E.Z.N.A.® Stool DNA Kit Reference: D4015-02 The E.Z.N.A.® Stool DNA Kit allows rapid and reliable isolation of high-quality total DNA from fresh and frozen stool samples. Up to 200 mg of stool samples can be processed in less than 60 minutes. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of spin column technology to eliminate humic acid, polysaccharides, phenolic compounds, and enzyme inhibitors from stool samples. Purified DNA is suitable for PCR, restriction digestion, and hybridization technique.
Murine RNase Inhibitor Reference: R301-03 Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript® II Reverse Transcriptase, HiScript® Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).Application: The enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions; High-purity E. coli soluble expression, no RNase residue, compatible with RT-PCR/qPCR system
SQ Blood DNA Kit Reference: D5032-00 The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen, and anti-coagulated whole blood. The method can also be used for the preparation of genomic DNA from buffy coat, bone marrow or cultured cells. The kit allows single or multiple, simultaneous processing of samples in under 90 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.® SQ Blood DNA method is ready for applications such as PCR, Southern blotting, and restriction digestion.
RoomTemp Sample Lysis Kit Reference: P073-01 RoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer, among which Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can be fully released The genomic DNA in the cell, Stabilizing Buffer is added with protective proteins and stabilizing factors, which can eliminate the inhibitory effect of the inhibitor in the lysed sample on the downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include Fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, sodium heparin, etc.). Genomic DNA can be released from whole blood samples after 3 minutes of lysis at room temperature. The lysed DNA solution can be directly used as a template ，Apply to Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification, etc. It can achieve the same effect as traditional genome extraction methods without complicated extraction operations. In addition to blood, this kit can also Compatible with pyrolysis FTA blood cards, oral swabs, plant tissues and other samples. Application: Simple operation: 3 minutes at room temperature, no need for complex template extraction. High lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome. Wide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.
SQ Blood DNA Kit Reference: D5032-03 The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen, and anti-coagulated whole blood. The method can also be used for the preparation of genomic DNA from buffy coat, bone marrow or cultured cells. The kit allows single or multiple, simultaneous processing of samples in under 90 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.® SQ Blood DNA method is ready for applications such as PCR, Southern blotting, and restriction digestion.
RoomTemp Sample Lysis Kit Reference: P073-02 RoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer. Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can fully release the genomic DNA in the cell. Stabilizing Buffer adds protective proteins and stabilizing factors. Eliminate the inhibitory effect of inhibitors in the lysed sample on downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, heparin sodium, etc.). After lysis at room temperature for 3 minutes, genomic DNA can be released from the whole blood sample. The lysed DNA solution can be directly used as a template for SNP detection by Taqman ® probe method, qPCR probe method quantification, PCR amplification, etc. Without complicated extraction operations, it can achieve the same effect as traditional genome extraction methods. In addition to blood, this kit is also compatible with pyrolysis FTA blood cards, buccal swabs, plant tissues and other samples.Application: Simple operation: 3 minutes at room temperature, no need for complex template extraction. High lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome. Wide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.
E.Z.N.A.® SP Plant DNA Kit Reference: D5511-00 The E.Z.N.A.® SP Plant DNA Kit is specially designed for the rapid and reliable isolation of high-quality total cellular DNA from a variety of plant species and tissues. Up to 100 mg of fresh sample or 30 mg of dry sample can be processed in less than 40 minutes. The optimized procedure incorporates the homogenizer spin column, a unique filter filtration and homogenization column that can efficiently remove cell debris and improve the sample handling following the sample lysis. Purified DNA is suitable for most downstream applications to include PCR, restriction enzyme digestion, and hybridization techniques.
RoomTemp Sample Lysis Kit Reference: P073-03 RoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer. Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can fully release the genomic DNA in the cell. Stabilizing Buffer adds protective proteins and stabilizing factors. Eliminate the inhibitory effect of inhibitors in the lysed sample on downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, heparin sodium, etc.). After lysis at room temperature for 3 minutes, genomic DNA can be released from the whole blood sample. The lysed DNA solution can be directly used as a template for SNP detection by Taqman ® probe method, qPCR probe method quantification, PCR amplification, etc. Without complicated extraction operations, it can achieve the same effect as traditional genome extraction methods. In addition to blood, this kit is also compatible with pyrolysis FTA blood cards, buccal swabs, plant tissues and other samples.Application: Simple operation: 3 minutes at room temperature, no need for complex template extraction. High lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome. Wide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.
E.Z.N.A.® SP Plant DNA Kit Reference: D5511-01 The E.Z.N.A.® SP Plant DNA Kit is specially designed for the rapid and reliable isolation of high-quality total cellular DNA from a variety of plant species and tissues. Up to 100 mg of fresh sample or 30 mg of dry sample can be processed in less than 40 minutes. The optimized procedure incorporates the homogenizer spin column, a unique filter filtration and homogenization column that can efficiently remove cell debris and improve the sample handling following the sample lysis. Purified DNA is suitable for most downstream applications to include PCR, restriction enzyme digestion, and hybridization techniques.
RNA Keeper Tissue Stabilizer Reference: R501-01 RNA Keeper Tissue Stabilizer is a non-toxic solution that can quickly penetrate into tissues, inactivate endogenous RNase, immediately stabilize and protect the integrity of RNA. Fresh tissue samples do not need liquid nitrogen quick freezing, as long as they are immersed in RNA Keeper Tissue Stabilizer, they can be stored at 37°C for 1 day, room temperature for 1 week, 4°C for 4 weeks, or long-term storage at -20°C/-80°C, and repeated Freezing and thawing will not significantly affect the integrity of RNA. The samples stored in the RNA Keeper Tissue Stabilizer can be directly used to extract RNA using RNA isolater total RNA extraction reagent (R401-01) or spin column method. RNA Keeper Tissue Stabilizer can be used for the preservation of brain, heart, liver, pancreas, kidney, spleen, testis, muscle and other tissues.Application: Ultra-high amplification specificity: AceTaq® DNA Polymerase based on chemical hot-start, completely blocked enzyme activity before 95°C, and equipped with a patented specific promotion factor Exactor, which makes amplification more specific;
E.Z.N.A.® SP Plant DNA Kit Reference: D5511-02 The E.Z.N.A.® SP Plant DNA Kit is specially designed for the rapid and reliable isolation of high-quality total cellular DNA from a variety of plant species and tissues. Up to 100 mg of fresh sample or 30 mg of dry sample can be processed in less than 40 minutes. The optimized procedure incorporates the homogenizer spin column, a unique filter filtration and homogenization column that can efficiently remove cell debris and improve the sample handling following the sample lysis. Purified DNA is suitable for most downstream applications to include PCR, restriction enzyme digestion, and hybridization techniques.
DNase I , RNase-free Reference: EN401-01 DNase I (deoxyribonuclease I), that is, Deoxyribonuclease I, is an endodeoxyribonuclease that can digest single-stranded or double-stranded DNA. It recognizes and cleaves phosphodiester bonds to produce a 5'-end phosphate group Group, 3'-end hydroxyl monodeoxynucleotide or single-stranded or double-stranded oligodeoxynucleotide. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions Mg2+, Mn2+, etc. It exists in Mg2+ In the case of Mn2+, the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA; and in the presence of Mn2+, it can recognize and cut almost the same site on both strands of DNA, resulting in blunt ends or There are sticky-end DNA fragments with 1-2 nucleotides protruding. This product has undergone strict quality control and is suitable for RNA extraction, in vitro transcription, DNA removal in RT-PCR experiments, DNase I footprinting, nick translatioin, DNA random fragment library construction and other molecular biology experiment. Application: High activity Very low RNase residue Good stability