Heat-labile UDG Reference: P051-02 UDG (Uracil-DNA Glycosylase, uracil-DNA glycosylase) can catalyze the hydrolysis of dU-containing DNA single-stranded or double-stranded uracil base and sugar phosphate backbone N-glycosidic bond, release free uracil, thereby The resulting abasic sites are easily broken by hydrolysis. Heat-labile UDG is derived from psychrophilic marine bacteria and is sensitive to high temperature. It can irreversibly inactivate enzymes above 50℃. It is suitable for PCR/QPCR and RT-PCR/RT-QPCR systems.Application: 55°C 10 min rapid inactivation; Compatible with common PCR reaction systems
MiPure Cell/Tissue miRNA Kit Reference: RC201 MiPure Cell / Tissue miRNA Kit is suitable for extracting small RNA (miRNA, <200 nt) from less than 5 x 106 eukaryotic cells or less than 100 mg animal tissues. The resulting miRNA has high purity without large RNA or genome DNA contamination can be directly used for chip analysis, Northern hybridization, RT-PCR and miRNA library construction. This kit combines high-efficiency RNA Isolater extraction reagents and silica gel column purification technology to complete the extraction of miRNA within 1 hour; it can also be used to separate and extract large molecular RNA (>200 nt) or total RNA (including miRNA) to satisfy users Different needs.Application: The miRNA extraction efficiency is high, and the starting amount is wide; Larger RNA and genomic DNA have fewer residues; Easy to operate, strong compatibility
E.coli UDG Reference: P061-01 E. coli uracil-DNA glycosylase (UDG) catalyzes the release of uracil from uracil-containing DNA. UDG can effectively hydrolyze uracil on single-stranded or double-stranded DNA, but cannot hydrolyze uracil from oligonucleotides of 6 bases or less.Application: After strict quality control
FastPure Plant Total RNA Isolation Kit (Polysaccharides / Polyphenolics-Rich) Reference: RC401 This kit can quickly extract the total RNA of multiple samples from plant tissues, especially plant tissues rich in polysaccharides and polyphenols (such as cotton leaves, pine needles, bananas, potato tubers, grapes, rose flowers, buckwheat, etc.). The kit is based on silica gel column purification technology. There is no need to use toxic phenol-chloroform extraction during the extraction process. The whole process only takes 30 minutes. The FastPure gDNA-Filter Column II in the kit can effectively filter impurities and specifically adsorb and remove genomic DNA. FastPure RNA Column Ⅳ can efficiently bind RNA, and is equipped with optimized Buffer solution, so that the total RNA obtained is of high purity, free of protein and other impurities, and can be used for RT-PCR, Real-time PCR, chip analysis, Northern Blot And other downstream experiments.
E.coli UDG Reference: P061-02 E. coli uracil-DNA glycosylase (UDG) catalyzes the release of uracil from uracil-containing DNA. UDG can effectively hydrolyze uracil on single-stranded or double-stranded DNA, but cannot hydrolyze uracil from oligonucleotides of 6 bases or less.Application: After strict quality control
Murine RNase Inhibitor Reference: R301-01 Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript® II Reverse Transcriptase, HiScript® Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).Application: The enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions; High-purity E. coli soluble expression, no RNase residue, compatible with RT-PCR/qPCR system
Murine RNase Inhibitor Reference: R301-02 Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript® II Reverse Transcriptase, HiScript® Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).Application: The enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions; High-purity E. coli soluble expression, no RNase residue, compatible with RT-PCR/qPCR system
Murine RNase Inhibitor Reference: R301-03 Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript® II Reverse Transcriptase, HiScript® Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).Application: The enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions; High-purity E. coli soluble expression, no RNase residue, compatible with RT-PCR/qPCR system
VeZol Reagent Reference: R411-01 VeZol Reagent is intended for the isolation of high-quality total RNA from cultured cells, animal tissues, and plant tissues. VeZol Reagent is a monophasic solution of phenol and guanidine isothiocyanate supplemented with optimized proprietary components for more effective sample disruption and RNase inhibition. After rapid sample lysis with VeZol Reagent and chloroform extraction, the lysate separates into an upper aqueous phase (containing RNA), an interphase, and a lower red organic phase, and total RNA can be obtained in high yield and purity by isopropanol precipitation and ethanol wash. The resulting total RNA can be used directly for RT-PCR, RT-qPCR, Northern blot, Dot blot, in vitro translation, high-throughput sequencing, and other molecular biology experiments.FeaturesUltra classical organic extractionSuper lysis capabilityRNA extraction from a wide variety of samples are available, such as cells, tissues, simple plants and blood.