FreeZol Reagent Reference: R711-01 FreeZol Reagent is widely applicable to the extraction of total RNA from cultured cells, animal tissues, and simple plant tissues. Compared with the conventional Trizol method, this product features a simple procedure that can be performed at room temperature with no need to use chloroform for phase separation. In addition, this product ensures the integrity and purity of the extracted RNA by precipitating proteins, DNA, polysaccharides and other impurities in the organic phase while retaining RNA in the upper aqueous phase. The whole procedure can be completed in 1 h. The obtained total RNA can be used directly for RT-PCR, qRT-PCR, Northern blot, Dot blot, in vitro translation, NGS, and other molecular biology experiments.Features Safe and low in toxicity: No need for toxic and harmful reagents such as chloroform and β-mercaptoethanolEase to use: RNA extraction in a monophasic system, no phase separation, all operations at room temperatureHigh RNA quality: High purity and integrity of RNA at a yield comparable to the traditional Trizol method
FastPure Universal Plant Total RNA Isolation Kit Reference: RC411-01 FastPure Universal Plant Total RNA Isolation Kit can be used to quickly extract total RNA from plant tissues. The kit includes two solution systems designed for simple RNA extraction from a variety of plant tissues (wheat, rice, corn, Arabidopsis thaliana, tobacco, rape, etc.), polysaccharide and polyphenol rich plant tissues (cotton leaf, soybean leaf, pine needle, ginkgo leaf, fig leaf, gardenia leaf, wheat seed, corn seed, red bean seed, potato, sweet potato, soybean seed, sesame seed, peanut seeds, rapeseed, etc.), fruit pulp (watermelon, apple, peach, pear, banana, mango, etc.), and fungi (shiitake, button mushroom, oyster mushroom, Neurospora crassa, etc.). The kit is based on silica column purification technology and does not involve toxic reagents (e.g., phenol or chloroform and β-mercaptoethanol) or time-consuming alcohol precipitation. The whole extraction process takes only 11 min. FastPure gDNA-Filter Columns III in the kit effectively remove impurities and gDNA. FastPure RNA Columns V efficiently bind to RNA and, paired with optimized buffers, yield high-purity total RNA with minimal gDNA residue and no protein or other impurities that can be used for a range of downstream experiments, including RT-PCR, real-time PCR, RNA library preparation, microarray analysis, Northern blot, dot blot, and molecular cloning.