5min Universal Ligation Mix Reference: C311-02 5minTM Universal Ligation Mix is a ready-to-use 2× premix that mixes enzyme and Buffer. It contains T4 DNA ligase, which catalyzes the formation of adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA to form phosphate diphosphates. Ester bond. This kit is not only suitable for sticky end ligation, but also compatible with DNA ligation with blunt ends and single-base overhanging ends. The optimized connection enhancer and reaction buffer make the reaction more efficient and convenient. The reaction can be quickly connected at 25°C for 5 minutes. The ligation product can directly transform a variety of chemically competent cells.Application: Compatible: compatible with sticky ends, smooth ends, TA connection, Linker or Adapter connection; Fast: 25℃, 5 minutes can be connected quickly; High efficiency: High cloning efficiency.
E.Z.N.A.® Total RNA Kit II Reference: R6934-01 The E.Z.N.A.® Total RNA Kit II is designed for isolating total cellular RNA from tissues rich in fat, such as brain adipose tissues. However, this kit can also be used for the isolation of total RNA from other types of tissues including cultured eukaryotic cells, animal tissues, or bacteria. The E.Z.N.A.® Total RNA Kit II uses the reversible binding properties of HiBind® matrix, a new silica-based material. By combining the high lysis efficiency of RNA-Solv® Reagent with Omega Bio-tek's innovative HiBind® technology, this kit can extract total cellular RNA from all types of animal or human tissues including fatty tissues such as brain and adipose tissue. A specifically formulated high salt buffer system allows more than 100 µg RNA molecules greater than 200 bases to bind to the matrix. Cells or tissues are first homogenized with RNA-Solv® Reagent that inactivates RNases. After adding chloroform, the homogenate is separated into an aqueous and organic phase by centrifugation. The aqueous phase which contains the RNA is adjusted with ethanol and applied to the HiBind® RNA Mini Column to which total RNA binds, while cellular debris and other contaminants are washed away. High-quality RNA is eluted in Nuclease-Free Water.
5min TA/Blunt-Zero Cloning Kit Reference: C601-01 This product is the second-generation TOPO cloning kit, which contains the second-generation Topoisomerase, a vector containing the suicide gene ccdB, and a blunt-end factor. Using the second-generation Topoisomerase, with the most suitable buffer, it has higher activity and further improves the cloning efficiency. The efficient connection is completed in 5 minutes at room temperature. Using a vector containing the suicide gene ccdB, when the insert is successfully connected to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thus achieving a "Zero" background. The addition of blunt-end factor makes this product compatible with TA cloning and blunt-end cloning.Application: Compatible: Compatible with TA cloning and blunt end cloning at the same time; Fast: add fragments and react for 5 minutes; High efficiency: high cloning efficiency and positive rate>95%.
PCR Enhancer Reference: P021-01 During conventional PCR analysis, DNA fragments with high GC content are often difficult to amplify due to their stable secondary structure. Under ordinary PCR reaction conditions, DNA polymerase is difficult or even impossible to amplify. It can intervene in the secondary structure of high GC content DNA. PCR Enhancer is a mixed additive composed of multiple components, which can effectively reduce the melting temperature of high GC templates and templates with complex secondary structures, and is compatible with almost all DNA polymerase amplification systems. When the optimized PCR program cannot effectively amplify the target fragment, adding PCR Enhancer can often get unexpected results. Application: Increase amplification stability; Improve amplification efficiency
E.Z.N.A.® Total RNA Kit II Reference: R6934-02 The E.Z.N.A.® Total RNA Kit II is designed for isolating total cellular RNA from tissues rich in fat, such as brain adipose tissues. However, this kit can also be used for the isolation of total RNA from other types of tissues including cultured eukaryotic cells, animal tissues, or bacteria. The E.Z.N.A.® Total RNA Kit II uses the reversible binding properties of HiBind® matrix, a new silica-based material. By combining the high lysis efficiency of RNA-Solv® Reagent with Omega Bio-tek's innovative HiBind® technology, this kit can extract total cellular RNA from all types of animal or human tissues including fatty tissues such as brain and adipose tissue. A specifically formulated high salt buffer system allows more than 100 µg RNA molecules greater than 200 bases to bind to the matrix. Cells or tissues are first homogenized with RNA-Solv® Reagent that inactivates RNases. After adding chloroform, the homogenate is separated into an aqueous and organic phase by centrifugation. The aqueous phase which contains the RNA is adjusted with ethanol and applied to the HiBind® RNA Mini Column to which total RNA binds, while cellular debris and other contaminants are washed away. High-quality RNA is eluted in Nuclease-Free Water.
5min TA/Blunt-Zero Cloning Kit Reference: C601-02 This product is the second-generation TOPO cloning kit, which contains the second-generation Topoisomerase, a vector containing the suicide gene ccdB, and a blunt-end factor. Using the second-generation Topoisomerase, with the most suitable buffer, it has higher activity and further improves the cloning efficiency. The efficient connection is completed in 5 minutes at room temperature. Using a vector containing the suicide gene ccdB, when the insert is successfully connected to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thus achieving a "Zero" background. The addition of blunt-end factor makes this product compatible with TA cloning and blunt-end cloning.Application: Compatible: Compatible with TA cloning and blunt end cloning at the same time; Fast: add fragments and react for 5 minutes; High efficiency: high cloning efficiency and positive rate>95%.
dNTP Mix (10 mM each) Reference: P031-01 This product is an equimolar mixture of high purity dATP, dGTP, dCTP, and dTTP, which can be directly used in PCR, RT (reverse transcription) and one-step RT-PCR experiments.Application: HPLC purity ≥99%; Strict quality control to ensure batch stability
E.Z.N.A.® Bacterial RNA Kit Reference: R6950-00 The E.Z.N.A.® Bacterial RNA Kit is designed for the isolation of high-quality total RNA from a variety of bacterial strains. Up to 1 x 10^9 log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridization techniques.
ClonExpress Ultra One Step Cloning Kit V2 Reference: C116-01 The ClonExpress technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning.ClonExpress Ultra One Step Cloning Kit V2 is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.
dNTP Mix (10 mM each) Reference: P031-02 This product is an equimolar mixture of high purity dATP, dGTP, dCTP, and dTTP, which can be directly used in PCR, RT (reverse transcription) and one-step RT-PCR experiments.Application: HPLC purity ≥99%; Strict quality control to ensure batch stability
E.Z.N.A.® Bacterial RNA Kit Reference: R6950-01 The E.Z.N.A.® Bacterial RNA Kit is designed for the isolation of high-quality total RNA from a variety of bacterial strains. Up to 1 x 10^9 log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridization techniques.