E.Z.N.A.® Stool DNA Kit Reference: D4015-02 The E.Z.N.A.® Stool DNA Kit allows rapid and reliable isolation of high-quality total DNA from fresh and frozen stool samples. Up to 200 mg of stool samples can be processed in less than 60 minutes. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of spin column technology to eliminate humic acid, polysaccharides, phenolic compounds, and enzyme inhibitors from stool samples. Purified DNA is suitable for PCR, restriction digestion, and hybridization technique.
SQ Blood DNA Kit Reference: D5032-00 The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen, and anti-coagulated whole blood. The method can also be used for the preparation of genomic DNA from buffy coat, bone marrow or cultured cells. The kit allows single or multiple, simultaneous processing of samples in under 90 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.® SQ Blood DNA method is ready for applications such as PCR, Southern blotting, and restriction digestion.
VeZol Reagent Reference: R411-01 VeZol Reagent is intended for the isolation of high-quality total RNA from cultured cells, animal tissues, and plant tissues. VeZol Reagent is a monophasic solution of phenol and guanidine isothiocyanate supplemented with optimized proprietary components for more effective sample disruption and RNase inhibition. After rapid sample lysis with VeZol Reagent and chloroform extraction, the lysate separates into an upper aqueous phase (containing RNA), an interphase, and a lower red organic phase, and total RNA can be obtained in high yield and purity by isopropanol precipitation and ethanol wash. The resulting total RNA can be used directly for RT-PCR, RT-qPCR, Northern blot, Dot blot, in vitro translation, high-throughput sequencing, and other molecular biology experiments.FeaturesUltra classical organic extractionSuper lysis capabilityRNA extraction from a wide variety of samples are available, such as cells, tissues, simple plants and blood.
SQ Blood DNA Kit Reference: D5032-03 The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen, and anti-coagulated whole blood. The method can also be used for the preparation of genomic DNA from buffy coat, bone marrow or cultured cells. The kit allows single or multiple, simultaneous processing of samples in under 90 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.® SQ Blood DNA method is ready for applications such as PCR, Southern blotting, and restriction digestion.
VeZol Reagent Reference: R411-02 VeZol Reagent is intended for the isolation of high-quality total RNA from cultured cells, animal tissues, and plant tissues. VeZol Reagent is a monophasic solution of phenol and guanidine isothiocyanate supplemented with optimized proprietary components for more effective sample disruption and RNase inhibition. After rapid sample lysis with VeZol Reagent and chloroform extraction, the lysate separates into an upper aqueous phase (containing RNA), an interphase, and a lower red organic phase, and total RNA can be obtained in high yield and purity by isopropanol precipitation and ethanol wash. The resulting total RNA can be used directly for RT-PCR, RT-qPCR, Northern blot, Dot blot, in vitro translation, high-throughput sequencing, and other molecular biology experiments.FeaturesUltra classical organic extractionSuper lysis capabilityRNA extraction from a wide variety of samples are available, such as cells, tissues, simple plants and blood.
E.Z.N.A.® SP Plant DNA Kit Reference: D5511-00 The E.Z.N.A.® SP Plant DNA Kit is specially designed for the rapid and reliable isolation of high-quality total cellular DNA from a variety of plant species and tissues. Up to 100 mg of fresh sample or 30 mg of dry sample can be processed in less than 40 minutes. The optimized procedure incorporates the homogenizer spin column, a unique filter filtration and homogenization column that can efficiently remove cell debris and improve the sample handling following the sample lysis. Purified DNA is suitable for most downstream applications to include PCR, restriction enzyme digestion, and hybridization techniques.
FreeZol Reagent Reference: R711-01 FreeZol Reagent is widely applicable to the extraction of total RNA from cultured cells, animal tissues, and simple plant tissues. Compared with the conventional Trizol method, this product features a simple procedure that can be performed at room temperature with no need to use chloroform for phase separation. In addition, this product ensures the integrity and purity of the extracted RNA by precipitating proteins, DNA, polysaccharides and other impurities in the organic phase while retaining RNA in the upper aqueous phase. The whole procedure can be completed in 1 h. The obtained total RNA can be used directly for RT-PCR, qRT-PCR, Northern blot, Dot blot, in vitro translation, NGS, and other molecular biology experiments.Features Safe and low in toxicity: No need for toxic and harmful reagents such as chloroform and β-mercaptoethanolEase to use: RNA extraction in a monophasic system, no phase separation, all operations at room temperatureHigh RNA quality: High purity and integrity of RNA at a yield comparable to the traditional Trizol method
E.Z.N.A.® SP Plant DNA Kit Reference: D5511-01 The E.Z.N.A.® SP Plant DNA Kit is specially designed for the rapid and reliable isolation of high-quality total cellular DNA from a variety of plant species and tissues. Up to 100 mg of fresh sample or 30 mg of dry sample can be processed in less than 40 minutes. The optimized procedure incorporates the homogenizer spin column, a unique filter filtration and homogenization column that can efficiently remove cell debris and improve the sample handling following the sample lysis. Purified DNA is suitable for most downstream applications to include PCR, restriction enzyme digestion, and hybridization techniques.
FastPure Universal Plant Total RNA Isolation Kit Reference: RC411-01 FastPure Universal Plant Total RNA Isolation Kit can be used to quickly extract total RNA from plant tissues. The kit includes two solution systems designed for simple RNA extraction from a variety of plant tissues (wheat, rice, corn, Arabidopsis thaliana, tobacco, rape, etc.), polysaccharide and polyphenol rich plant tissues (cotton leaf, soybean leaf, pine needle, ginkgo leaf, fig leaf, gardenia leaf, wheat seed, corn seed, red bean seed, potato, sweet potato, soybean seed, sesame seed, peanut seeds, rapeseed, etc.), fruit pulp (watermelon, apple, peach, pear, banana, mango, etc.), and fungi (shiitake, button mushroom, oyster mushroom, Neurospora crassa, etc.). The kit is based on silica column purification technology and does not involve toxic reagents (e.g., phenol or chloroform and β-mercaptoethanol) or time-consuming alcohol precipitation. The whole extraction process takes only 11 min. FastPure gDNA-Filter Columns III in the kit effectively remove impurities and gDNA. FastPure RNA Columns V efficiently bind to RNA and, paired with optimized buffers, yield high-purity total RNA with minimal gDNA residue and no protein or other impurities that can be used for a range of downstream experiments, including RT-PCR, real-time PCR, RNA library preparation, microarray analysis, Northern blot, dot blot, and molecular cloning.
E.Z.N.A.® SP Plant DNA Kit Reference: D5511-02 The E.Z.N.A.® SP Plant DNA Kit is specially designed for the rapid and reliable isolation of high-quality total cellular DNA from a variety of plant species and tissues. Up to 100 mg of fresh sample or 30 mg of dry sample can be processed in less than 40 minutes. The optimized procedure incorporates the homogenizer spin column, a unique filter filtration and homogenization column that can efficiently remove cell debris and improve the sample handling following the sample lysis. Purified DNA is suitable for most downstream applications to include PCR, restriction enzyme digestion, and hybridization techniques.