E.Z.N.A.® RNA-Lock Reagent Reference: R0424-02 The RNA-Lock Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, and white blood cells into the RNA-Lock Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at -20°C.
iSWAB RNA Collection Kit v2, 1.0ml Reference: ISWAB‐RNA‐v2 The iSWAB-RNA-v2 device allows for the non-invasive collection, concentration, and stabilization of intact buccal cells and/or any mammalian cells collected with a swab or cytobrush allowing for real time ambient stabilization of total RNA from the point of collection to processing. Maintaining RNA stability and protecting it from degradation is a significant challenge, while current RNA stabilization methods such as PAXgene, Tempus, RNAgard or dry blood spots require invasive blood collection resulting in low compliance.
qScriber™ cDNA Synthesis Kit Reference: RTK0101 The qScriber™ cDNA Synthesis Kit is a highly efficient and simple-to-use system for cDNA synthesis eliminating the need for tedious reaction optimization. The qScriber™ Enzyme Blend ensures high sensitivity detection from low copy number targets. The highly active and thermostable HighScriber™ Reverse Transcriptase blended with RNase Inhibitor allows for an efficient cDNA synthesis and reaction safety. The wide reaction temperature range (38°C - 55°C) ensures efficient transcription from GC rich templates. The 5X qScriber™ Reaction Mix includes optimal concentrations of magnesium and dNTPs and a combination of anchored oligo (dT) and random hexamers for unbiased representation of mRNA ends. The kit is an optimal choice for generating high quality cDNA from viral RNA, miRNA or other targets for qPCR or for PCR. For the maximum convenience the Kit includes even the PCR Water to set up the reaction, so the only thing you need to take care of is the high quality RNA template.
pSpot7 Reference: ev-7 1.25 µg pSpot7 vector, S.cerevisiae, Spot N-term, Leu/Amp (Ori: 2µ/pBR322), high expression.
E-Z 96 Plant RNA Kit Reference: R1027-01 Based on the same principle of E.Z.N.A.® Plant RNA Kit, the E-Z 96® Plant RNA kit is designed for isolation of plant RNA in a 96-well plate format. The E-Z 96® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. The kit includes a homogenization plate to efficiently remove cell debris and simultaneously homogenize the lysate. In combination with the HiBind RNA plate, this kit permits purification of high-quality RNA from as much as 40 mg seed tissue or up to 100 mg plant tissue. The system is also efficient enough to allow isolation of total RNA from as little as 0.5 mg tissue. Purified RNA is suitable for RT-PCR, Northern analysis, differential display, and poly A+ RNA selection.
iSWAB Protein Collection Kit, 1.0ml Reference: ISWAB‐P‐1200 Non-invasive sample collection tube for collection and stabilization of protein and DNA in the same oral sample. Oral sample collection has gained widespread acceptance due to its non-invasive nature and because continuous visual contact of the collection process is possible. Current non-invasive collection products are designed for immediate detection using stick-based technology, but are limited to simple yes/no answers. If additional data is required, a more robust collection method coupled with high-sensitivity downstream analysis is necessary.
qScriber™ cDNA Synthesis Kit Reference: RTK0104 The qScriber™ cDNA Synthesis Kit is a highly efficient and simple-to-use system for cDNA synthesis eliminating the need for tedious reaction optimization. The qScriber™ Enzyme Blend ensures high sensitivity detection from low copy number targets. The highly active and thermostable HighScriber™ Reverse Transcriptase blended with RNase Inhibitor allows for an efficient cDNA synthesis and reaction safety. The wide reaction temperature range (38°C - 55°C) ensures efficient transcription from GC rich templates. The 5X qScriber™ Reaction Mix includes optimal concentrations of magnesium and dNTPs and a combination of anchored oligo (dT) and random hexamers for unbiased representation of mRNA ends. The kit is an optimal choice for generating high quality cDNA from viral RNA, miRNA or other targets for qPCR or for PCR. For the maximum convenience the Kit includes even the PCR Water to set up the reaction, so the only thing you need to take care of is the high quality RNA template.
M-MLV(H-) Reverse Transcriptase Reference: R021-01 Wild-type M-MLV (Moloney Murine Leukemia Virus) has the following activities: RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity; RNase H activity. Because RNase H activity can catalyze the degradation of RNA in the DNA/RNA hybrid strand, the template RNA may be degraded in the cDNA one-strand synthesis reaction. M-MLV (H-) Reverse Transcriptase is an M-MLV mutant with loss of RNase H activity obtained by site-directed mutagenesis. Compared with the common mutants obtained by deleting the RNase H domain, this product retains the complete protein structure, so it has the same polymerase activity as the wild type, and can be used for longer cDNA synthesis and full-length cDNA library Construction, etc. It also has strand displacement activity and can be used in experiments such as 5'RACE.Application: Point mutations eliminate RNase H activity to obtain long cDNA products; Stable and reliable reverse transcription performance for RNA templates above 100 ng; Synthetic fragment ≤5 kb; Can be used for experiments such as 5’-RACE reaction and cDNA library construction
pSpot6 Reference: ev-6 1.25 µg pSpot6 vector, S.cerevisiae, Spot C-term, Leu/Amp (Ori: CEN/pBR322), low expression.
E-Z 96 Plant RNA Kit Reference: R1027-02 Based on the same principle of E.Z.N.A.® Plant RNA Kit, the E-Z 96® Plant RNA kit is designed for isolation of plant RNA in a 96-well plate format. The E-Z 96® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. The kit includes a homogenization plate to efficiently remove cell debris and simultaneously homogenize the lysate. In combination with the HiBind RNA plate, this kit permits purification of high-quality RNA from as much as 40 mg seed tissue or up to 100 mg plant tissue. The system is also efficient enough to allow isolation of total RNA from as little as 0.5 mg tissue. Purified RNA is suitable for RT-PCR, Northern analysis, differential display, and poly A+ RNA selection.