Exo-spin midi columns Reference: EX04-20 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Trypsin-EDTA (0.05 %) in DPBS (1x) Reference: TRY-1B Trypsin solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.
DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate Reference: DMEM-LPXA Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Mouse Serum Reference: MOU-1B Mouse Serum is obtained and pooled from normal mouse populations under strict conditions. It acts as a control or a blocking agent in binding assays.
Exo-spin mini-HD columns Reference: EX05-5 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Trypsin-EDTA (0.5 %) in DPBS (10x) Reference: TRY-1B10 Trypsin solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.
Ham’s F-12, with L-Glutamine Reference: HAM-12-A Ham's F-12 was developed for the cultivation of Chinese Hamster Ovary (CHO) cells. It is the medium of choice for supporting the growth of cells in serum-free conditions.
Newborn Calf Serum Reference: NCS-1A Newborn Calf Serum (NBCS) is collected from 3 to 10 day old calves and can be used as a high-grade replacement for Fetal Bovine Serum.
Exo-spin mini-HD columns Reference: EX05-20 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.