EDTA buffer, 10X pH 8.0 Reference: GTX30937 This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Trident RIPA Lysis Buffer Reference: GTX400005 RIPA lysis buffer is used to efficiently lyse most adherent and non-adherent mammalian cells. The extraction of cytosolic, membrane and nuclear proteins can be achieved by using GTX 400005 RIPA Lysis buffer. As the reagent would not adversely affect the antibodies and protein antigens, it often can be used for immunoprecipitation assay.
Blocking Buffer, PBS with 1% Casein Reference: GTX48880 GeneTex's Casein Blocking Buffers are ready-to-use, PBS or TBS solutions of purified casein protein for blocking steps in Western blotting, ELISA, immunohistochemistry and nucleic acid detection methods.These blocking buffers contains casein protein that is purified from milk by the Hammarsten method. The phosphate- and Tris-buffered saline formulations are used for blocking excess binding sites on membranes and microplates in antibody-based detection methods. Blocker Casein Buffers are 1% (w/v) casein, which corresponds to the optimal concentration for most applications.Features and Benefits: ‧ Purified casein – single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions ‧ Easy to use – 1% casein solutions are ready to use; can be diluted further as needed ‧ Flexible – available in PBS and TBS formulations to suit a variety of applications ‧ Safe – stable, thimerosal-free formulations