genetic engineering, which has excellent reverse transcription efficiency, specificity, sensitivity and thermal stability. It is applicable for Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP). With the new generation of hot start technology, the reaction system can be prepared at room temperature. Moreover, the enzyme activity is inhibited at temperatures below 45°C, thus improving specificity.
E-Z 96 Plant DNA Kit Reference: D1086-01 E-Z 96® Plant DNA Kits adapted buffer system from Omega Bio-tek's SP Plant DNA system which is particularly useful to process varieties of plants, such as those with unusually high levels of phenolic compounds or polysaccharides as exemplified in cotton, pine and peanut samples. Up to 50 mg of wet tissue or 12 mg dry tissue can be processed in each well in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of the E-Z 96® DNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.
RTv Reverse Transcriptase (Glycerol-free) Reference: RVL101-01 RTv Reverse Transcriptase (Glycerol-free) is obtained from the M-MLV Reverse Transcriptase by directed genetic engineering, which has excellent reverse transcription efficiency, specificity, sensitivity and thermal stability. It is a lyophilizable version of RTv Reverse Transcriptase (Vazyme #RV101), applicable for Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP). With the new generation of hot start technology, the reaction system can be prepared at room temperature. Moreover, the enzyme activity is inhibited at temperatures below 45°C, thus improving specificity.
E-Z 96 Plant DNA Kit Reference: D1086-02 E-Z 96® Plant DNA Kits adapted buffer system from Omega Bio-tek's SP Plant DNA system which is particularly useful to process varieties of plants, such as those with unusually high levels of phenolic compounds or polysaccharides as exemplified in cotton, pine and peanut samples. Up to 50 mg of wet tissue or 12 mg dry tissue can be processed in each well in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of the E-Z 96® DNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.
Fluorescent LAMP/RT-LAMP Kit Reference: RP711-01 Fluorescent LAMP/RT-LAMP Kit is designed to provide a simple one-step solution for loop-mediated isothermal amplification of DNA or RNA targets. The 2 × Fluorescent LAMP/RT-LAMP Master Mix in this kit contains optimized buffer solution, dNTP, Bst II Pro DNA polymerase and RTv reverse transcriptase for fast and efficient LAMP/RT-LAMP detection. Bst II Pro DNA Polymerase and RTv Reverse Transcriptase use the updated hot start technology, which has excellent specificity and supports the preparation of reaction system at room temperature. This kit provides fluorescent dyes to realize real-time fluorescence detection of LAMP/RT-LAMP.
E-Z 96 Plant DNA DS Kit Reference: D1411-00 The E-Z 96® Plant DNA DS Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethylammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB, binding conditions are adjusted, and DNA is purified using an E-Z 96® DNA Plate. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.