M-MLV(H-) Reverse Transcriptase Reference: R021-01 Wild-type M-MLV (Moloney Murine Leukemia Virus) has the following activities: RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity; RNase H activity. Because RNase H activity can catalyze the degradation of RNA in the DNA/RNA hybrid strand, the template RNA may be degraded in the cDNA one-strand synthesis reaction. M-MLV (H-) Reverse Transcriptase is an M-MLV mutant with loss of RNase H activity obtained by site-directed mutagenesis. Compared with the common mutants obtained by deleting the RNase H domain, this product retains the complete protein structure, so it has the same polymerase activity as the wild type, and can be used for longer cDNA synthesis and full-length cDNA library Construction, etc. It also has strand displacement activity and can be used in experiments such as 5'RACE.Application: Point mutations eliminate RNase H activity to obtain long cDNA products; Stable and reliable reverse transcription performance for RNA templates above 100 ng; Synthetic fragment ≤5 kb; Can be used for experiments such as 5’-RACE reaction and cDNA library construction
HiScript III Reverse Transcriptase Reference: R302-01 HiScript® III Reverse Transcriptase is an upgraded version of HiScript® II Reverse Transcriptase, which can perform highly efficient reverse transcription reactions at 37°C. HiScript® III. Reverse Transcriptase still retains the thermal stability of the second-generation product HiScript® II Reverse Transcriptase. For RNA with complex secondary structure, the reverse transcription temperature can be increased To 50~55℃, avoid the inhibition of cDNA synthesis by RNA complex secondary structure, and can effectively synthesize high-quality cDNA. In addition, this product still has superior continuous synthesis ability and super Strong impurity tolerance. Application: Extensive template compatibility: compatible with various templates such as animals, plants, viruses, etc.; Super impurity tolerance: It has super tolerance to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid); Excellent reverse transcription efficiency: reverse transcription efficiency is higher than that of second-generation products
HiScript II 1st Strand cDNA Synthesis Kit Reference: R211-01 HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation of HiScript® Reverse Transcriptase, HiScript® II has further greatly improved thermal stability and is very suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript® II adds multiple point mutations, which further enhances template affinity and progress, and has higher tolerance to common reverse transcription inhibitors, which greatly improves the ability to synthesize full-length cDNA. HiScript® II 1st Strand cDNA Synthesis Kit is a first-strand cDNA synthesis kit based on HiScript® II Reverse Transcriptase. It contains all the components needed to synthesize high-quality first-strand cDNA. The product is suitable for subsequent PCR, qPCR and other experiments. 2 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo (dT)23VN included in the kit has a stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo (dT)23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can not only synthesize full-length cDNA (up to 20 kb) for cloning, but also efficiently synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR.Application: Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 1 pg-5 μg total RNA, and can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.
HiScript II 1st Strand cDNA Synthesis Kit Reference: R211-02 HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation of HiScript® Reverse Transcriptase, HiScript® II has further greatly improved thermal stability and is very suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript® II adds multiple point mutations, which further enhances template affinity and progress, and has higher tolerance to common reverse transcription inhibitors, which greatly improves the ability to synthesize full-length cDNA. HiScript® II 1st Strand cDNA Synthesis Kit is a first-strand cDNA synthesis kit based on HiScript® II Reverse Transcriptase. It contains all the components needed to synthesize high-quality first-strand cDNA. The product is suitable for subsequent PCR, qPCR and other experiments. 2 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo (dT)23VN included in the kit has a stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo (dT)23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can not only synthesize full-length cDNA (up to 20 kb) for cloning, but also efficiently synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR.Application: Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 1 pg-5 μg total RNA, and can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.
Pyrophosphatase, Inorganic (E. coli) Reference: K1087-10 Pyrophosphatase, inorganic (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphates