FuGENE® HD Transfection Reagent Reference: HD-5000 FuGENE® HD is a 100% synthetic, multi-component, non-liposomal transfection reagent designed for the delivery of DNA into more challenging eukaryotic and insect cell lines. FuGENE® HD interacts with nucleic acids & the cell’s membrane to provide efficient and safe entry into the cell. This mechanism allows users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells, and has successfully been used to transfect over 1000 cell lines. With a protocol that is straight-forward and quick, FuGENE® HD allows researchers and scientists to free up valuable lab time.
Exo-spin midi columns Reference: EX04-20 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Trypsin-EDTA (0.05 %) in DPBS (1x) Reference: TRY-1B Trypsin solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.
DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate Reference: DMEM-LPXA Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
FuGENE® 6 Transfection Reagent Reference: F6-1000 FuGENE® 6 is a multi-component lipid-based transfection reagent that allows for the efficient delivery of all sizes of DNA into a broad range of cell types with extremely low cytotoxicity. FuGENE® 6 saves time and resources because it minimizes reagent preparation, media changes, and optimization. It also eliminates the side effects of toxicity while providing high transfection efficiency, which leads to successful transfection of more than 800 cell lines. FuGENE® 6 can be used for the study of gene expression, cancer biology, cell-based protein & virus production, and for many other applications in functional genomics, proteomics, and cellular analysis.
Exo-spin mini-HD columns Reference: EX05-5 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Trypsin-EDTA (0.5 %) in DPBS (10x) Reference: TRY-1B10 Trypsin solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.
Ham’s F-12, with L-Glutamine Reference: HAM-12-A Ham's F-12 was developed for the cultivation of Chinese Hamster Ovary (CHO) cells. It is the medium of choice for supporting the growth of cells in serum-free conditions.
FuGENE® 6 Transfection Reagent Reference: F6-5000 FuGENE® 6 is a multi-component lipid-based transfection reagent that allows for the efficient delivery of all sizes of DNA into a broad range of cell types with extremely low cytotoxicity. FuGENE® 6 saves time and resources because it minimizes reagent preparation, media changes, and optimization. It also eliminates the side effects of toxicity while providing high transfection efficiency, which leads to successful transfection of more than 800 cell lines. FuGENE® 6 can be used for the study of gene expression, cancer biology, cell-based protein & virus production, and for many other applications in functional genomics, proteomics, and cellular analysis.
Exo-spin mini-HD columns Reference: EX05-20 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.