E.Z.N.A.® Blood DNA Maxi Kit Reference: D2492-03 The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large-scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 20 mL whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.
ConviFlex™ RT-Taq Mix Reference: 192-0100 1-Step Master Mix designed for fast, highly sensitive real-time RT-PCR. The freeze-dried formulation provides enhanced detection of both RNA and DNA templates, even with low target input.
Mut Express II Fast Mutagenesis Kit V2 Reference: C214-01 Mut Express® II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, the target plasmid amplification product is digested with DpnI, ClonExpress® recombination and circularized, and then directly transformed to complete the site-directed mutation. The kit consists of Phanta® Max Super-Fidelity DNA Polymerase amplification module and ClonExpress® rapid cloning module. The ultra-high fidelity of Phanta® MaxSuper-Fidelity DNA Polymerase significantly reduces the possibility of introducing new mutations during the amplification process. The ClonExpress® rapid cloning system uses efficient homologous recombination to replace the traditional annealing loop reaction. Highly optimized reaction buffers, fast operating procedures and extremely high success rate make Mut Express® II Fast Mutagenesis Kit V2 the first choice for DNA site-directed mutagenesis.Application: Phanta® Max Super-Fidelity DNA Polymerase high-fidelity enzyme provides PCR reactions with ultra-high fidelity; The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template; The amplified product can be directly used in the recombination reaction after being digested by DpnI; Site-directed mutagenesis can be performed on a single site or two discrete sites (more than 50 bp apart) on the target plasmid at the same time
E.Z.N.A.® HP Fungal DNA Kit Reference: D3195-00 The E.Z.N.A.® High Performance (HP) Fungal DNA Kit is designed for efficient recovery of genomic DNA from fresh and dried fungal tissue samples rich in polysaccharides or having lower DNA content. Up to 100 mg wet tissue(or 30 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin-column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.
ConviFlex™ RT-Taq Mix Reference: 192-0250 1-Step Master Mix designed for fast, highly sensitive real-time RT-PCR. The freeze-dried formulation provides enhanced detection of both RNA and DNA templates, even with low target input.
Mut Express II Fast Mutagenesis Kit V2 Reference: C214-02 Mut Express® II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, the target plasmid amplification product is digested by DpnI, ClonExpress® recombination and circularized, and then directly transformed to complete the site-directed mutation. The kit consists of Phanta® Max Super-Fidelity DNA Polymerase amplification module and ClonExpress® rapid cloning module. The ultra-high fidelity of Phanta® MaxSuper-Fidelity DNA Polymerase significantly reduces the possibility of introducing new mutations during the amplification process. The ClonExpress® rapid cloning system uses efficient homologous recombination to replace the traditional annealing loop reaction. Highly optimized reaction buffers, fast operating procedures and extremely high success rate make Mut Express® II Fast Mutagenesis Kit V2 the first choice for DNA site-directed mutagenesis.Application: Phanta® Max Super-Fidelity DNA Polymerase high-fidelity enzyme provides PCR reactions with ultra-high fidelity; The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template; The amplified product can be directly used in the recombination reaction after being digested by DpnI; Site-directed mutagenesis can be performed on a single site or two discrete sites (more than 50 bp apart) on the target plasmid at the same time
E.Z.N.A.® HP Fungal DNA Kit Reference: D3195-01 The E.Z.N.A.® High Performance (HP) Fungal DNA Kit is designed for efficient recovery of genomic DNA from fresh and dried fungal tissue samples rich in polysaccharides or having lower DNA content. Up to 100 mg wet tissue(or 30 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin-column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.
Mut Express MultiS Fast Mutagenesis Kit V2 Reference: C215-01 Mut Express® MultiS Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, three to five discrete sites on the target plasmid can be introduced at one time. The kit consists of two The ultra-high fidelity of Phanta® Max significantly reduces the possibility of introducing new mutations during the amplification process. Its excellent long-fragment amplification capability is widely applicable to the amplification of any plasmid with a length less than 20 kb. ClonExpress® rapid cloning System utilizes efficient homologous recombination. The reaction replaces the traditional annealing reaction. Therefore, use Mut Express® MultiS site-directed mutagenesis kit for DNA site-directed In the case of mutation, the primer design is more flexible, and the amplification reaction is no longer performed in a linear manner, and the template usage is extremely low, which is beneficial to the original Complete degradation of the basic template. The kit is equipped with Exnase® MultiS, a recombinase optimized for multi-base site-directed mutagenesis, And if the amplified product is specific, the DpnI digested product can be used directly in the recombination reaction without DNA purification. Highly optimized reaction buffer Mut Express® MuiltiS Fast Mutagenesis Kit V2 has become the preferred kit for DNA multipoint mutations. Application: Phanta® Max Super-Fidelity DNA Polymerase provides high-fidelity PCR with the lowest mutation rate; Phanta® Max Super-Fidelity DNA Polymerase has excellent long fragment amplification ability, which is widely applicable to the amplification of any plasmid within 20 kb; The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template; ClonExpress® rapid cloning system efficiently circularizes PCR products; DpnI eliminates original template contamination Site-directed mutagenesis of three to five discrete sites (more than 50 bp apart) on the target plasmid can be achieved in one step
E.Z.N.A.® Bacterial DNA Kit Reference: D3350-00 The E.Z.N.A.® Bacterial DNA Kit allows the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of gram-positive and negative bacterial species. This kit uses an optimized lysis condition and up to 1 x 10^9 bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or Elution Buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
Mut Express MultiS Fast Mutagenesis Kit V2 Reference: C215-02 Mut Express® MultiS Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, three to five discrete sites on the target plasmid can be introduced at one time. The kit consists of two The ultra-high fidelity of Phanta® Max significantly reduces the possibility of introducing new mutations during the amplification process. Its excellent long-fragment amplification capability is widely applicable to the amplification of any plasmid with a length less than 20 kb. ClonExpress® rapid cloning System utilizes efficient homologous recombination. The reaction replaces the traditional annealing reaction. Therefore, use Mut Express® MultiS site-directed mutagenesis kit for DNA site-directed In the case of mutation, the primer design is more flexible, and the amplification reaction is no longer performed in a linear manner, and the template usage is extremely low, which is beneficial to the original. Complete degradation of the basic template. The kit is equipped with Exnase® MultiS, a recombinase optimized for multi-base site-directed mutagenesis, And if the amplified product is specific, the DpnI digested product can be used directly in the recombination reaction without DNA purification. Highly optimized reaction buffer Mut Express® MuiltiS Fast Mutagenesis Kit V2 has become the preferred kit for DNA multipoint mutations. Application: Phanta® Max Super-Fidelity DNA Polymerase provides high-fidelity PCR with the lowest mutation rate; Phanta® Max Super-Fidelity DNA Polymerase has excellent long fragment amplification ability, which is widely applicable to the amplification of any plasmid within 20 kb; The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template; ClonExpress® rapid cloning system efficiently circularizes PCR products; DpnI eliminates original template contamination Site-directed mutagenesis of three to five discrete sites (more than 50 bp apart) on the target plasmid can be achieved in one step
E.Z.N.A.® Bacterial DNA Kit Reference: D3350-01 The E.Z.N.A.® Bacterial DNA Kit allows the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of gram-positive and negative bacterial species. This kit uses an optimized lysis condition and up to 1 x 10^9 bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or Elution Buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
T4 DNA Ligase Reference: C301-01 T4 DNA Ligase can catalyze the formation of phosphodiester bonds between the 5'phosphate end and 3'hydroxyl end of the adjacent nucleic acid at the blunt or sticky end of dsDNA. It can also catalyze the connection between RNA and ssDNA or RNA strands in the double strand, but it cannot catalyze the entire single Chain nucleotide connection. Suitable for nucleic acid operations such as labeling RNA 3'-end, circularizing RNA and DNA oligonucleotides and cloning cDNA.Application: Purity is greater than 99%, no exogenous nuclease activity remains; The classic buffer composition allows the ligation reaction to proceed efficiently