Category: Molecular Biology Reagents

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  • Categories: DNA polymerases
  • Categories: Sample collection
Reference: ISWAB‐ID‐250

Non-invasive sample collection tube for collection and stabilization of protein and DNA in the same oral sample. iSWAB-ID is an efficient sample collection system which enables long term room temperature stabilization of the collected sample at the point of collection, while ensuring proper chain of custody. This system allows for maximizing sample recovery and obtaining human DNA compatible with ID profiling assays. DNA extraction can be performed using any commercially available whole blood extraction chemistry.

Reference: PCE0201

highQu Taq DNA Polymerase is the classical enzyme for routine PCR applications providing high amplification yields of 3-5 kb targets under various conditions. Taq DNA Polymerase is purified from a recombinant E. coli strain carrying the Taq DNA polymerase gene. Taq DNA polymerase is thermostable 5´ → 3´ DNA polymerase. It lacks 3´ → 5´ exonuclease (proofreading) activity and has low 5´ → 3´ exonuclease activity. Polymerase exhibits deoxynucleotidyl transferase activity resulting in A-overhang at the 3'-ends of PCR products and allowing for TA cloning. The PCR accuracy of Taq DNA Polymerase is 4.5 x 104 (a number of incorporated nucleotides before the first error occurs). The supplied reaction buffer for most flexibility in optimization, contains no dNTPs and no magnesium. The 50 mM MgCl2 solution is provided separately what allows for magnesium optimization starting from 1 mM up to 4 mM final concentration upon the need. The dNTPs in sets or mixes can be purchased from highQu separately.

Reference: ISWAB‐BL‐250

iSWAB-Blood is an efficient system for the concentration and long term room temperature stabilization of gDNA and analytes from blood drops. Investigation of  metabolic genetic disorders has traditionally been performed by collecting several blood drops through a minimally invasive procedure called a heel prick. This technique involves spotting 5-6 blood drops on filter paper.

Reference: PCE0202

highQu Taq DNA Polymerase is the classical enzyme for routine PCR applications providing high amplification yields of 3-5 kb targets under various conditions. Taq DNA Polymerase is purified from a recombinant E. coli strain carrying the Taq DNA polymerase gene. Taq DNA polymerase is thermostable 5´ → 3´ DNA polymerase. It lacks 3´ → 5´ exonuclease (proofreading) activity and has low 5´ → 3´ exonuclease activity. Polymerase exhibits deoxynucleotidyl transferase activity resulting in A-overhang at the 3'-ends of PCR products and allowing for TA cloning. The PCR accuracy of Taq DNA Polymerase is 4.5 x 104 (a number of incorporated nucleotides before the first error occurs). The supplied reaction buffer for most flexibility in optimization, contains no dNTPs and no magnesium. The 50 mM MgCl2 solution is provided separately what allows for magnesium optimization starting from 1 mM up to 4 mM final concentration upon the need. The dNTPs in sets or mixes can be purchased from highQu separately.

Reference: iSWAB‐MB‐1200

iSWAB-Microbiome has been proven to efficiently inactivate bacteria, fungi, spores, and DNA/RNA viruses allowing safer transport of biological samples to processing labs, especially when samples are subject to long transit times. DNA and RNA from collected microorganisms remains stable at room temperature for up to 40 days for DNA, and 21 days for RNA, eliminating the need for expedited cold chain transport.

Reference: HSE0101

highQu ALLin™ Hot Start Taq Polymerase is the superior sensitive hot-start DNA polymerase. The enzyme activity at room temperature is blocked by a small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation occurs and no background appears. In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of complex or longer templates and fast cycling. ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the ALLin™ Hot Start Taq Mastermix, 2X is available.

Reference: iSWAB‐MBF‐1200

iSWAB-Microbiome has been proven to efficiently inactivate bacteria, fungi, spores, and DNA/RNA viruses allowing safer transport of biological samples to processing labs, especially when samples are subject to long transit times. DNA and RNA from collected microorganisms remains stable at room temperature for up to 40 days for DNA, and 21 days for RNA, eliminating the need for expedited cold chain transport.

Reference: HSE0105

highQu ALLin™ Hot Start Taq Polymerase is the superior sensitive hot-start DNA polymerase. The enzyme activity at room temperature is blocked by a small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation occurs and no background appears. In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of complex or longer templates and fast cycling. ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the ALLin™ Hot Start Taq Mastermix, 2X is available.

Reference: ISWAB‐CELLS‐1200

Multiomics Enabled (including RNA) From the Same Sample: - iSWAB Cells is designed for non-invasive, self-collection of intact and viable buccal cells. -Cell Stabilization System is optimized for room temperature stabilization of viable cells up to 4 weeks. -Room temperature transportation allows for wider bio-sample accessibility, and enables scientific research collaborations at a global level which greatly enhances our understanding of wellness/health and disease

Reference: HSM0201

highQu ALLin™ Hot Start Taq DNA Polymerase is the superior sensitive enzyme The activity at room temperature is blocked by small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation and no background. In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of complex or longer templates and fast cycling. ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Hot Start Taq DNA Polymerase is maximized by the use of 2X Mastermix providing the additional advantage of reduced pipetting and minimized errors. The mastermix is even supplied with PCR Water, and the only thing to add is the template with primers.

Reference: ISD‐T‐1200‐R

DNA collection kit for humans that can capture samples producing high yields of double stranded, long fragmented DNA from buccal cells using traditional swabs.

Reference: HSM0205

highQu ALLin™ Hot Start Taq DNA Polymerase is the superior sensitive enzyme The activity at room temperature is blocked by small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation and no background. In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of complex or longer templates and fast cycling. ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Hot Start Taq DNA Polymerase is maximized by the use of 2X Mastermix providing the additional advantage of reduced pipetting and minimized errors. The mastermix is even supplied with PCR Water, and the only thing to add is the template with primers.