Exo-spin mini-HD columns Reference: EX05-100 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Leibovitz’s L-15 Medium, w/o L-Glutamine Reference: L15-XA Leibovitz's L-15 Medium was developed for carbon dioxide free systems. It is suitable for the cultivation of tumor and embryonic cells as well as HeLa and Hep-2.
Newborn Calf Serum, Heat Inactivated Reference: NCS-HI-1A Newborn Calf Serum (NBCS) is collected from 3 to 10 day old calves and can be used as a high-grade replacement for Fetal Bovine Serum.
1X PBS (10L) Buffer Powder Packets - 1 PKG Reference: P1121-1PKG Our Phosphate Buffered Saline (PBS), pH 7.2–7.6, is a highly reliable and ready-to-use biological buffer essential for molecular biology and cell culture applications. Each foil pouch contains a precisely measured, high-quality powder blend that dissolves to create a 10L volume of a 1X working solution. This eliminates the need for complex calculations, measurements, and pH adjustments, saving you valuable lab time. Applications Use our 1X PBS as a gentle, isotonic wash solution in a wide range of assays: - ELISA and Western Blotting (Protein Applications) - Cell Culture (Sterile Filtration Recommended; Washing and Resuspension) - Immunohistochemistry (IHC) - Electrophoresis (e.g., as a sample diluent or wash) - Resuspension and Sonication of cells
FuGENE® SI Transfection Reagent Reference: SI-5000 Harnessing the same gentle reliability as our market-leading DNA Transfection Reagents, we are proud to introduce to you FuGENE® SI, an innovative RNA Transfection reagent engineered for the delivery of siRNA. FuGENE® SI is a 100% synthetic, multi-component transfection reagent designed for the delivery of RNA molecules into eukaryotic cell lines. FuGENE® SI allows researchers to obtain maximum efficiency knockdowns while being more gentle on cells than competitor’s reagents. Request a sample today for your RNA delivery needs and See the Unseen® with FuGENE® SI.
Exo-spin 96 Reference: EX07-96 Exo-spin™ 96 provides proven size exclusion chromatography (SEC) mini-column (EX01) technology in a 96-well format, allowing up to 96 individual samples to be processed in less than 30 minutes. Sample volumes up to 0.5 ml can be purified.
Medium 199 with Earle’s Salts, with L-Glutamine Reference: M199-A Medium 199 (M199) was developed for the culture of primary explants. It is suitable for vaccine production, virology and other in vitro cell culture applications.
Newborn Calf Serum, Heat Inactivated Reference: NCS-HI-1B Newborn Calf Serum (NBCS) is collected from 3 to 10 day old calves and can be used as a high-grade replacement for Fetal Bovine Serum.
1X PBS (10L) Buffer Powder Packets - 5 PKG Reference: P364-4x1L-3 Our Phosphate Buffered Saline (PBS), pH 7.2–7.6, is a highly reliable and ready-to-use biological buffer essential for molecular biology and cell culture applications. Each foil pouch contains a precisely measured, high-quality powder blend that dissolves to create a 10L volume of a 1X working solution. This eliminates the need for complex calculations, measurements, and pH adjustments, saving you valuable lab time. Applications Use our 1X PBS as a gentle, isotonic wash solution in a wide range of assays: - ELISA and Western Blotting (Protein Applications) - Cell Culture (Sterile Filtration Recommended; Washing and Resuspension) - Immunohistochemistry (IHC) - Electrophoresis (e.g., as a sample diluent or wash) - Resuspension and Sonication of cells
DIVERSA FluoGreen Delivery Nanoparticles Reference: DIV000F1 These fluorescent nanoparticles are designed for efficient cellular uptake, a key aspect in cellular biology and drug delivery research. Ideal for live cell imaging and therapeutic applications, this reagent ensures rapid internalization in various cell lines, supporting studies in immunology, oncology, and gene therapy. This cell culture reagent also has an application as positive control for exosome research. Its compatibility with fluorescent microscopy, including confocal and flow cytometry, makes it an essential tool in labs focusing on cell internalization, cell tracking, nanomedicine, and development of advanced therapeutics and cell therapies. DIVERSA fluorescent nanoparticles are biocompatible, outperforming other approaches based on inorganic nanoparticles and quantum dots. DIVERSA can customized them with alternative fluorophores as Cy3, Cy5, Cy7; This makes specific cell population labeling possible to and understanding cell to cell interactions, the relation with the immune system, and in vivo fate, among other applications.
TRIFic CD9 Exosome Assay Reference: EX101 The TRIFic™ exosome detection assay delivers clear and consistent data quickly by providing quantitative data from purified or unpurified samples, including direct measurement of exosomes in plasma in a convenient 96-well format. It is similar to an ELISA assay, but the target is detected directly with a Europium label without any enzymatic reaction. TRIFic™ exosome detection assays are being made available for widely used exosome markers, allowing for identification of CD9+/CD81+/CD63+ - EVs.
McCoy’s 5A Medium, with L-Glutamine Reference: MCC-A McCoy's 5A Medium supports the growth of primary cells, established cell lines, and explants. It is also used for production of virus in primary cell cultures.