Take5™ 50 bp DNA Ladder Reference: DNL0302 highQu ready to use DNA ladders are mixtures of highly purified plasmid digests and PCR products. The high ladder purity allows for exceptional stability and room temperature storage. Ladders are ready to be directly loaded on agarose gels and are supplied with the loading dye solution for probe DNA. They provide sharp bands for DNA sizing and allow for approximate DNA quantification by comparing the amount of DNA of the band similar in size to your sample band.
10CFU™ Sensitivity Standards for Mycoplasma orale Reference: 102-2003 Contains 3 vials with 10 CFU each and 2 negative controls. For the validation of detection limit and robustness, individual sample material according to EP 2.6.7 with inactivated mycoplasma particles.
HiScript III Reverse Transcriptase Reference: R302-01 HiScript® III Reverse Transcriptase is an upgraded version of HiScript® II Reverse Transcriptase, which can perform highly efficient reverse transcription reactions at 37°C. HiScript® III. Reverse Transcriptase still retains the thermal stability of the second-generation product HiScript® II Reverse Transcriptase. For RNA with complex secondary structure, the reverse transcription temperature can be increased To 50~55℃, avoid the inhibition of cDNA synthesis by RNA complex secondary structure, and can effectively synthesize high-quality cDNA. In addition, this product still has superior continuous synthesis ability and super Strong impurity tolerance. Application: Extensive template compatibility: compatible with various templates such as animals, plants, viruses, etc.; Super impurity tolerance: It has super tolerance to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid); Excellent reverse transcription efficiency: reverse transcription efficiency is higher than that of second-generation products
E-Z 96 Total RNA Kit Reference: R1034-00 The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5 x 10^5 cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilizing HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High-quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.
Take5™ HR DNA Ladder Reference: DNL0402 highQu ready to use DNA ladders are mixtures of highly purified plasmid digests and PCR products. The high ladder purity allows for exceptional stability and room temperature storage. Ladders are ready to be directly loaded on agarose gels and are supplied with the loading dye solution for probe DNA. They provide sharp bands for DNA sizing and allow for approximate DNA quantification by comparing the amount of DNA of the band similar in size to your sample band.
10CFU™ Sensitivity Standards for Mycoplasma gallisepticum Reference: 102-3003 Contains 3 vials with 10 CFU each and 2 negative controls. For the validation of detection limit and robustness, individual sample material according to EP 2.6.7 with inactivated mycoplasma particles.
HiScript II 1st Strand cDNA Synthesis Kit Reference: R211-01 HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation of HiScript® Reverse Transcriptase, HiScript® II has further greatly improved thermal stability and is very suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript® II adds multiple point mutations, which further enhances template affinity and progress, and has higher tolerance to common reverse transcription inhibitors, which greatly improves the ability to synthesize full-length cDNA. HiScript® II 1st Strand cDNA Synthesis Kit is a first-strand cDNA synthesis kit based on HiScript® II Reverse Transcriptase. It contains all the components needed to synthesize high-quality first-strand cDNA. The product is suitable for subsequent PCR, qPCR and other experiments. 2 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo (dT)23VN included in the kit has a stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo (dT)23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can not only synthesize full-length cDNA (up to 20 kb) for cloning, but also efficiently synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR.Application: Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 1 pg-5 μg total RNA, and can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.
E-Z 96 Total RNA Kit Reference: R1034-01 The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5 x 10^5 cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilizing HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High-quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.
StainIN™ RED Nucleic Acid Stain Reference: NAS0101 StainIN™ RED Nucleic Acid Stain is a significantly safer alternative to ethidium bromide. It is same easy to use, twice as sensitive and much more secure. At least twice as economical as competing products, this novel stain can be also used and disposed with less environmental and health concerns compared to ethidium bromide. StainIN™ RED is a fluorescent dye that allows detection of >0,3 ng of DNA in both agarose and polyacrylamide gels. It binds to both ds DNA, ssDNA and RNA and emits red fluorescence detectable under the UV light and documented with same filters as ethidium bromide. For cloning applications, UV exposure shall be minimized. Much smaller than ethidium bromide carcinogenicity of the dye has been proved by Ames-test. Mammalian cell mutagenicity tests, both mouse marrow erythrocyte micronucleus and spermatocyte chromosomal aberration tests gave negative mutagenicity results.