miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) Reference: MR101-02 miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) is a special kit suitable for stem-loop miRNA cDNA one-strand synthesis. It contains genomic DNA removal steps, which can quickly remove genomic DNA contamination at 42℃ for 2 min. The follow-up results are more reliable. The HiScript ® II Reverse Transcriptase on which the kit is based has extremely high thermal stability, coupled with an optimized buffer system, which is conducive to the generation of miRNA-specific reverse transcription products. For subsequent quantification of cDNA products, it is recommended to use our company's miRNA Universal SYBR ® qPCR Master Mix (Vazyme # MQ101) to obtain the best experimental results. At the same time, the design software for miRNA reverse transcription stem-loop primers and quantitative primers was launched, which is easy to operate and escort miRNA experiments.Application: Excellent linear relationship: It has a good linear relationship in a wide template interval, and can detect RNA templates as low as pg; Superior reaction system: Optimal Buffer composition and concentration, more suitable for reverse transcription of microRNA; Simple primer design: Provide matching primer design software to make primer design easier
ChamQ Universal SYBR qPCR Master Mix Reference: Q711-02 This product is a special premix for qPCR reaction using SYBR® Green I chimeric fluorescence method. The core component ChampagneTM Taq DNA Polymerase is a new type of antibody-based hot-start DNA polymerase, which has many advantages such as strong specificity and high detection sensitivity. It is equipped with the most suitable buffer optimized for qPCR and the patented specific promoter ExactorTM, which is very suitable For high specificity and high sensitivity qPCR reaction. This product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments. You only need to add primers and templates when preparing the reaction system to perform amplification.Application: Wide platform applicability: special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different instruments; Ultra-high amplification specificity: the use of a new antibody method hot-start enzyme Champagne TaqTM DNA Polymerase, coupled with optimized Buffer and patent specific promotion factor ExactorTM, effectively avoid the generation of primer dimers and non-specific amplification; Excellent amplification sensitivity: The master mix has an excellent linear relationship in a wide template range, and can detect single-digit copies of the template to be tested; Excellent program compatibility: Standard programs and fast programs can be used to quantify the premix, which perfectly solves program compatibility.
ChamQ Universal SYBR qPCR Master Mix Reference: Q711-03 This product is a special premix for qPCR reaction using SYBR® Green I chimeric fluorescence method. The core component ChampagneTM Taq DNA Polymerase is a new type of antibody-based hot-start DNA polymerase, which has many advantages such as strong specificity and high detection sensitivity. It is equipped with the most suitable buffer optimized for qPCR and the patented specific promoter ExactorTM, which is very suitable For high specificity and high sensitivity qPCR reaction. This product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments. You only need to add primers and templates when preparing the reaction system to perform amplification.Application: Wide platform applicability: special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different instruments; Ultra-high amplification specificity: the use of a new antibody method hot-start enzyme Champagne TaqTM DNA Polymerase, coupled with optimized Buffer and patent specific promotion factor ExactorTM, effectively avoid the generation of primer dimers and non-specific amplification; Excellent amplification sensitivity: The master mix has an excellent linear relationship in a wide template range, and can detect single-digit copies of the template to be tested; Excellent program compatibility: Standard programs and fast programs can be used to quantify the premix, which perfectly solves program compatibility.
Taq Pro Universal SYBR qPCR Master Mix Reference: Q712-02 The Taq Pro Universal SYBR qPCR Master Mix is a special master mix for qPCR by SYBR Green I chimeric fluorescence method. The Taq Pro DNA Polymerase used in this product is a new generation hot start polymerase that is upgraded and modified using an antibody method, with the advantages of high specificity, high detection sensitivity and high amplification yield. Combined with the optimal buffer optimized for qPCR and the patented specific promoting factors, it greatly improves the qPCR amplification yield, with a ultra-high plateau value, and is suitable for qPCR amplification with high specificity and sensitivity. Taq Pro Universal SYBR qPCR Master Mix contains a unique ROX Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments.Application: Ultra high plateau value. Perfect balance between sensitivity and specificity. All platform universality
Taq Pro Universal SYBR qPCR Master Mix Reference: Q712-03 The Taq Pro Universal SYBR qPCR Master Mix is a special master mix for qPCR by SYBR Green I chimeric fluorescence method. The Taq Pro DNA Polymerase used in this product is a new generation hot start polymerase that is upgraded and modified using an antibody method, with the advantages of high specificity, high detection sensitivity and high amplification yield. Combined with the optimal buffer optimized for qPCR and the patented specific promoting factors, it greatly improves the qPCR amplification yield, with a ultra-high plateau value, and is suitable for qPCR amplification with high specificity and sensitivity. Taq Pro Universal SYBR qPCR Master Mix contains a unique ROX Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments.Application: Ultra high plateau value. Perfect balance between sensitivity and specificity. All platform universality
AceQ® Universal SYBR qPCR Master Mix Reference: Q511-02 This product is a special reagent for qPCR using the SYBR® Green I chimeric fluorescence method. It uses a chemically modified hot-start DNA polymerase AceTaq® DNA Polymerase, with the optimal buffer optimized for qPCR, which can effectively inhibit non-specific amplification. Thus, the amplification efficiency is significantly improved, and it is suitable for high-sensitivity qPCR reactions. This product is a 2× premixed reagent containing the optimal concentration of SYBR® Green I for qPCR reaction. It can obtain a good standard curve in a wide quantitative area, and accurately quantify and detect target genes with good repeatability and credibility. Degree is high. In addition, the product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments. You only need to add primers and templates when preparing the reaction system to perform amplification.Application: Universal on all platforms: Special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different instruments Rigorous hot-start enzyme: AceTaq DNA Polymerase, based on chemical hot-start, provides perfect amplification specificity; Optimized reaction body: patented buffer formula, which minimizes non-specific amplification and primer dimerization, without repeated optimization of conditions; Excellent amplification performance: repeatable and reliable quantitative results to meet the data requirements of high-level magazines
AceQ® Universal SYBR qPCR Master Mix Reference: Q511-03 This product is a special reagent for qPCR using the SYBR® Green I chimeric fluorescence method. It uses a chemically modified hot-start DNA polymerase AceTaq® DNA Polymerase, with the optimal buffer optimized for qPCR, which can effectively inhibit non-specific amplification. Thus, the amplification efficiency is significantly improved, and it is suitable for high-sensitivity qPCR reactions. This product is a 2× premixed reagent containing the optimal concentration of SYBR® Green I for qPCR reaction. It can obtain a good standard curve in a wide quantitative area, and accurately quantify and detect target genes with good repeatability and credibility. Degree is high. In addition, the product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments. You only need to add primers and templates when preparing the reaction system to perform amplification.Application: Universal for all platforms: special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different instruments Rigorous hot-start enzyme: AceTaq DNA Polymerase, based on chemical hot-start, provides perfect amplification specificity; Optimized reaction body: patented buffer formula, which minimizes non-specific amplification and primer dimerization, without repeated optimization of conditions; Excellent amplification performance: repeatable and reliable quantitative results to meet the data requirements of high-level magazines
AceQ ® qPCR SYBR Green Master Mix Reference: Q111-02 This product is a special reagent for qPCR using SYBR® Green I chimeric fluorescence method. The core component, AceTaq® DNA Polymerase, is a chemically modified hot-start DNA polymerase. With the optimal buffer optimized for qPCR, it can effectively inhibit non-specific amplification and significantly improve amplification efficiency. It is suitable for high-sensitivity qPCR reaction. This product is a 2× premixed reagent containing the optimal concentration of SYBR® Green I for qPCR reaction. It can obtain a good standard curve in a wide quantitative area, and accurately quantify and detect target genes with good repeatability and credibility. Degree is high.Application: Rigorous hot-start enzyme: AceTaq DNA Polymerase, based on chemical hot-start, provides a perfect reaction system optimized for amplification specificity: patented buffer formula, which minimizes non-specific amplification and primer dimerization without repeated optimization of conditions Excellent amplification performance: repeatable and reliable quantitative results to meet the data requirements of high-level magazines
AceQ ® qPCR SYBR Green Master Mix Reference: Q111-03 This product is a special reagent for qPCR using SYBR® Green I chimeric fluorescence method. The core component, AceTaq® DNA Polymerase, is a chemically modified hot-start DNA polymerase. With the optimal buffer optimized for qPCR, it can effectively inhibit non-specific amplification and significantly improve amplification efficiency. It is suitable for high-sensitivity qPCR reaction. This product is a 2× premixed reagent containing the optimal concentration of SYBR® Green I for qPCR reaction. It can obtain a good standard curve in a wide quantitative area, and accurately quantify and detect target genes with good repeatability and credibility. Degree is high.Application: Rigorous hot-start enzyme: AceTaq DNA Polymerase, based on chemical hot-start, provides a perfect reaction system optimized for amplification specificity: patented buffer formula, which minimizes non-specific amplification and primer dimerization without repeated optimization of conditions Excellent amplification performance: repeatable and reliable quantitative results to meet the data requirements of high-level magazines
AceQ ® qPCR Probe Master Mix Reference: Q112-02 This product is a special reagent for qPCR using the probe method. This product is based on the hot-start AceTaq® DNA Polymerase, combined with the optimal buffer optimized for qPCR, can effectively inhibit non-specific amplification, thereby significantly improving amplification efficiency, and is suitable for high-sensitivity qPCR reactions. This product is a 2 × Mix pre-mixed reagent, easy to use. Using this product for qPCR reaction can obtain a good standard curve in a wide quantitative area, accurately quantify and detect the target gene, with good repeatability and high reliability.Application: Excellent amplification sensitivity: Based on the hot-start AceTaq® DNA Polymerase, it provides perfect amplification performance and sensitivity; Robust repeatability: repeat and reliable quantitative results to meet the data requirements of high-level magazines
AceQ ® qPCR Probe Master Mix Reference: Q112-03 This product is a special reagent for qPCR using the probe method. This product is based on the hot-start AceTaq® DNA Polymerase, combined with the optimal buffer optimized for qPCR, can effectively inhibit non-specific amplification, thereby significantly improving amplification efficiency, and is suitable for high-sensitivity qPCR reactions. This product is a 2 × Mix pre-mixed reagent, easy to use. Using this product for qPCR reaction can obtain a good standard curve in a wide quantitative area, accurately quantify and detect the target gene, with good repeatability and high reliability.Application: Excellent amplification sensitivity: Based on the hot-start AceTaq® DNA Polymerase, it provides perfect amplification performance and sensitivity; Robust repeatability: repeat and reliable quantitative results to meet the data requirements of high-level magazines
AceQ U+ Probe Master Mix Reference: Q113-02 AceQ® U+ Probe Master Mix is a special reagent for qPCR using the probe method. The core component, AceTaq® DNA Polymerase, is a chemically modified hot-start DNA polymerase. With the optimal buffer optimized for qPCR, it can effectively inhibit non-specific amplification and significantly improve amplification efficiency. It is suitable for high-sensitivity qPCR reaction. The dUTP/UDG anti-pollution system is introduced into the reagents, which can completely eliminate the influence of amplified product contamination on qPCR. This product is a 2× pre-mixed reagent, which can obtain a good standard curve in a wide quantitative area, accurately quantify and detect the target gene, with good repeatability and high reliability.Application: Excellent amplification sensitivity: Based on the hot-start AceTaq® DNA Polymerase, it provides perfect amplification performance and sensitivity; Reliable quantitative results: the introduction of Heat-labile UDG anti-pollution system can effectively prevent the contamination of PCR products; Robust repeatability: repeat and reliable quantitative results to meet the data requirements of high-level magazines