HiScript ® II 1st Strand cDNA Synthesis Kit（+gDNA wiper） Reference: R212-01 HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper) is a special kit for cDNA one-strand synthesis that contains a genomic DNA removal step. The HiScript® II Reverse Transcriptase based on the Kit has a significant improvement compared with the previous generation HiScript® Reverse Transcriptase. Thermal stability, template affinity and inhibitor tolerance. The gDNA wiper used in the Kit can quickly remove genomic DNA contamination at 42°C for 2 minutes, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across exons. 10 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo dT23VN included in the kit has a stronger anchoring ability for Poly A+ RNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo dT23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can synthesize full-length cDNA for cloning (up to 20 kb), but also synthesize cDNA with uniform reverse transcription efficiency for each position of qPCRApplication: Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 10 pg-5 μg total RNA, can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.; gDNA wiper Mix can quickly and completely remove genome contamination
E.Z.N.A.® PX Blood RNA Kit Reference: R1057-00 Omega Bio-tek's E.Z.N.A.® PX Blood RNA Kit is designed for isolation of total RNA from blood samples stored in special preserved reagents and Paxgene tubes. The procedure completely removes contaminants and enzyme inhibitors making RNA isolation fast, convenient, and reliable. RNA purified using the E.Z.N.A.® PX RNA Kit method is ready for applications such as RT-PCR.
ORA™ qPCR Probe ROX H Mix Reference: QPP0301 highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe Master mixes are available in three versions – without ROX, with low or high ROX concentration.
HiScript ® II 1st Strand cDNA Synthesis Kit（+gDNA wiper） Reference: R212-02 HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper) is a special kit for cDNA one-strand synthesis that contains a genomic DNA removal step. The HiScript® II Reverse Transcriptase based on the Kit has a significant improvement compared with the previous generation HiScript® Reverse Transcriptase. Thermal stability, template affinity and inhibitor tolerance. The gDNA wiper used in the Kit can quickly remove genomic DNA contamination at 42°C for 2 minutes, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across exons. 10 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo dT23VN included in the kit has a stronger anchoring ability for Poly A+ RNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo dT23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can synthesize full-length cDNA for cloning (up to 20 kb), but also synthesize cDNA with uniform reverse transcription efficiency for each position of qPCRApplication: Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 10 pg-5 μg total RNA, can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.; gDNA wiper Mix can quickly and completely remove genome contamination
E.Z.N.A.® PX Blood RNA Kit Reference: R1057-01 Omega Bio-tek's E.Z.N.A.® PX Blood RNA Kit is designed for isolation of total RNA from blood samples stored in special preserved reagents and Paxgene tubes. The procedure completely removes contaminants and enzyme inhibitors making RNA isolation fast, convenient, and reliable. RNA purified using the E.Z.N.A.® PX RNA Kit method is ready for applications such as RT-PCR.
ORA™ qPCR Probe ROX H Mix Reference: QPP0305 highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe Master mixes are available in three versions – without ROX, with low or high ROX concentration.
HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) Reference: R312-01 HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.Application: Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product; Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns
SQ Total RNA Kit Reference: R3053-05 The E.Z.N.A.® SQ Total RNA Kit is designed for isolating total RNA from animal, tissue and cultured cells. This solution-based system can be easily scaled up and down based on the starting material. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation that are eliminated. RNA purified using the E.Z.N.A.® SQ Total RNA method can be directly used for applications such as RT-PCR, Northern blotting, and other enzymatic reactions.
ORA™ SEE qPCR Probe ROX H Mix Reference: QPP0601 highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. ORA™ SEE qPCR mixes provide an additional advantage of a simplified tracking of the process, as they are colored with an inert blue dye to make samples much better visible during pipetting and handling. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe Master mixes are available in three versions – without ROX, with low or high ROX concentration.
HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) Reference: R312-02 HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.Application: Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product; Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns
E.Z.N.A.® Total RNA Midi Kit Reference: R6664-00 The E.Z.N.A.® Total RNA Midi Kit is designed for RNA isolation from large amounts of sample. The E.Z.N.A.® Total RNA Midi Kit can purify total RNA from up to 1x10^8 cultured cells or 200 mg animal tissues. By using the HiBind® midi column technology, the E.Z.N.A.® Total RNA Midi Kit greatly reduces processing time when compared to traditional methods such as CsCl ultracentrifugation or phenol/chloroform protocols. Purified RNA can be used in downstream applications such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.
ORA™ SEE qPCR Probe ROX H Mix Reference: QPP0605 highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. ORA™ SEE qPCR mixes provide an additional advantage of a simplified tracking of the process, as they are colored with an inert blue dye to make samples much better visible during pipetting and handling. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe Master mixes are available in three versions – without ROX, with low or high ROX concentration.