Molecular Biology Reagents (21)

Taq HS DNA Polymerase

Reference: P132-d3

Taq HS DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Taq HS DNA polymerase is still blocked at temperature up to 55°C, which minimizes non-specific amplification during the mixing and system heating. When the reaction is kept at 95°C for more than 30 sec, Champagne Taq antibody is completely inactivated and Taq enzyme activity is completely released, ensuring that the PCR system has extremely high amplification sensitivity and specificity. The activation of Taq HS DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR based on Taq DNA polymerase and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). It is the hot-start Taq enzyme of choice for PCR/qPCR molecular diagnostic reagents. Taq HS DNA polymerase has higher stability and detection rate.

miRNA 1st Strand cDNA Synthesis Kit (by tailing A)

Reference: MR201-01

miRNA 1st strand cDNA Synthesis Kit (by tailing A) is a kit for microRNA (miRNA) reverse transcription (RT) by tailing A. Poly A polymerase (PAP) adds a Poly(A) tail to the 3' end of miRNA or total RNA, and then universal reverse transcription primers initiate RT reaction with reverse transcriptase. HiScript miRNA Enzyme Mix contains bioengineered reverse transcriptase and PAP. Combined with the optimized buffer, the reverse transcription of non-miRNAs is effectively inhibited, achieving highly specific qPCR results. 2 × miRNA RT Mix and HiScript miRNA Enzyme Mix contains all components required for miRNA tailing and reverse transcription. Two reactions can be performed simultaneously in one tube.

BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE)

Reference: QN222-01

BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE) is a probe-based qPCR premix for DNA multiplex amplification, which supports premixing of primers and probes. The BioSmart DNA Polymerase is screened by Vazyme's unique BioSmart platform for enzymes with long sequence of shortest extended bases and strong 3' end mismatch recognition ability. With the high blocking rate dual-species specific antibody (binding to different antigenic epitopes) and an optimized buffer system, it effectively improves multiplex amplification sensitivity, specificity, low-copy detection rate, GC content compatibility and impurity tolerance (blood, ethanol, guanidinium salts, etc.). It supports fast program and primers and probes premixing. In addition, the dUTP/UDG anti-contamination system in 2 × All-Powerful qPCR PreMix effectively prevents aerosol contamination, ensuring accurate qPCR results.

BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE)

Reference: QN222-02

BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE) is a probe-based qPCR premix for DNA multiplex amplification, which supports premixing of primers and probes. The BioSmart DNA Polymerase is screened by Vazyme's unique BioSmart platform for enzymes with long sequence of shortest extended bases and strong 3' end mismatch recognition ability. With the high blocking rate dual-species specific antibody (binding to different antigenic epitopes) and an optimized buffer system, it effectively improves multiplex amplification sensitivity, specificity, low-copy detection rate, GC content compatibility and impurity tolerance (blood, ethanol, guanidinium salts, etc.). It supports fast program and primers and probes premixing. In addition, the dUTP/UDG anti-contamination system in 2 × All-Powerful qPCR PreMix effectively prevents aerosol contamination, ensuring accurate qPCR results.

UniPeak U+ One Step RT-qPCR SYBR Green Kit

Reference: Q226-01

UniPeak U+ One Step RT-qPCR SYBR Green Kit is a dye-based RT-qPCR reagent specifically designed for quantitative PCR detection using RNA as the template. The dye-based fluorescence quantitative detection presents two major challenges: the difficulty of detecting low-concentration samples and the issue of non-specific amplification. This product addresses these challenges with a next-generation antibody-modified Taq DNA Polymerase and a one-step, temperature-activated Reverse Transcriptase, combined with an optimized buffer system for RT-qPCR, offering enhanced sensitivity and specificity. To further ensure accurate quantification, a dUTP/UDG anti-contamination system is included, eliminating aerosol contamination at room temperature. Additionally, the 5 × gDNA Wiper effectively removes any residual genomic DNA contamination from RNA templates. The 2 × One Step SYBR Green Master Mix includes tracking dye and universal ROX Reference Dye, making it compatible with all types of qPCR instruments, requiring only the addition of primers and templates for amplification.

FastPure Universal Plant Total RNA Isolation Kit

Reference: RC411-01

FastPure Universal Plant Total RNA Isolation Kit can be used to quickly extract total RNA from plant tissues. The kit includes two solution systems designed for simple RNA extraction from a variety of plant tissues (wheat, rice, corn, Arabidopsis thaliana, tobacco, rape, etc.), polysaccharide and polyphenol rich plant tissues (cotton leaf, soybean leaf, pine needle, ginkgo leaf, fig leaf, gardenia leaf, wheat seed, corn seed, red bean seed, potato, sweet potato, soybean seed, sesame seed, peanut seeds, rapeseed, etc.), fruit pulp (watermelon, apple, peach, pear, banana, mango, etc.), and fungi (shiitake, button mushroom, oyster mushroom, Neurospora crassa, etc.). The kit is based on silica column purification technology and does not involve toxic reagents (e.g., phenol or chloroform and β-mercaptoethanol) or time-consuming alcohol precipitation. The whole extraction process takes only 11 min. FastPure gDNA-Filter Columns III in the kit effectively remove impurities and gDNA. FastPure RNA Columns V efficiently bind to RNA and, paired with optimized buffers, yield high-purity total RNA with minimal gDNA residue and no protein or other impurities that can be used for a range of downstream experiments, including RT-PCR, real-time PCR, RNA library preparation, microarray analysis, Northern blot, dot blot, and molecular cloning.

ClonExpress Ultra One Step Cloning Kit V3

Reference: C117-01

ClonExpress Ultra One Step Cloning Kit V3 utilizes the latest optimized technology, enabling highly efficient homologous recombination of 1 - 11 DNA fragments. The newly optimized 2 × CE Mix V3 not only ensures a high cloning success rate for complicated situations such as large vectors and GC-rich/poor homologous arms, but also significantly enhances compatibility with amounts of DNA input. This greatly solves the problem of reduced cloning efficiency caused by low DNA fragment concentration.

Features

Robust performance: 1 - 11 fragments assembly; the positive rate is > 95%

High Efficiency: 5 - 30 min rapid recombination; high clones and positivity rate

Special optimization: Maximum compatible with 15 - 35 bp overlaps, not only 15 - 20 bp

Broad Compatibility: Suitable for ultra-low input, GC-rich/poor overlaps, and low-purity samples