Collagenase Vial, NCIS Reference: LK003245 A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of HBSS or equivalent yields a solution of 300 units/ml of collagenase, Code: CLSPA. Suitable for cell isolation and culture applications.
L-15 Media Powder (L15NK) Reference: LK003250 Leibovitz L-15 media powder, a component of the NCIS kit. Reconstitute entire contents of pouch, QS to 1 liter with cell culture grade water, and 0.22 micron filter. Suitable for cell isolation and culture applications.
Cell Strainers (Falcon) Reference: LK003265 Cell strainers (Falcon), components of the NCIS kit. Suitable for removal of tissue debris in cell isolation applications.
Neonatal Cardiomyocyte Isolation System Reference: LK003300 Kit for performing five separate tissue dissociations each containing up to twelve hearts. Contains single use vials of purified collagenase and trypsin, CMF-HBSS, Leibovitz L-15 media and Falcon cell strainers along with a detailed protocol. The kit is use-tested by Worthington to assure performance.
Neonatal Cardiomyocyte Isolation System Reference: LK003303 Kit for performing five separate tissue dissociations each containing up to twelve hearts. Contains single use vials of purified collagenase and trypsin, CMF-HBSS, Leibovitz L-15 media and Falcon cell strainers along with a detailed protocol. The kit is use-tested by Worthington to assure performance.
Albumin, Nuclease-Free Reference: LS000290 Prepared by a method developed at Worthington. Some degradation products may be present. ≥90% of the material is intact BSA as determined by SDS PAGE. Tested for exonuclease, endonuclease, ribonuclease, and protease. An aqueous solution at neutral pH in 50% glycerol at 50 mg/ml.
Albumin, Nuclease-Free Reference: LS000291 Prepared by a method developed at Worthington. Some degradation products may be present. ≥90% of the material is intact BSA as determined by SDS PAGE. Tested for exonuclease, endonuclease, ribonuclease, and protease. An aqueous solution at neutral pH in 50% glycerol at 50 mg/ml.
Albumin, Nuclease-Free Reference: LS000292 Prepared by a method developed at Worthington. Some degradation products may be present. ≥90% of the material is intact BSA as determined by SDS PAGE. Tested for exonuclease, endonuclease, ribonuclease, and protease. An aqueous solution at neutral pH in 50% glycerol at 50 mg/ml.
Actin Reference: LS001041 Prepared by modification of the procedure of Spudich and Watt, J. Biol. Chem., 246, 4866 (1971). Purity checked by SDS-PAGE. A lyophilized powder.