Celase® GMP Collagenase Blend, Special Packaging Reference: 1235-PKG Celase® GMP is a proprietary, proteolytic enzyme blend produced, packaged and labeled by Cytori Therapeutics and distributed by WBC as supplied designed for efficient, gentle and reproducible in vitro dissociation of nucleated cells from adipose and other tissue. Produced using avian and mammalian tissue free raw materials, aseptic processes and sterile filtration under GMP guidelines to assure the lowest levels of impurities. A single vial containing both collagenase and a neutral protease can digest up to 280gm of adipose tissue. Special Packaging Required
Hepatocyte Isolation System Reference: LK002060 Kit for performing five separate adult rat liver perfusions. Contains five single use CLSH enzyme vials, five single use DNase vials, 10X CMF-Hank's Balanced Salt Solution, L-15 Media Powder, 0.15 M MOPS buffer,7.5% Sodium Bicarbonate Solution, and optimized protocol. The kit is use-tested by Worthington to assure performance.
Hank's Balanced Salt Solution 10X (HBSS10) Reference: LK002064 10X CMF-HBSS Concentrate, 1 bottle, 500 ml. Sterile calcium- and magnesium-free hank's Balanced Salt Solution (CMF-HBSS). The solution is used for washing and perfusing the liver prior to the addition of the dissociating enzyme solution. Store at 2-8°C.
Collagenase/Elastase Vial (CLSH) Reference: LK002066 Enzyme vial of HIS kit that contains ≥20,000 units of collagenase (Code: CLS-1) and 30 units of elastase (Code: ESL), filtered through 0.22 micron membrane and lyophilized.
Collagenase/Elastase Vial (CLSH) Reference: LK002067 Enzyme vial of HIS kit that contains ≥20,000 units of collagenase (Code: CLS-1) and 30 units of elastase (Code: ESL), filtered through 0.22 micron membrane and lyophilized.
Sodium bicarbonate, 7.5%, (NAH) Reference: LK002069 7.5% Sodium bicarbonate concentrate (NaHCO3), used to buffer the diluted CMF-HBSS; 1 x 100 ml
0.15m, MOPS Buffer, (MOPS) Reference: LK002070 0.15 M MOPS, pH 7.5, 0.22 u filtered. Buffer concentrate used to buffer the constituted Leibovitz L-15 media in Hepatocyte Isolation System.
Papain Dissociation System Reference: LK003150 Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Use-tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
Papain Dissociation System Reference: LK003153 Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Use-tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
Papain Dissociation System, Without EBSS Reference: LK003160 Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).
Papain Dissociation System, Without EBSS Reference: LK003163 Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).
DNase Vial (D2) Reference: LK003170 A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).