Sodium bicarbonate, 7.5%, (NAH) Reference: LK002069 7.5% Sodium bicarbonate concentrate (NaHCO3), used to buffer the diluted CMF-HBSS; 1 x 100 ml
0.15m, MOPS Buffer, (MOPS) Reference: LK002070 0.15 M MOPS, pH 7.5, 0.22 u filtered. Buffer concentrate used to buffer the constituted Leibovitz L-15 media in Hepatocyte Isolation System.
Papain Dissociation System Reference: LK003150 Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Use-tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
Papain Dissociation System Reference: LK003153 Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Use-tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
Papain Dissociation System, Without EBSS Reference: LK003160 Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).
Papain Dissociation System, Without EBSS Reference: LK003163 Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).
DNase Vial (D2) Reference: LK003170 A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).
DNase Vial (D2) Reference: LK003172 A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).
PDS Kit, Papain Vial Reference: LK003176 A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1 mM L-cysteine with 0.5 mM EDTA.
PDS Kit, Papain Vial Reference: LK003178 A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1 mM L-cysteine with 0.5 mM EDTA.
PDS Kit, Inhibitor Vial Reference: LK003182 Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and lyophilized in autoclaved vials to contain 10 mg/ml each upon reconstitution with 32 ml of EBSS.
PDS Kit, EBSS Vial Reference: LK003188 Earle's balanced salt solution (EBSS) as supplied in the Papain Dissociation System.