ClonExpress Ultra One Step Cloning Kit V2 Reference: C116-02 The ClonExpress technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning.ClonExpress Ultra One Step Cloning Kit V2 is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.
dNTP Mix (2.5 mM each) Reference: P032-01 This product is an equimolar mixture of high purity dATP, dGTP, dCTP, and dTTP, which can be directly used in PCR, RT (reverse transcription) and one-step RT-PCR experiments.Application: HPLC purity ≥99%; Strict quality control to ensure batch stability
E.Z.N.A.® Fungal DNA Mini Kit Reference: D3390-02 E.Z.N.A.® Fungal DNA Mini Kit allows for the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of fungal species and tissues. Up to 200 mg of wet tissue(or up to 50 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of Omega's HiBind® matrix with the speed and versatility of spin columns to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. There are no organic extractions, thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
Ultra-Universal TOPO Cloning Kit Reference: C603-01 Ultra-Universal TOPO Cloning Kit is the fourth-generation TOPO cloning kit. The kit uses upgraded Topoisomerase with the optimal buffer and has higher enzyme activity. The addition of the second-generation blunting factor and vector self-ligation inhibitor makes this product compatible with both TA cloning and blunt-end cloning, and increases the positive rate of cloning.Features - Upgraded version: 4th Generation topoisomerase; higher positive rate and colony counts- Fast: Cloning of either sticky-end or blunt-end DNA in only 5 minutes- Efficient: High efficiency cloning and positive rate>95%- Vector self-ligation inhibitor factor: Effectively prevent vector self-ligation and reduce false positives
dNTP Mix (2.5 mM each) Reference: P032-02 This product is an equimolar mixture of high purity dATP, dGTP, dCTP, and dTTP, which can be directly used in PCR, RT (reverse transcription) and one-step RT-PCR experiments.Application: HPLC purity ≥99%; Strict quality control to ensure batch stability
E.Z.N.A.® Blood DNA Mini Kit Reference: D3392-00 The E.Z.N.A.® Blood DNA Kit provides rapid total DNA isolation from 1-250 µL of fresh and frozen anticoagulated whole blood. The E.Z.N.A.® Blood DNA Kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® Blood DNA method is ready for applications such as PCR, Southern blotting, or restriction enzyme digestion.
Ultra-Universal TOPO Cloning Kit Reference: C603-02 Ultra-Universal TOPO Cloning Kit is the fourth-generation TOPO cloning kit. The kit uses upgraded Topoisomerase with the optimal buffer and has higher enzyme activity. The addition of the second-generation blunting factor and vector self-ligation inhibitor makes this product compatible with both TA cloning and blunt-end cloning, and increases the positive rate of cloning.Features - Upgraded version: 4th Generation topoisomerase; higher positive rate and colony counts- Fast: Cloning of either sticky-end or blunt-end DNA in only 5 minutes- Efficient: High efficiency cloning and positive rate>95%- Vector self-ligation inhibitor factor: Effectively prevent vector self-ligation and reduce false positives
dUTP (100 mM) Reference: P033-01 These products are high-purity single-component dUTP/dATP/dTTP/dCTP/dGTP, each at a concentration of 100 mM, which can be used for PCR, real-time PCR, RT-PCR, primer extension reaction, DNA sequencing, DNA labeling and other conventional reactions used in molecular biology.Application:
E.Z.N.A.® Blood DNA Mini Kit Reference: D3392-01 The E.Z.N.A.® Blood DNA Kit provides rapid total DNA isolation from 1-250 µL of fresh and frozen anticoagulated whole blood. The E.Z.N.A.® Blood DNA Kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® Blood DNA method is ready for applications such as PCR, Southern blotting, or restriction enzyme digestion.
ClonExpress Ultra One Step Cloning Kit V3 Reference: C117-01 ClonExpress Ultra One Step Cloning Kit V3 utilizes the latest optimized technology, enabling highly efficient homologous recombination of 1 - 11 DNA fragments. The newly optimized 2 × CE Mix V3 not only ensures a high cloning success rate for complicated situations such as large vectors and GC-rich/poor homologous arms, but also significantly enhances compatibility with amounts of DNA input. This greatly solves the problem of reduced cloning efficiency caused by low DNA fragment concentration.FeaturesRobust performance: 1 - 11 fragments assembly; the positive rate is > 95%High Efficiency: 5 - 30 min rapid recombination; high clones and positivity rateSpecial optimization: Maximum compatible with 15 - 35 bp overlaps, not only 15 - 20 bpBroad Compatibility: Suitable for ultra-low input, GC-rich/poor overlaps, and low-purity samples
dATP (100 mM) Reference: P034-01 These products are high-purity single-component dUTP/dATP/dTTP/dCTP/dGTP, each at a concentration of 100 mM, which can be used for PCR, real-time PCR, RT-PCR, primer extension reaction, DNA sequencing, DNA labeling and other conventional reactions used in molecular biology.Application:
E.Z.N.A.® Blood DNA Mini Kit Reference: D3392-02 The E.Z.N.A.® Blood DNA Kit provides rapid total DNA isolation from 1-250 µL of fresh and frozen anticoagulated whole blood. The E.Z.N.A.® Blood DNA Kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® Blood DNA method is ready for applications such as PCR, Southern blotting, or restriction enzyme digestion.