Category: Molecular Biology Reagents

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  • Categories: Retrotranscriptases
Reference: RV101-01

RTv Reverse Transcriptase is obtained from the M-MLV Reverse Transcriptase by directed genetic engineering, which has excellent reverse transcription efficiency, specificity, sensitivity and thermal stability. It is applicable for Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP). With the new generation of hot start technology, the reaction system can be prepared at room temperature. Moreover, the enzyme activity is inhibited at temperatures below 45°C, thus improving specificity.

Reference: RVL101-01

RTv Reverse Transcriptase (Glycerol-free) is obtained from the M-MLV Reverse Transcriptase by directed genetic engineering, which has excellent reverse transcription efficiency, specificity, sensitivity and thermal stability. It is a lyophilizable version of RTv Reverse Transcriptase (Vazyme #RV101), applicable for Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP). With the new generation of hot start technology, the reaction system can be prepared at room temperature. Moreover, the enzyme activity is inhibited at temperatures below 45°C, thus improving specificity.

Reference: RP711-01

Fluorescent LAMP/RT-LAMP Kit is designed to provide a simple one-step solution for loop-mediated isothermal amplification of DNA or RNA targets. The 2 × Fluorescent LAMP/RT-LAMP Master Mix in this kit contains optimized buffer solution, dNTP, Bst II Pro DNA polymerase and RTv reverse transcriptase for fast and efficient LAMP/RT-LAMP detection. Bst II Pro DNA Polymerase and RTv Reverse Transcriptase use the updated hot start technology, which has excellent specificity and supports the preparation of reaction system at room temperature. This kit provides fluorescent dyes to realize real-time fluorescence detection of LAMP/RT-LAMP.

Reference: RP711-02

Fluorescent LAMP/RT-LAMP Kit is designed to provide a simple one-step solution for loop-mediated isothermal amplification of DNA or RNA targets. The 2 × Fluorescent LAMP/RT-LAMP Master Mix in this kit contains optimized buffer solution, dNTP, Bst II Pro DNA polymerase and RTv reverse transcriptase for fast and efficient LAMP/RT-LAMP detection. Bst II Pro DNA Polymerase and RTv Reverse Transcriptase use the updated hot start technology, which has excellent specificity and supports the preparation of reaction system at room temperature. This kit provides fluorescent dyes to realize real-time fluorescence detection of LAMP/RT-LAMP.

Reference: MR201-01

miRNA 1st strand cDNA Synthesis Kit (by tailing A) is a kit for microRNA (miRNA) reverse transcription (RT) by tailing A. Poly A polymerase (PAP) adds a Poly(A) tail to the 3' end of miRNA or total RNA, and then universal reverse transcription primers initiate RT reaction with reverse transcriptase. HiScript miRNA Enzyme Mix contains bioengineered reverse transcriptase and PAP. Combined with the optimized buffer, the reverse transcription of non-miRNAs is effectively inhibited, achieving highly specific qPCR results. 2 × miRNA RT Mix and HiScript miRNA Enzyme Mix contains all components required for miRNA tailing and reverse transcription. Two reactions can be performed simultaneously in one tube.