E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit II Reference: D6950-00 Plasmid isolated with traditional purification procedures normally contains high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. Up to 5 mL of overnight cultures can be used for high copy number plasmids and 10 mL for low copy number. The E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit I integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade(<1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding conditions, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.
E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit II Reference: D6950-01 Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.
E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit II Reference: D6950-02 Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.
HiBind® DNA Mini Columns Reference: DNACOL-01 HiBind® DNA Columns are silica glass fiber columns that can bind up to 100 µg of genomic DNA or 35 µg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation. Omega Bio-tek columns are compatible with the following Qiagen kits: Qiaprep Spin Mini, QiaQuick Gel Extraction, QiaQuick PCR Clean-up, DNeasy Blood & Tissue, DNeasy Plant DNA.
HiBind® DNA Mini Columns Reference: DNACOL-02 HiBind® DNA Columns are silica glass fiber columns that can bind up to 100 µg of genomic DNA or 35 µg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation. Omega Bio-tek columns are compatible with the following Qiagen kits: Qiaprep Spin Mini, QiaQuick Gel Extraction, QiaQuick PCR Clean-up, DNeasy Blood & Tissue, DNeasy Plant DNA.
E-Z 96 Lysate Clearance Plate Reference: FL96-01 The E-Z 96® Lysate Clearance Plate provides a rapid and reliable method to clear insoluble particles during the isolation of plasmid or genomic DNA. Traditional methods use centrifugation which is tedious and inconvenient. The E-Z 96® Lysate Clearance Plate consists of a unique biopolymer-filter membrane in a spin-plate or vacuum-plate format.
E-Z 96 Lysate Clearance Plate Reference: FL96-02 The E-Z 96® Lysate Clearance Plate provides a rapid and reliable method to clear insoluble particles during the isolation of plasmid or genomic DNA. Traditional methods use centrifugation which is tedious and inconvenient. The E-Z 96® Lysate Clearance Plate consists of a unique biopolymer-filter membrane in a spin-plate or vacuum-plate format.
Homogenizer Mini Columns Reference: HCR003 Omega Homogenizer Columns offer a single method to reduce sample viscosity caused by high molecular weight components. Omega Homogenizer columns consist of a unique bipolymer membrane that shreds the lysate's high molecular weight components and removes residual cell debris.