Trident 10X PBST Reference: GTX30977 This buffer is supplied as 10X PBS solution containing 1% Tween. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc. This buffer does not contain any sodium azide or mercury preservative and can be used for dilution of all antibodies, including peroxidase. This buffer does not contain any proteins.
RPMI 1640, with L-Glutamine, with 25 mM HEPES Reference: RPMI-HA RPMI 1640 medium is widely suitable for supporting growth of many cell types. Compared to other media, it has a unique formulation comprising glutathione and high concentrations of vitamins.
RPMI 1640, w/o L-Glutamine, with 25 mM HEPES Reference: RPMI-HXA RPMI 1640 medium is widely suitable for supporting growth of many cell types. Compared to other media, it has a unique formulation comprising glutathione and high concentrations of vitamins.
Citrate buffer, 10X pH6.0 Reference: GTX30936 This buffer is intended for heat-induced epitope retrieval (HIER) of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC, commonly used fixatives (like formalin) mask tissue antigens through cross-linking, often resulting in poor or no staining. The use of this buffer on FFPE tissue sections improves antibody accessibility to tissue antigens.
RPMI 1640, with Stable Glutamine Reference: RPMI-STA RPMI 1640 medium is widely suitable for supporting growth of many cell types. Compared to other media, it has a unique formulation comprising glutathione and high concentrations of vitamins.
EDTA buffer, 10X pH 8.0 Reference: GTX30937 This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
RPMI 1640, w/o L-Glutamine Reference: RPMI-XA RPMI 1640 medium is widely suitable for supporting growth of many cell types. Compared to other media, it has a unique formulation comprising glutathione and high concentrations of vitamins.