Exo-spin blood Reference: EX02-50 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Hygromycin B, Solution (50 mg/ml) Reference: HYG-H Hygromycin B solution is a selection antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. Transformed cells containing the hygromycin resistance gene (hyg or hph) can be selected.
Phytohemagglutinin (PHA-M) Reference: PHA-K Phytohemagglutinin (PHA-M) is a mitogen used for the stimulation of cell proliferation in lymphocyte cell cultures. It also has a powerful erythroagglutinating property.
Exo-spin columns Reference: EX03-8 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Puromycin, Powder Reference: PUR-1X Puromycin is a selection antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. Transformed cells containing the puromycin N-acetyl-transferase gene (pac) can be selected.
FuGENE® 4K Transfection Reagent Reference: 4K-1000 FuGENE® 4K is our newest and most advanced, 100% synthetic, multi-component transfection reagent designed for the delivery of DNA into both challenging and routine mammalian cell lines. FuGENE® 4K was developed to improve upon our widely published and trusted FuGENE® HD reagent. Leveraging our gentle and highly efficient proprietary transfection chemistry, FuGENE® 4K provides efficient and safe entry of DNA into a wide range of cells. This mechanism allows users to obtain higher transfection efficiences than other competitor reagents. Whether it’s for difficult-to-transfect lines such as primary cells, stem cells, or suspension adapted 293 or CHO cells, our quick & easy FuGENE® 4K protocol allows users to obtain superior results and free-up valuable lab time.
Phytohemagglutinin M (PHA-M) for Lymphocyte Stimulation Reference: PHA-H Phytohemagglutinin M (PHA-M) for Lymphocyte Stimulation is a mitogen used for the stimulation of cell proliferation in lymphocyte cell cultures. It also has a powerful erythroagglutinating property.
Exo-spin columns Reference: EX03-25 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.