Category: Molecular Biology Reagents

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  • Categories: DNA Extraction
  • Categories: Food and Water assays
Reference: DC301-01

This kit uses an optimized buffer system and silica gel column purification technology, which can recover 70 bp-20 kb DNA fragments from various concentrations of TAE or TBE agarose gels, and transfer them to the DNA adsorption column under high salt conditions. Direct centrifugation can specifically adsorb DNA and remove other impurities. In addition, this kit can directly purify DNA fragments from PCR products, enzymatic reaction systems or crude DNA products obtained by various other methods, while removing impurities such as proteins, other organic compounds, inorganic salt ions, and oligonucleotide primers. . It can ensure that the purification work is completed in 10-15 min. The purified DNA can be directly used for molecular biology research such as ligation, transformation, restriction enzyme digestion, in vitro transcription, PCR, sequencing, and microinjection.Application: Simple and fast; One box for dual use; High recovery efficiency; Wide range of recovered DNA fragments

Reference: DC105-01

FastPure FFPE DNA Isolation Kit is intended for isolating genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue, overcoming the inhibitory effect caused by formalin crosslinking. The deparaffinization solution in this kit is safer than xylene. The DNA-specific spin column and the unique buffer system also ensure the high quality of obtained DNA. The whole extraction process takes only 20 min (digestion time not counted). The extracted genomic DNA is of good integrity, high purity, and stable quality and can be used for a wide range of downstream applications, including PCR, qPCR, and library preparation.

Reference: DC201-01

FastPure Plasmid Mini Kit purifies 1 - 5 ml of cultured bacteria and lyses the cells by an optimized SDS-alkaline method. The kit utilizes a silica-based membrane technology in the form of a convenient spin column, and binds plasmid DNA in a highly efficient manner under high-salt and low-pH conditions and largely remove proteins, genomic DNA, RNA, and other impurities. In the end, pure plasmid DNA is eluted from the silica membrane with a lowsalt, high-pH elution buffer. The kit recovers up to 35 µg of high-copy plasmid DNA per isolation procedure.The purified plasmid DNA is ready for use in biological experiments such as enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of common passage cells.

Reference: DC203-01

This kit is intended for extraction from 1 - 5 ml of overnight bacterial cultures. It adopts anoptimized alkaline lysis method and features a unique silica membrane adsorption technology and special Buffer ERB and Buffer ERW for specific binding of plasmids and effective removal of endotoxins, proteins, and other impurities. The whole extraction process takes only 25 min. The extracted plasmid DNA contains minimal endotoxin residue (≤0.1 EU/μg) and can be used for the transfection of various types of cells and common experiments such as enzyme digestion, PCR, sequencing, and ligation.

Reference: DC502-01

This kit is intended for fast isolation and purification of host and microbiome DNA from blood, other biological fluids (alveolar lavage fluid, sputum, cerebrospinal fluid, etc.), swab, bacteria/fungi culture, and animal tissue. The kit combines chemical and mechanical lysis methods and can efficiently lyse bacterial and fungal cells with thick cell walls. The extraction process with this kit is safe, fast, and simple as neither toxic reagents (e.g., phenol and chloroform) nor time-consuming procedures (such as alcohol precipitation and RNase treatment) are required. While ensuring efficiency in nucleic acid extraction, the kit also minimizes the contamination of RNA, proteins, lipids, and other impurities. The isolated DNA is suitable for various downstream applications, including PCR, real-time PCR, metagenomic library preparation, and microarray analysis.

Reference: RC323-01

FastPure Viral DNA/RNA Mini Kit Pro is a column extraction kit, which can extract viral DNA/RNA from multiple types of samples. The kit uses a unique lysis system, without high temperature incubation, and can significantly improve the recovery rate of trace nucleic acid with carrier RNA. After specific adsorption on silica gel membrane, viral DNA/RNA can be quickly and efficiently purified. For a single sample, it only takes 12 minutes to finish extraction. And the products can be directly used for downstream related experiments such as reverse transcription, PCR, Real-Time PCR, NGS and Northern blot.

Reference: DC205-01

This kit is applicable for extracting bacterial culture that has been cultured overnight in a volume  of 15 - 50 ml. It uses an optimized alkaline lysis method to lyse cells and utilizes a unique silica  membrane adsorption technique along with special Buffer ERB and Buffer ERW solutions to  specifically bind plasmids and effectively remove impurities such as endotoxins, proteins, and  genomic DNA. Additionally, the unique P2 solution with indicator can indicate the completeness of lysis and neutralization through color changes, ensuring extraction quality and enabling visual  operation. The extracted plasmid DNA has extremely low residual endotoxin levels, making it suitable for various cell transfection and routine operations, including restriction endonuclease digestion, PCR, sequencing, ligation, and other experiments.

Reference: MT-ISO

The RayBio® Mitochondrial DNA Isolation Kit is a simple and effective tool for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from nuclear genomic DNA.