Category: Cell Culture

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  • Categories: Transfection
Reference: C-12219

Human Dermal Lymphatic Endothelial Cells (HDLEC) adult donor

Reference: HD-1000

FuGENE® HD is a 100% synthetic, multi-component, non-liposomal transfection reagent designed for the delivery of DNA into more challenging eukaryotic and insect cell lines.  FuGENE® HD interacts with nucleic acids & the cell’s membrane to provide efficient and safe entry into the cell. This mechanism allows users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells, and has successfully been used to transfect over 1000 cell lines. With a protocol that is straight-forward and quick, FuGENE® HD allows researchers and scientists to free up valuable lab time.

Reference: EX06-250

Exo-spin™ products provide a proven, rapid and reliable way of generating high-quality purified exosomes suitable for a range of research applications.

Reference: EX04-5

Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.

Reference: C-12221

Human Coronary Artery Endothelial Cells (HCAEC)

Reference: HD-5000

FuGENE® HD is a 100% synthetic, multi-component, non-liposomal transfection reagent designed for the delivery of DNA into more challenging eukaryotic and insect cell lines.  FuGENE® HD interacts with nucleic acids & the cell’s membrane to provide efficient and safe entry into the cell. This mechanism allows users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells, and has successfully been used to transfect over 1000 cell lines. With a protocol that is straight-forward and quick, FuGENE® HD allows researchers and scientists to free up valuable lab time.

Reference: OR01-500

Non-cytotoxic: Further experiments can be carried out with the same cells  Sensitive:  wide linear range with low to high numbers of cells detected  Easy to use: 15 minutes hands-on

Reference: EX04-20

Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.

Reference: C-12222

Human Coronary Artery Endothelial Cells (HCAEC)