Category: Molecular Biology Reagents

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  • Categories: DNA Extraction
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  • Categories: RNA Extraction
Reference: M1258-00

The Mag-Bind® family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key to the system is Omega Bio-tek's proprietary Mag-Bind® Particle that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer. The Mag-Bind® Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind® technology with the innovative ETR technology (Endo-toxin removal) to deliver high-quality endotoxin-free plasmid DNA in high throughput format. Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 1 mL of overnight culture in LB medium typically produces 10-15 g for high-copy plasmid. The purified plasmid can be used directly for automated fluorescent DNA sequencing, such as with BigDye® sequencing chemistry, transfection as well as for other standard molecular biology techniques including restriction enzyme digestion.

Reference: M1258-01

The Mag-Bind® family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key to the system is Omega Bio-tek's proprietary Mag-Bind® Particle that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer. The Mag-Bind® Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind® technology with the innovative ETR technology (Endo-toxin removal) to deliver high-quality endotoxin-free plasmid DNA in high throughput format. Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 1 mL of overnight culture in LB medium typically produces 10-15 g for high-copy plasmid. The purified plasmid can be used directly for automated fluorescent DNA sequencing, such as with BigDye® sequencing chemistry, transfection as well as for other standard molecular biology techniques including restriction enzyme digestion.

Reference: M1300-05

The Mag-Bind® SeqDTR is designed for efficient and reliable removal of unincorporated dye terminators from sequencing reactions. The system combines Omega Bio-tek's proprietary chemistry with the reversible nucleic acid-binding properties of paramagnetic beads to eliminate excess nucleotides, primers, salts, and unincorporated dye terminators. This kit is designed for both manual and fully automated purification of sequencing products, and the protocol has been successfully automated on liquid handlers such as Beckman Coulter's Biomek® FX/NX, and the Hamilton Microlab® STAR/STARlet. With the high recovery rate from the Mag-Bind® SeqDTR Kit, typically only 1/16 or 1/32 of the ABI Big Dye reagents are needed for sufficient signal strength when compared to sephadex-based clean ups.

Reference: M1300-08

The Mag-Bind® SeqDTR is designed for efficient and reliable removal of unincorporated dye terminators from sequencing reactions. The system combines Omega Bio-tek's proprietary chemistry with the reversible nucleic acid-binding properties of paramagnetic beads to eliminate excess nucleotides, primers, salts, and unincorporated dye terminators. This kit is designed for both manual and fully automated purification of sequencing products, and the protocol has been successfully automated on liquid handlers such as Beckman Coulter's Biomek® FX/NX, and the Hamilton Microlab® STAR/STARlet. With the high recovery rate from the Mag-Bind® SeqDTR Kit, typically only 1/16 or 1/32 of the ABI Big Dye reagents are needed for sufficient signal strength when compared to sephadex-based clean ups.

Reference: M1300-50

The Mag-Bind® SeqDTR is designed for efficient and reliable removal of unincorporated dye terminators from sequencing reactions. The system combines Omega Bio-tek's proprietary chemistry with the reversible nucleic acid-binding properties of paramagnetic beads to eliminate excess nucleotides, primers, salts, and unincorporated dye terminators. This kit is designed for both manual and fully automated purification of sequencing products, and the protocol has been successfully automated on liquid handlers such as Beckman Coulter's Biomek® FX/NX, and the Hamilton Microlab® STAR/STARlet. With the high recovery rate from the Mag-Bind® SeqDTR Kit, typically only 1/16 or 1/32 of the ABI Big Dye reagents are needed for sufficient signal strength when compared to sephadex-based clean ups.

Reference: M1378-00

Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent-to-sample ratio used, giving the user flexibility to perform left, right or double-sided size selection. This product is designed for both manual and fully automated purification of DNA and RNA samples, as well as for the purification of PCR products. The system combines Omega Bio-tek's proprietary chemistries with reversible nucleic acid-binding properties of magnetic beads to selectively bind fragments and eliminate excess nucleotides, primers, and small, non-targeted products such as primer-dimers. Purified DNA and RNA is suitable for a variety of downstream applications such as NGS library preparation, microarrays, automated fluorescent sequencing and restriction enzyme digestion.