DNase I , RNase-free Reference: EN402-02 DNase I (deoxyribonuclease I), that is, Deoxyribonuclease I, is an endodeoxyribonuclease that can digest single-stranded or double-stranded DNA. It recognizes and cleaves phosphodiester bonds to produce a 5'-end phosphate group Group, 3'-end hydroxyl monodeoxynucleotide or single-stranded or double-stranded oligodeoxynucleotide. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions Mg2+, Mn2+, etc. It exists in Mg2+ In the case of Mn2+, the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA; and in the presence of Mn2+, it can recognize and cut almost the same site on both strands of DNA, resulting in blunt ends or There are sticky-end DNA fragments with 1-2 nucleotides protruding. This product has undergone strict quality control and is suitable for RNA extraction, in vitro transcription, DNA removal in RT-PCR experiments, DNase I footprinting, nick translatioin, DNA random fragment library construction and other molecular biology experiment. Application: High activity Very low RNase residue Good stability
Bacteria RNA Plus Reagent Reference: R402-01 Bacteria RNA Plus Reagent is an auxiliary reagent optimized for RNA extraction from prokaryotes (including Gram-negative and Gram-positive bacteria). It can be used with Vazyme RNA Isolater Total RNA Extraction Reagent (R401-01) to greatly improve the RNA extraction of prokaryotes. Yield, while ensuring good product quality. Bacteria RNA Plus Reagent contains an optimized buffer system, chelating agents and mild protein denaturants. When extracting bacterial RNA, a high-temperature pretreatment is added before routine operations. Together with Vazyme RNA Isolater Total RNA Extraction Reagent, it can effectively inhibit endogenous RNase promotes protein denaturation, thereby increasing the yield and quality of bacterial RNA. This reagent is also suitable for extracting RNA from yeast and other fungi.Application: Greatly increase the amount of total RNA extracted from bacteria (gram-negative bacteria and gram-positive bacteria). To ensure good quality of RNA products, it is easier to meet the needs of subsequent experiments. The operation is simple, time-saving, no need for lysozyme or ultrasound and other breaking treatments, eliminating the RNA degradation that may be introduced by the operation. No bacterial genomic DNA contamination.
LAMP Fluorescent Dye Reference: RP001-01 LAMP Fluorescent Dye is an intercalating dye with the strong fluorescent signal and little inhibition of the reaction. It is applicable for real-time fluorescent detection of LAMP reactions. This product is a 50 × solution with DMSO as the solvent, compatible with a variety of fluorescent quantitative PCR instruments. It is recommended that the final concentration of the dye be 0.1 - 1×, and please select the SYBR/FAM detection channel when using it.