Category: Cell Culture

Active filters

  • Categories: Aminoacids
  • Categories: Buffers
  • Categories: Cell Culture Media
  • Categories: Other Serum
  • Categories: Transfection
Reference: BRI10000-100mL

BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma production and for improving the efficiency of hybridoma cells cloning. BriClone completely replaces the need for feeder cells. The use of feeder cells to support the outgrowth of hybridomas has many disadvantages, like batch-to-batch variation of the feeder cells and competition between feeder cells and freshly-fused hybridomas for nutrients in the culture medium. BriClone has been specially developed to overcome these common problems in the post-fusion period.

Reference: BR-002-100mL

BriClone is produced by exposing basal culture medium with a low percentage of fetal calf serum to a human cancer cell line, which secretes relevant molecules into the medium, resulting in a product consistently supportive of the fusion and cloning stages of hybridoma production. Some researchers require a product which is serum-free and has had no contact with human or animal cells. We have, therefore, designed BriClone-SF which has the same basal medium and biological activities as BriClone but has no added serum or other substances of human or animal origin and has not been exposed to cells. BriClone-SF contains added recombinant human proteins produced in bacterial or yeast host cells.