ClonExpress Ultra One Step Cloning Kit V3 Reference: C117-02 ClonExpress Ultra One Step Cloning Kit V3 utilizes the latest optimized technology, enabling highly efficient homologous recombination of 1 - 11 DNA fragments. The newly optimized 2 × CE Mix V3 not only ensures a high cloning success rate for complicated situations such as large vectors and GC-rich/poor homologous arms, but also significantly enhances compatibility with amounts of DNA input. This greatly solves the problem of reduced cloning efficiency caused by low DNA fragment concentration.FeaturesRobust performance: 1 - 11 fragments assembly; the positive rate is > 95%High Efficiency: 5 - 30 min rapid recombination; high clones and positivity rateSpecial optimization: Maximum compatible with 15 - 35 bp overlaps, not only 15 - 20 bpBroad Compatibility: Suitable for ultra-low input, GC-rich/poor overlaps, and low-purity samples
dTTP (100 mM) Reference: P035-01 These products are high-purity single-component dUTP/dATP/dTTP/dCTP/dGTP, each at a concentration of 100 mM, which can be used for PCR, real-time PCR, RT-PCR, primer extension reaction, DNA sequencing, DNA labeling and other conventional reactions used in molecular biology.Application:
E.Z.N.A.® Tissue DNA Kit Reference: D3396-01 The E.Z.N.A.® Tissue DNA Kit offers a versatile and cost-effective method for the isolation of DNA from a wide variety of samples including fresh or frozen animal cultured cells and tissues, buccal swabs, whole blood, mouse tail snips, etc. The DNA purification process is simplified with HiBind® Mini Column technology into four quick lyse-bind-wash-elute†steps and can be accomplished in less than 20 minutes post-lysis. This convenient spin-column format avoids time-consuming steps like alcohol precipitation, use of toxic compounds such as phenol and chloroform and allows for multiple samples to be processed in parallel. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion.
Seamless Gene Cloning Kit Reference: 230-K6004 The RayBio® Seamless Gene Cloning Kit is a one enzyme-introduced constitutive gene cloning tool designed to deliver no gap connection among DNA fragments and vectors using a homologous recombination technique.
dCTP (100 mM) Reference: P036-01 These products are high-purity single-component dUTP/dATP/dTTP/dCTP/dGTP, each at a concentration of 100 mM, which can be used for PCR, real-time PCR, RT-PCR, primer extension reaction, DNA sequencing, DNA labeling and other conventional reactions used in molecular biology.Application:
E.Z.N.A.® FFPE DNA Kit Reference: D3399-00 The E.Z.N.A.® FFPE DNA Kit is designed for fast and easy purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Paraffin removal can be performed using a xylene and ethanol method or efficient heat treatment. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking, effectively releasing short and long DNA fragments. After adjusting the binding conditions with ethanol, the lysate is applied to the MicroElute DNA column to bind DNA. Cellular debris and proteins are effectively removed during the wash steps. High quality purified DNA is then eluted in elution buffer or water and is ready for applications such as PCR and next-generation sequencing.
dGTP (100 mM) Reference: P037-01 These products are high-purity single-component dUTP/dATP/dTTP/dCTP/dGTP, each at a concentration of 100 mM, which can be used for PCR, real-time PCR, RT-PCR, primer extension reaction, DNA sequencing, DNA labeling and other conventional reactions used in molecular biology.Application:
E.Z.N.A.® FFPE DNA Kit Reference: D3399-01 The E.Z.N.A.® FFPE DNA Kit is designed for fast and easy purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Paraffin removal can be performed using a xylene and ethanol method or efficient heat treatment. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking, effectively releasing short and long DNA fragments. After adjusting the binding conditions with ethanol, the lysate is applied to the MicroElute DNA column to bind DNA. Cellular debris and proteins are effectively removed during the wash steps. High quality purified DNA is then eluted in elution buffer or water and is ready for applications such as PCR and next-generation sequencing.
Heat-labile UDG Reference: P051-01 UDG (Uracil-DNA Glycosylase, uracil-DNA glycosylase) can catalyze the hydrolysis of dU-containing DNA single-stranded or double-stranded uracil base and sugar phosphate backbone N-glycosidic bond, release free uracil, thereby The resulting abasic sites are easily broken by hydrolysis. Heat-labile UDG is derived from psychrophilic marine bacteria and is sensitive to high temperature. It can irreversibly inactivate enzymes above 50℃. It is suitable for PCR/QPCR and RT-PCR/RT-QPCR systems.Application: 55°C 10 min rapid inactivation; Compatible with common PCR reaction systems
E.Z.N.A.® Plant DNA Kit Reference: D3485-00 The E.Z.N.A.® Plant DNA Kit is designed for the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of plant species. Up to 100 mg of wet samples or 30 mg dry samples can be processed in less than 60 minutes. This kit uses a novel and proprietary method to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant sample. Purified DNA is suitable for PCR, restriction enzyme digestion, and hybridization techniques. There are no organic extractions, thus reducing plastic waste and hands-on time.
Heat-labile UDG Reference: P051-02 UDG (Uracil-DNA Glycosylase, uracil-DNA glycosylase) can catalyze the hydrolysis of dU-containing DNA single-stranded or double-stranded uracil base and sugar phosphate backbone N-glycosidic bond, release free uracil, thereby The resulting abasic sites are easily broken by hydrolysis. Heat-labile UDG is derived from psychrophilic marine bacteria and is sensitive to high temperature. It can irreversibly inactivate enzymes above 50℃. It is suitable for PCR/QPCR and RT-PCR/RT-QPCR systems.Application: 55°C 10 min rapid inactivation; Compatible with common PCR reaction systems