AceQ U+ Probe Master Mix Reference: Q113-03 AceQ® U+ Probe Master Mix is a special reagent for qPCR using the probe method. The core component, AceTaq® DNA Polymerase, is a chemically modified hot-start DNA polymerase. With the optimal buffer optimized for qPCR, it can effectively inhibit non-specific amplification and significantly improve amplification efficiency. It is suitable for high-sensitivity qPCR reaction. The dUTP/UDG anti-pollution system is introduced into the reagents, which can completely eliminate the influence of amplified product contamination on qPCR. This product is a 2× pre-mixed reagent, which can obtain a good standard curve in a wide quantitative area, accurately quantify and detect the target gene, with good repeatability and high reliability.Application: Excellent amplification sensitivity: Based on the hot-start AceTaq® DNA Polymerase, it provides perfect amplification performance and sensitivity; Reliable quantitative results: the introduction of Heat-labile UDG anti-pollution system can effectively prevent the contamination of PCR products; Robust repeatability: repeat and reliable quantitative results to meet the data requirements of high-level magazines
AceQ® Universal U+ Probe Master Mix V2 Reference: Q513-02 AceQ Universal U+ Probe Master Mix V2 adopts rigorous chemical modification Acetaq, with a carefully optimized buffer system, which can greatly improve the sensitivity of the probe method. AceQ Universal U+ Probe Master Mix V2 adds a dUTP/UDG anti-pollution system to reduce false positives caused by the high sensitivity of the probe method, and to maximize the authenticity of the results. At the same time, AceQ Universal U+ Probe Master Mix V2 also uses a special ROX Passive Reference Dye, which is suitable for all qPCR instruments without the need to adjust the ROX concentration and is easy to use.Application: Excellent amplification sensitivity: The rigorous chemical method of hot-start enzyme Acetaq DNA Polymerase, combined with a carefully optimized buffer system, can detect as low as a single copy template; Excellent linear relationship: It has a good linear relationship in a wide template interval, and the amplification efficiency is as high as 99%; dUTP/UDG anti-pollution system: the introduction of Heat-Labile UDG anti-pollution system, Heat-labile UDG can rapidly degrade U-containing contaminants at room temperature, completely eliminating the impact of amplified product contamination on qPCR reactions; Broad platform applicability: special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different QPCR instruments
AceQ® Universal U+ Probe Master Mix V2 Reference: Q513-03 AceQ Universal U+ Probe Master Mix V2 adopts rigorous chemical modification Acetaq, with a carefully optimized buffer system, which can greatly improve the sensitivity of the probe method. AceQ Universal U+ Probe Master Mix V2 adds a dUTP/UDG anti-pollution system to reduce false positives caused by the high sensitivity of the probe method, and to maximize the authenticity of the results. At the same time, AceQ Universal U+ Probe Master Mix V2 also uses a special ROX Passive Reference Dye, which is suitable for all qPCR instruments without the need to adjust the ROX concentration and is easy to use.Application: Excellent amplification sensitivity: The rigorous chemical method of hot-start enzyme Acetaq DNA Polymerase, combined with a carefully optimized buffer system, can detect as low as a single copy template; Excellent linear relationship: It has a good linear relationship in a wide template interval, and the amplification efficiency is as high as 99%; dUTP/UDG anti-pollution system: the introduction of Heat-Labile UDG anti-pollution system, Heat-labile UDG can rapidly degrade U-containing contaminants at room temperature, completely eliminating the impact of amplified product contamination on qPCR reactions; Broad platform applicability: special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different QPCR instruments
ChamQ Geno-SNP Probe Master Mix Reference: Q811-02 This product realizes the visualization of the sample addition process through the color change reaction after the amplification template is added, which greatly improves the sample addition efficiency. The Champagne TaqTM DNA Polymerase is a new type of antibody hot-start DNA polymerase, which has many advantages such as wide compatibility, strong amplification performance, and high sensitivity. It is equipped with the most suitable Buffer optimized for Realtime-PCR and patent specificity. The promotion factor ExactorTM perfectly balances the contradiction between amplification specificity and amplification efficiency.Application: Reduce pipetting errors: By providing reagents of different colors, the color change effect during pipetting is used to track the pipetting process and reduce pipetting errors. Amplification is highly specific: the new antibody method hot-start enzyme Champagne TaqTM DNA Polymerase, coupled with optimized Buffer and patented specific promotion factor ExactorTM, effectively avoids primer dimers and non-specific amplification. Excellent amplification performance: single-digit copy gene expression can be detected, and target gene positioning is accurate and reliable. Perfect compatibility: ROX premixed version is available, suitable for all mainstream quantitative PCR machines
ChamQ Geno-SNP Probe Master Mix Reference: Q811-03 This product realizes the visualization of the sample addition process through the color change reaction after the amplification template is added, which greatly improves the sample addition efficiency. The Champagne TaqTM DNA Polymerase is a new type of antibody hot-start DNA polymerase, which has many advantages such as wide compatibility, strong amplification performance, and high sensitivity. It is equipped with the most suitable Buffer optimized for Realtime-PCR and patent specificity. The promotion factor ExactorTM perfectly balances the contradiction between amplification specificity and amplification efficiency.Application: Reduce pipetting errors: By providing reagents of different colors, the color change effect during pipetting is used to track the pipetting process and reduce pipetting errors. Amplification is highly specific: the new antibody method hot-start enzyme Champagne TaqTM DNA Polymerase, coupled with optimized Buffer and patented specific promotion factor ExactorTM, effectively avoids primer dimers and non-specific amplification. Excellent amplification performance: single-digit copy gene expression can be detected, and target gene positioning is accurate and reliable. Perfect compatibility: ROX premixed version is available, suitable for all mainstream quantitative PCR machines
HiScript II One Step qRT-PCR SYBR Green Kit Reference: Q221-01 HiScript® II One Step qRT-PCR SYBR® Green Kit uses the SYBR® Green I chimeric fluorescence method and is designed for quantitative PCR detection using RNA as a template (such as RNA viruses). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. Integrating the superior performance of HiScript® II Reverse Transcriptase and Champagne TaqTM DNA Polymerase, with an optimized buffer system, the detection sensitivity of the One Step qRT-PCR SYBR® Green Kit can reach 1 pg of total RNA. The enhancement factor added to the buffer can effectively reduce the formation of primer dimers and improve specificity. The kit is provided in a convenient Master Mix format. 2 × One Step SYBR® Green Mix contains optimized buffer system, dNTP, specific enhancement factor and SYBR® Green I fluorescent dye; One Step SYBR® Green Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM DNA PolymeraseApplication: Excellent specificity: special additive components can minimize the generation of primer dimers, and the quantitative results are more accurate; Ultra-high detection sensitivity: taking into account high amplification efficiency, it can detect as little as 1 pg of total RNA template
HiScript II One Step qRT-PCR Probe Kit Reference: Q222-01 The detection rate of low copy number templates or weak positive samples is still one of the challenges of clinical diagnosis. HiScript® II One Step qRT-PCR Probe Kit contains the best ratio of HiScript® II Reverse Transcriptase and Champagne TaqTM DNA Polymerase, as well as a fully optimized buffer system, whether it is total RNA, in vitro transcription RNA, or viral RNA, HiScript® II The One Step qRT-PCR Probe Kit has extremely high sensitivity and provides stable and reliable amplification performance. Without too much optimization, multiple amplification can be achieved.Application: Ultra-high detection sensitivity: single-digit copies of templates or 0.1 pg total RNA can be detected; Multiple detection: support multiple probe detection
HiScript II U+ One Step qRT-PCR Probe Kit Reference: Q223-01 The dUTP/UDG anti-contamination system is a very effective means to control the contamination of PCR amplification products. In PCR detection reagents using DNA as a template, it has gradually become a mandatory standard. However, the commonly used E. coli UDG It still has high activity at the usual reverse transcription temperature (42-55℃), which will degrade cDNA, which will reduce the sensitivity of One Step RT-PCR/qPCR. HiScript® II U+ One Step qRT-PCR Probe Kit contains an optimized ratio of dUTP/dNTP mix and heat-labile UDG derived from psychrophilic marine bacteria. Heat-labile UDG has high activity at room temperature and can fully degrade U-containing double-stranded DNA during the mixing process of the reaction system. When the reaction system is heated to 50-55°C (the optimal reaction temperature of HiScript® II), Heat-labile UDG is quickly and completely inactivated, maintaining the integrity of the cDNA, and ensuring that the detection sensitivity is not affected. Application: Support multiple probe detection; UDG anti-pollution system: Heat-labile UDG rapidly degrades U-containing double-stranded DNA contaminants at room temperature. Heat-labile UDG is rapidly and completely inactivated at 50-55°C to maintain the integrity of cDNA; Ultra-high detection sensitivity: single-digit copies of templates or 0.1 pg total RNA can be detected Ultra-high amplification specificity: AceTaq® DNA Polymerase based on chemical hot-start, completely blocked enzyme activity before 95°C, and equipped with a patented specific promotion factor Exactor, which makes amplification more specific;
ChamQ Universal SYBR qPCR Master Mix Reference: Q711-01 ChamQ Universal SYBR qPCR Master Mix is a special premix for qPCR reaction using SYBR Green I chimeric fluorescence method. The core component, Champagne Taq DNA Polymerase, is a novel hot-start DNA polymerase modified by antibody method. It has many advantages such as strong specificity, high detection sensitivity. With the optimal Buffer optimized for qPCR and the specific promoter, it is very suitable for qPCR reaction with high specificity and sensitivity. This kit contains special ROX, Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments, and the amplification can be performed with the addition of primers and templates when preparing the reaction system.
ChamQ Universal SYBR qPCR Master Mix Reference: Q711-02 ChamQ Universal SYBR qPCR Master Mix is a special premix for qPCR reaction using SYBR Green I chimeric fluorescence method. The core component, Champagne Taq DNA Polymerase, is a novel hot-start DNA polymerase modified by antibody method. It has many advantages such as strong specificity, high detection sensitivity. With the optimal Buffer optimized for qPCR and the specific promoter, it is very suitable for qPCR reaction with high specificity and sensitivity. This kit contains special ROX, Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments, and the amplification can be performed with the addition of primers and templates when preparing the reaction system.
Taq Pro Multiple Probe qPCR Mix Reference: QN213-01 Taq Pro Multiple Probe qPCR Mix is a master mix for probe qPCR to detect DNA templates (such as DNA viruses). The core component, Taq Pro HS DNA Polymerase, is a new generation hot start DNA polymerase that has been modified based on antibody technique and upgraded to improve template affinity. Equipped with the most suitable buffer optimized for the qPCR system, amplification performance with multiple targets, amplification specificity and sensitivity for detection of low-copy genes and amplification curve shape are significantly improved. It can provide excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes, with good repeatability and high reliability. It has wide compatibility in terms of template type, template GC content, and primer Tm values. And it also has good tolerance to impurities and is suitable for use in a variety of testing scenarios. The product is a 2 × master mix. Only primers, probes and templates need to be added additionally. It is convenient to use and compatible with fast program to reduce test time.
Taq Pro Multiple Probe qPCR Mix Reference: QN213-02 Taq Pro Multiple Probe qPCR Mix is a master mix for probe qPCR to detect DNA templates (such as DNA viruses). The core component, Taq Pro HS DNA Polymerase, is a new generation hot start DNA polymerase that has been modified based on antibody technique and upgraded to improve template affinity. Equipped with the most suitable buffer optimized for the qPCR system, amplification performance with multiple targets, amplification specificity and sensitivity for detection of low-copy genes and amplification curve shape are significantly improved. It can provide excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes, with good repeatability and high reliability. It has wide compatibility in terms of template type, template GC content, and primer Tm values. And it also has good tolerance to impurities and is suitable for use in a variety of testing scenarios. The product is a 2 × master mix. Only primers, probes and templates need to be added additionally. It is convenient to use and compatible with fast program to reduce test time.