Nuclear Actin-Chromobody® plasmid (TagGFP2) Reference: acg-n 20 µg nuclear Actin-Chromobody® fused to TagGFP2.
ClonExpress II One Step Cloning Kit Reference: C112-01 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the vector and place the insert 5'in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end, so that the 5'and 3'ends of the PCR product have the same sequence as the two ends of the linearized vector (15 bp-20 bp). This PCR product and the linearized vector are in a certain ratio After mixing, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning. As a new generation recombinant cloning kit, ClonExpress® II has a unique non-ligase dependent system, which greatly reduces the background of vector self-ligation, and the positive rate can reach more than 95%. The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities, making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification, greatly simplifying The experimental steps. Application: Simple, fast, efficient, suitable for any carrier; No need to consider the restriction site carried by the insert; Efficient cloning of 50 bp-10 kb DNA fragments; Linearized cloning vector and PCR products can be cloned directly without purification; No ligase, no self-connection, positive rate >95%
ClonExpress II One Step Cloning Kit Reference: C112-02 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the vector and place the insert 5'in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end, so that the 5'and 3'ends of the PCR product have the same sequence as the two ends of the linearized vector (15 bp-20 bp). This PCR product and the linearized vector are in a certain ratio After mixing, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning.As a new generation recombinant cloning kit, ClonExpress® II has a unique non-ligase dependent system, which greatly reduces the background of vector self-ligation, and the positive rate can reach more than 95%. The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities, making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification, greatly simplifying The experimental steps. Application: Simple, fast, efficient, suitable for any carrier; No need to consider the restriction site carried by the insert; Efficient cloning of 50 bp-10 kb DNA fragments; Linearized cloning vector and PCR products can be cloned directly without purification; No ligase, no self-connection, positive rate >95%
ClonExpress MultiS One Step Cloning Kit Reference: C113-01 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Use any method to linearize the vector, and perform forward/reverse PCR on the insert. The 5'end of the primer is introduced into the end sequence of the linearized vector, so that the 5'and 3'ends of the PCR product have the same sequence (15 bp-20 bp) as the two ends of the linearized vector. After the PCR product of the sequence and the linearized vector are mixed in a certain proportion, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning.As a new generation of recombination cloning kit, ClonExpress® MultiS is specifically optimized for multi-fragment recombination reactions, which can realize sequential splicing cloning of up to five inserts at a time. The unique non-ligase-dependent system of the kit greatly reduces the background of vector self-ligation, and does not need to consider the enzyme cleavage site carried by the insert itself; the highly optimized reaction buffer and the enhanced recombinase Exnase MultiS can significantly improve The recombination efficiency of the clone and the tolerance to impurities make it possible to directly use the linearized vector and insert for recombination cloning without purification, which greatly simplifies the experimental steps. Application: Multi-fragment splicing cloning can be performed at almost any position of any vector; No need to consider the restriction site carried by the insert itself; Sequential splicing of two to five inserts can be realized at one time; Linearized cloning vector and PCR products can be cloned directly without purification
ClonExpress MultiS One Step Cloning Kit Reference: C113-02 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Use any method to linearize the vector, and perform forward/reverse PCR on the insert. The 5'end of the primer is introduced into the end sequence of the linearized vector, so that the 5'and 3'ends of the PCR product have the same sequence (15 bp-20 bp) as the two ends of the linearized vector. After the PCR product of the sequence and the linearized vector are mixed in a certain proportion, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning. As a new generation of recombination cloning kit, ClonExpress® MultiS is specifically optimized for multi-fragment recombination reactions, which can realize sequential splicing cloning of up to five inserts at a time. The unique non-ligase-dependent system of the kit greatly reduces the background of vector self-ligation, and does not need to consider the enzyme cleavage site carried by the insert itself; the highly optimized reaction buffer and the enhanced recombinase Exnase MultiS can significantly improve The recombination efficiency of the clone and the tolerance to impurities make it possible to directly use the linearized vector and insert for recombination cloning without purification, which greatly simplifies the experimental steps. Application: Multi-fragment splicing cloning can be performed at almost any position of any vector; No need to consider the restriction site carried by the insert itself; Sequential splicing of two to five inserts can be realized at one time; Linearized cloning vector and PCR products can be cloned directly without purification
ClonExpress Ultra One Step Cloning Kit Reference: C115-01 ClonExpress® Ultra One Step Cloning Kit is a new generation of recombinant cloning kit, which can quickly and efficiently clone 1-5 fragments to any position of any vector. The highly optimized 2×ClonExpress Mix, 50℃ reaction 5-15 Min completes the reorganization, and the operation is simple and quick.Application: Fast: 5-15min rapid reorganization; Simple: One-tube Mix, easy to operate; High efficiency: Highly efficient cloning of 50 bp～10 kb fragments, with a positive rate of >95%; Compatible: single fragment, multi-fragment, entry clone three in one; Zero background: provide zero background, ampicillin/canal double resistance carrier