2 × Phanta® Flash Master Mix(Dye Plus) Reference: P520-02 2 × Phanta Flash Master Mix is based on the new generation superior enzyme Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec/kb) while maintaining high fidelity and yield. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains two types of monoclonal antibodies inhibiting the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. Amplification will generate blunt-ended products, which are compatible with ClonExpress kits (Vazyme #C112/C113/C115) and TOPO cloning kit (Vazyme #C601)Application: This product is suitable for PCR with various templates such as genomic DNA, cDNA, plasmid DNA, dU-containing DNA, crude samples, etc.
2 × Phanta® Flash Master Mix(Dye Plus) Reference: P520-03 2 × Phanta Flash Master Mix is based on the new generation superior enzyme Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec/kb) while maintaining high fidelity and yield. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains two types of monoclonal antibodies inhibiting the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. Amplification will generate blunt-ended products, which are compatible with ClonExpress kits (Vazyme #C112/C113/C115) and TOPO cloning kit (Vazyme #C601)Application: This product is suitable for PCR with various templates such as genomic DNA, cDNA, plasmid DNA, dU-containing DNA, crude samples, etc.
Taq DNA Polymerase (Mg2+ plus Buffer) Reference: P101-01 This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3’ polymerase activity and 5’→3’ exonuclease activity, but no 3’→5’ exonuclease activity. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).Application: Optimized buffer system, convenient to get ideal amplification results quickly
Taq DNA Polymerase (Mg2+ plus Buffer) Reference: P101-02 This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3’ polymerase activity and 5’→3’ exonuclease activity, but no 3’→5’ exonuclease activity. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).Application: Optimized buffer system, convenient to get ideal amplification results quickly
Taq DNA Polymerase (Mg2+ plus Buffer) Reference: P101-03 This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3’ polymerase activity and 5’→3’ exonuclease activity, but no 3’→5’ exonuclease activity. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).Application: Optimized buffer system, convenient to get ideal amplification results quickly
Taq DNA Polymerase (Mg2+ plus Buffer) Reference: P101-d1 This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3’ polymerase activity and 5’→3’ exonuclease activity, but no 3’→5’ exonuclease activity. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).Application: Optimized buffer system, convenient to get ideal amplification results quickly
Taq DNA Polymerase (Mg2+ plus Buffer) Reference: P101-d2 This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3’ polymerase activity and 5’→3’ exonuclease activity, but no 3’→5’ exonuclease activity. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).Application: Optimized buffer system, convenient to get ideal amplification results quickly
Taq DNA Polymerase (Mg2+ plus Buffer) Reference: P101-d3 This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3’ polymerase activity and 5’→3’ exonuclease activity, but no 3’→5’ exonuclease activity. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).Application: Optimized buffer system, convenient to get ideal amplification results quickly
Taq DNA Polymerase (Mg2+ free Buffer) Reference: P102-01 This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3’ polymerase activity and 5’→3’ exonuclease activity, but no 3’→5’ exonuclease activity. The PCR product has an A at the 3'end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).Application: Optimized buffer system, convenient to get ideal amplification results quickly
Taq DNA Polymerase (Mg2+ free Buffer) Reference: P102-02 This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3’ polymerase activity and 5’→3’ exonuclease activity, but no 3’→5’ exonuclease activity. The PCR product has an A at the 3'end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).Application: Optimized buffer system, convenient to get ideal amplification results quickly
Taq DNA Polymerase (Mg2+ free Buffer) Reference: P102-03 This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3’ polymerase activity and 5’→3’ exonuclease activity, but no 3’→5’ exonuclease activity. The PCR product has an A at the 3'end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).Application: Optimized buffer system, convenient to get ideal amplification results quickly
2 × Taq Master Mix Reference: P111-01 This product contains Taq DNA Polymerase, dNTP and an optimized buffer system. It can be amplified by adding primers and templates, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added to the amplification system allows 2 × Master Mix to maintain stable activity after repeated freezing and thawing. This product provides a version containing electrophoresis buffer and dyes, which can be directly electrophoresed after the reaction, which is convenient to use. The PCR product has an A at the 3'end, which can be cloned directly into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113/C114).Application: Optimized buffer system is convenient for obtaining ideal amplification results quickly; Ready-to-use premix, minimizing experimental steps