Citrate buffer, 10X pH6.0 Reference: GTX30936 This buffer is intended for heat-induced epitope retrieval (HIER) of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC, commonly used fixatives (like formalin) mask tissue antigens through cross-linking, often resulting in poor or no staining. The use of this buffer on FFPE tissue sections improves antibody accessibility to tissue antigens.
EDTA buffer, 10X pH 8.0 Reference: GTX30937 This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.