E.Z.N.A.® FFPE RNA Kit Reference: R6954-00 The E.Z.N.A.® FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscle tissue. The sample is first processed in a special buffer by heating to deactivate RNases and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then applied to a HiBind RNA column to capture RNA. After two quick wash steps, purified RNA is eluted with water. While E.Z.N.A.® FFPE RNA Kit is optimized to minimize the effect of formaldehyde modification, it is not recommended to use the RNA purified from this kit for downstream applications that require full-length RNA.
dNTP Mix (2.5 mM each) Reference: P032-02 This product is an equimolar mixture of high purity dATP, dGTP, dCTP, and dTTP, which can be directly used in PCR, RT (reverse transcription) and one-step RT-PCR experiments.Application: HPLC purity ≥99%; Strict quality control to ensure batch stability
E.Z.N.A.® FFPE RNA Kit Reference: R6954-01 The E.Z.N.A.® FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscle tissue. The sample is first processed in a special buffer by heating to deactivate RNases and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then applied to a HiBind RNA column to capture RNA. After two quick wash steps, purified RNA is eluted with water. While E.Z.N.A.® FFPE RNA Kit is optimized to minimize the effect of formaldehyde modification, it is not recommended to use the RNA purified from this kit for downstream applications that require full-length RNA.
dUTP (100 mM) Reference: P033-01 These products are high-purity single-component dUTP/dATP/dTTP/dCTP/dGTP, each at a concentration of 100 mM, which can be used for PCR, real-time PCR, RT-PCR, primer extension reaction, DNA sequencing, DNA labeling and other conventional reactions used in molecular biology.Application:
E.Z.N.A.® Micro RNA Kit Reference: R7034-01 The E.Z.N.A.® Micro RNA Kit combines the reversible binding properties of the HiBind® matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications. The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is applied to a HiBind® RNA column where large RNA binds. The flow-through is retained and applied to a HiBind® Micro RNA column to bind small RNA.
dATP (100 mM) Reference: P034-01 These products are high-purity single-component dUTP/dATP/dTTP/dCTP/dGTP, each at a concentration of 100 mM, which can be used for PCR, real-time PCR, RT-PCR, primer extension reaction, DNA sequencing, DNA labeling and other conventional reactions used in molecular biology.Application:
HiBind® RNA Mini Columns Reference: RNACOL-01 HiBind® RNA Spin Columns, V-Spin are mini spin columns with a cap that can be used either in microcentrifuges or on vacuum manifolds for purification of RNA.
dTTP (100 mM) Reference: P035-01 These products are high-purity single-component dUTP/dATP/dTTP/dCTP/dGTP, each at a concentration of 100 mM, which can be used for PCR, real-time PCR, RT-PCR, primer extension reaction, DNA sequencing, DNA labeling and other conventional reactions used in molecular biology.Application:
HiBind® RNA Mini Columns Reference: RNACOL-02 HiBind® RNA Spin Columns, V-Spin are mini spin columns with a cap that can be used either in microcentrifuges or on vacuum manifolds for purification of RNA.
RNA isolater Total RNA Extraction Reagent Reference: R401-01 RNA isolater Total RNA Extraction Reagent, based on guanidine isothiocyanate and phenol, has a strong lysis ability, which can lyse cells and tissue samples in a short time to ensure the integrity of RNA. This product is widely used for culturing cells, animal tissues, microorganisms, and plant tissues with less secondary metabolism, such as seedlings and young leaves. The sample can be fully lysed in RNA isolater. After centrifugation with chloroform, the solution will form a supernatant layer, an intermediate layer and an organic layer (bright red lower layer). RNA is distributed in the upper aqueous phase. After the supernatant layer is collected, the Total RNA can be recovered by propanol precipitation.Application: Based on guanidine isothiocyanate and phenol, strong cracking ability; The optimized lysate formula has a wide range of sample adaptability.
dCTP (100 mM) Reference: P036-01 These products are high-purity single-component dUTP/dATP/dTTP/dCTP/dGTP, each at a concentration of 100 mM, which can be used for PCR, real-time PCR, RT-PCR, primer extension reaction, DNA sequencing, DNA labeling and other conventional reactions used in molecular biology.Application:
RNA-easy Isolation Reagent Reference: R701-01 RNA-easy Isolation Reagent is widely used to extract Total RNA and Small RNA from samples of various animal tissues, plant materials, cultured cells and microorganisms. Compared with the traditional Trizol extraction method, this product is simple to use, does not require the use of chloroform for stratification, and the whole process can be performed at room temperature; in addition, this product effectively inhibits the activity of RNase (RNase) while simultaneously removing protein and DNA. , Polysaccharides and other impurities precipitate to the bottom of the tube to achieve single-phase extraction and effectively ensure the integrity and purity of RNA. The entire extraction process can be completed within 50 minutes using this product. The extracted RNA can be directly used in various molecular biology such as cDNA cloning, qRT-PCR detection, mRNA purification, in vitro translation, Northern blotting hybridization, high-throughput sequencing, etc. experiment.Application: Broad platform compatibility: The addition of universal ROX to miRNA Universal SYBR® qPCR Master Mix enables mix to be compatible with qPCR instruments on different platforms without the need to adjust ROX concentration.