Exo-spin standard, 48 columns, 500 ml buffer Reference: EX01-50 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Colcemid Solution (10 µg/ml) in DPBS Reference: COL-H Colcemid Solution is commonly used in chromosome analysis during lymphocyte karyotyping. It arrests cells in metaphase so the chromosomes can be separated for further investigation.
DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium... Reference: DMEM-HPA-P50 Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
HEPES Buffer Solution (1 M) Reference: HEP-B HEPES Buffer Solution is a biological buffer used in cell culture media. Due to its stability, it is suitable for many different cell culture systems.
Exo-spin blood Reference: EX02-8 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium... Reference: DMEM-LPA-P10 Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Dulbecco’s PBS (10x), w/o Ca & Mg, w/o Phenol Red Reference: PBS-10XA Dulbecco's phosphate-buffered saline (DPBS) is a buffer solution used in numerous biological applications. It maintains the milieu in a viable physiological range to sustain cells.
Exo-spin blood Reference: EX02-25 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.