dGTP (100 mM) Reference: P037-01 These products are high-purity single-component dUTP/dATP/dTTP/dCTP/dGTP, each at a concentration of 100 mM, which can be used for PCR, real-time PCR, RT-PCR, primer extension reaction, DNA sequencing, DNA labeling and other conventional reactions used in molecular biology.Application:
Heat-labile UDG Reference: P051-01 UDG (Uracil-DNA Glycosylase, uracil-DNA glycosylase) can catalyze the hydrolysis of dU-containing DNA single-stranded or double-stranded uracil base and sugar phosphate backbone N-glycosidic bond, release free uracil, thereby The resulting abasic sites are easily broken by hydrolysis. Heat-labile UDG is derived from psychrophilic marine bacteria and is sensitive to high temperature. It can irreversibly inactivate enzymes above 50℃. It is suitable for PCR/QPCR and RT-PCR/RT-QPCR systems.Application: 55°C 10 min rapid inactivation; Compatible with common PCR reaction systems
Heat-labile UDG Reference: P051-02 UDG (Uracil-DNA Glycosylase, uracil-DNA glycosylase) can catalyze the hydrolysis of dU-containing DNA single-stranded or double-stranded uracil base and sugar phosphate backbone N-glycosidic bond, release free uracil, thereby The resulting abasic sites are easily broken by hydrolysis. Heat-labile UDG is derived from psychrophilic marine bacteria and is sensitive to high temperature. It can irreversibly inactivate enzymes above 50℃. It is suitable for PCR/QPCR and RT-PCR/RT-QPCR systems.Application: 55°C 10 min rapid inactivation; Compatible with common PCR reaction systems
E.coli UDG Reference: P061-01 E. coli uracil-DNA glycosylase (UDG) catalyzes the release of uracil from uracil-containing DNA. UDG can effectively hydrolyze uracil on single-stranded or double-stranded DNA, but cannot hydrolyze uracil from oligonucleotides of 6 bases or less.Application: After strict quality control
E.coli UDG Reference: P061-02 E. coli uracil-DNA glycosylase (UDG) catalyzes the release of uracil from uracil-containing DNA. UDG can effectively hydrolyze uracil on single-stranded or double-stranded DNA, but cannot hydrolyze uracil from oligonucleotides of 6 bases or less.Application: After strict quality control
Murine RNase Inhibitor Reference: R301-01 Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript® II Reverse Transcriptase, HiScript® Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).Application: The enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions; High-purity E. coli soluble expression, no RNase residue, compatible with RT-PCR/qPCR system
Murine RNase Inhibitor Reference: R301-02 Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript® II Reverse Transcriptase, HiScript® Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).Application: The enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions; High-purity E. coli soluble expression, no RNase residue, compatible with RT-PCR/qPCR system
Murine RNase Inhibitor Reference: R301-03 Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript® II Reverse Transcriptase, HiScript® Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).Application: The enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions; High-purity E. coli soluble expression, no RNase residue, compatible with RT-PCR/qPCR system
RoomTemp Sample Lysis Kit Reference: P073-01 RoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer, among which Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can be fully released The genomic DNA in the cell, Stabilizing Buffer is added with protective proteins and stabilizing factors, which can eliminate the inhibitory effect of the inhibitor in the lysed sample on the downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include Fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, sodium heparin, etc.). Genomic DNA can be released from whole blood samples after 3 minutes of lysis at room temperature. The lysed DNA solution can be directly used as a template ，Apply to Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification, etc. It can achieve the same effect as traditional genome extraction methods without complicated extraction operations. In addition to blood, this kit can also Compatible with pyrolysis FTA blood cards, oral swabs, plant tissues and other samples. Application: Simple operation: 3 minutes at room temperature, no need for complex template extraction. High lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome. Wide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.
RoomTemp Sample Lysis Kit Reference: P073-02 RoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer. Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can fully release the genomic DNA in the cell. Stabilizing Buffer adds protective proteins and stabilizing factors. Eliminate the inhibitory effect of inhibitors in the lysed sample on downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, heparin sodium, etc.). After lysis at room temperature for 3 minutes, genomic DNA can be released from the whole blood sample. The lysed DNA solution can be directly used as a template for SNP detection by Taqman ® probe method, qPCR probe method quantification, PCR amplification, etc. Without complicated extraction operations, it can achieve the same effect as traditional genome extraction methods. In addition to blood, this kit is also compatible with pyrolysis FTA blood cards, buccal swabs, plant tissues and other samples.Application: Simple operation: 3 minutes at room temperature, no need for complex template extraction. High lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome. Wide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.
RoomTemp Sample Lysis Kit Reference: P073-03 RoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer. Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can fully release the genomic DNA in the cell. Stabilizing Buffer adds protective proteins and stabilizing factors. Eliminate the inhibitory effect of inhibitors in the lysed sample on downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, heparin sodium, etc.). After lysis at room temperature for 3 minutes, genomic DNA can be released from the whole blood sample. The lysed DNA solution can be directly used as a template for SNP detection by Taqman ® probe method, qPCR probe method quantification, PCR amplification, etc. Without complicated extraction operations, it can achieve the same effect as traditional genome extraction methods. In addition to blood, this kit is also compatible with pyrolysis FTA blood cards, buccal swabs, plant tissues and other samples.Application: Simple operation: 3 minutes at room temperature, no need for complex template extraction. High lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome. Wide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.
RNA Keeper Tissue Stabilizer Reference: R501-01 RNA Keeper Tissue Stabilizer is a non-toxic solution that can quickly penetrate into tissues, inactivate endogenous RNase, immediately stabilize and protect the integrity of RNA. Fresh tissue samples do not need liquid nitrogen quick freezing, as long as they are immersed in RNA Keeper Tissue Stabilizer, they can be stored at 37°C for 1 day, room temperature for 1 week, 4°C for 4 weeks, or long-term storage at -20°C/-80°C, and repeated Freezing and thawing will not significantly affect the integrity of RNA. The samples stored in the RNA Keeper Tissue Stabilizer can be directly used to extract RNA using RNA isolater total RNA extraction reagent (R401-01) or spin column method. RNA Keeper Tissue Stabilizer can be used for the preservation of brain, heart, liver, pancreas, kidney, spleen, testis, muscle and other tissues.Application: Ultra-high amplification specificity: AceTaq® DNA Polymerase based on chemical hot-start, completely blocked enzyme activity before 95°C, and equipped with a patented specific promotion factor Exactor, which makes amplification more specific;