RPMI 1640, powder medium, with L-Glutamine, w/o Sodium Bicarbonate Reference: RPMI-A-P50 RPMI 1640 medium is widely suitable for supporting growth of many cell types. Compared to other media, it has a unique formulation comprising glutathione and high concentrations of vitamins.
Exo-spin Buffer Reference: EX06-250 Exo-spin™ products provide a proven, rapid and reliable way of generating high-quality purified exosomes suitable for a range of research applications.
Exo-spin midi columns Reference: EX04-5 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Accutase Cell Detachment Solution Reference: ACC-1B Accutase Cell Detachment Solution is suitable for the routine detachment of cells. It is a gentle alternative to trypsin and effective on numerous cell types.
RPMI 1640, with L-Glutamine, powder medium, with 25 mM HEPES, w/o Sodium... Reference: RPMI-HA-P10 RPMI 1640 medium is widely suitable for supporting growth of many cell types. Compared to other media, it has a unique formulation comprising glutathione and high concentrations of vitamins.
Orangu™ Cell Counting Solution Reference: OR01-500 Non-cytotoxic: Further experiments can be carried out with the same cells Sensitive: wide linear range with low to high numbers of cells detected Easy to use: 15 minutes hands-on
Exo-spin midi columns Reference: EX04-20 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Trypsin-EDTA (0.05 %) in DPBS (1x) Reference: TRY-1B Trypsin solution is obtained from porcine pancreas and suitable for cell detachment and dissociation of various cell types. A variety of available formulations suit many applications.
RPMI 1640, with L-Glutamine, powder medium, with 25 mM HEPES, w/o Sodium... Reference: RPMI-HA-P50 RPMI 1640 medium is widely suitable for supporting growth of many cell types. Compared to other media, it has a unique formulation comprising glutathione and high concentrations of vitamins.
Orangu™ Cell Counting Solution Reference: OR01-1000 Non-cytotoxic: Further experiments can be carried out with the same cells Sensitive: wide linear range with low to high numbers of cells detected Easy to use: 15 minutes hands-on
Exo-spin mini-HD columns Reference: EX05-5 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.