E.Z.N.A.® FFPE RNA Kit Reference: R6954-00 The E.Z.N.A.® FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscle tissue. The sample is first processed in a special buffer by heating to deactivate RNases and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then applied to a HiBind RNA column to capture RNA. After two quick wash steps, purified RNA is eluted with water. While E.Z.N.A.® FFPE RNA Kit is optimized to minimize the effect of formaldehyde modification, it is not recommended to use the RNA purified from this kit for downstream applications that require full-length RNA.
Ultra-Universal TOPO Cloning Kit Reference: C603-01 Ultra-Universal TOPO Cloning Kit is the fourth-generation TOPO cloning kit. The kit uses upgraded Topoisomerase with the optimal buffer and has higher enzyme activity. The addition of the second-generation blunting factor and vector self-ligation inhibitor makes this product compatible with both TA cloning and blunt-end cloning, and increases the positive rate of cloning.Features - Upgraded version: 4th Generation topoisomerase; higher positive rate and colony counts- Fast: Cloning of either sticky-end or blunt-end DNA in only 5 minutes- Efficient: High efficiency cloning and positive rate>95%- Vector self-ligation inhibitor factor: Effectively prevent vector self-ligation and reduce false positives
E.Z.N.A.® FFPE RNA Kit Reference: R6954-01 The E.Z.N.A.® FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscle tissue. The sample is first processed in a special buffer by heating to deactivate RNases and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then applied to a HiBind RNA column to capture RNA. After two quick wash steps, purified RNA is eluted with water. While E.Z.N.A.® FFPE RNA Kit is optimized to minimize the effect of formaldehyde modification, it is not recommended to use the RNA purified from this kit for downstream applications that require full-length RNA.
Ultra-Universal TOPO Cloning Kit Reference: C603-02 Ultra-Universal TOPO Cloning Kit is the fourth-generation TOPO cloning kit. The kit uses upgraded Topoisomerase with the optimal buffer and has higher enzyme activity. The addition of the second-generation blunting factor and vector self-ligation inhibitor makes this product compatible with both TA cloning and blunt-end cloning, and increases the positive rate of cloning.Features - Upgraded version: 4th Generation topoisomerase; higher positive rate and colony counts- Fast: Cloning of either sticky-end or blunt-end DNA in only 5 minutes- Efficient: High efficiency cloning and positive rate>95%- Vector self-ligation inhibitor factor: Effectively prevent vector self-ligation and reduce false positives
E.Z.N.A.® Micro RNA Kit Reference: R7034-01 The E.Z.N.A.® Micro RNA Kit combines the reversible binding properties of the HiBind® matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications. The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is applied to a HiBind® RNA column where large RNA binds. The flow-through is retained and applied to a HiBind® Micro RNA column to bind small RNA.
ClonExpress Ultra One Step Cloning Kit V3 Reference: C117-01 ClonExpress Ultra One Step Cloning Kit V3 utilizes the latest optimized technology, enabling highly efficient homologous recombination of 1 - 11 DNA fragments. The newly optimized 2 × CE Mix V3 not only ensures a high cloning success rate for complicated situations such as large vectors and GC-rich/poor homologous arms, but also significantly enhances compatibility with amounts of DNA input. This greatly solves the problem of reduced cloning efficiency caused by low DNA fragment concentration.FeaturesRobust performance: 1 - 11 fragments assembly; the positive rate is > 95%High Efficiency: 5 - 30 min rapid recombination; high clones and positivity rateSpecial optimization: Maximum compatible with 15 - 35 bp overlaps, not only 15 - 20 bpBroad Compatibility: Suitable for ultra-low input, GC-rich/poor overlaps, and low-purity samples
HiBind® RNA Mini Columns Reference: RNACOL-01 HiBind® RNA Spin Columns, V-Spin are mini spin columns with a cap that can be used either in microcentrifuges or on vacuum manifolds for purification of RNA.
ClonExpress Ultra One Step Cloning Kit V3 Reference: C117-02 ClonExpress Ultra One Step Cloning Kit V3 utilizes the latest optimized technology, enabling highly efficient homologous recombination of 1 - 11 DNA fragments. The newly optimized 2 × CE Mix V3 not only ensures a high cloning success rate for complicated situations such as large vectors and GC-rich/poor homologous arms, but also significantly enhances compatibility with amounts of DNA input. This greatly solves the problem of reduced cloning efficiency caused by low DNA fragment concentration.FeaturesRobust performance: 1 - 11 fragments assembly; the positive rate is > 95%High Efficiency: 5 - 30 min rapid recombination; high clones and positivity rateSpecial optimization: Maximum compatible with 15 - 35 bp overlaps, not only 15 - 20 bpBroad Compatibility: Suitable for ultra-low input, GC-rich/poor overlaps, and low-purity samples
HiBind® RNA Mini Columns Reference: RNACOL-02 HiBind® RNA Spin Columns, V-Spin are mini spin columns with a cap that can be used either in microcentrifuges or on vacuum manifolds for purification of RNA.
Seamless Gene Cloning Kit Reference: 230-K6004 The RayBio® Seamless Gene Cloning Kit is a one enzyme-introduced constitutive gene cloning tool designed to deliver no gap connection among DNA fragments and vectors using a homologous recombination technique.
RNA isolater Total RNA Extraction Reagent Reference: R401-01 RNA isolater Total RNA Extraction Reagent, based on guanidine isothiocyanate and phenol, has a strong lysis ability, which can lyse cells and tissue samples in a short time to ensure the integrity of RNA. This product is widely used for culturing cells, animal tissues, microorganisms, and plant tissues with less secondary metabolism, such as seedlings and young leaves. The sample can be fully lysed in RNA isolater. After centrifugation with chloroform, the solution will form a supernatant layer, an intermediate layer and an organic layer (bright red lower layer). RNA is distributed in the upper aqueous phase. After the supernatant layer is collected, the Total RNA can be recovered by propanol precipitation.Application: Based on guanidine isothiocyanate and phenol, strong cracking ability; The optimized lysate formula has a wide range of sample adaptability.