Exo-spin standard, 48 columns, 500 ml buffer Reference: EX01-50 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES Reference: DMEM-HHSTA Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Calf Serum Reference: CS-1A Calf Serum (Bovine Calf Serum, BCS) promotes growth of a variety of mammalian cell lines. It is often used as a cost-effective substitute for Fetal Bovine Serum.
Exo-spin blood Reference: EX02-8 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
DMEM High Glucose (4.5 g/l), with L-Glutamine, with Sodium Pyruvate Reference: DMEM-HPA Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Calf Serum Reference: CS-1B Calf Serum (Bovine Calf Serum, BCS) promotes growth of a variety of mammalian cell lines. It is often used as a cost-effective substitute for Fetal Bovine Serum.
Exo-spin blood Reference: EX02-25 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
HDMEC-c pre-screened Reference: C-12215 Human Dermal Microvascular Endothelial Cells (HDMEC) pre-screened
DMEM High Glucose (4.5 g/l), with Stable Glutamine Reference: DMEM-HPSTA Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Donor Horse Serum Reference: DHS-1A Donor Horse Serum is an alternative to bovine serum for growth factors and hormones in cell culture studies. It is broadly used as a blocking agent in immunoassays.