Exo-spin standard, 48 columns, 500 ml buffer Reference: EX01-50 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
G-418 Sulfate, Solution, 100 % Activity (50 mg/ml) Reference: G418-H G-418 Solution is used as a selection antibiotic in cell culture. It blocks polypeptide synthesis. Transformed cells containing the neomycin resistance genes neo can be selected.
Colcemid Solution (10 µg/ml) in DPBS Reference: COL-H Colcemid Solution is commonly used in chromosome analysis during lymphocyte karyotyping. It arrests cells in metaphase so the chromosomes can be separated for further investigation.
Exo-spin blood Reference: EX02-8 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
G-418 Sulfate, Powder Reference: G418-Q G-418 is used as a selection antibiotic in cell culture. It blocks polypeptide synthesis. Transformed cells containing the neomycin resistance genes neo can be selected.
Exo-spin blood Reference: EX02-25 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
G-418 Sulfate, Powder Reference: G418-R G-418 is used as a selection antibiotic in cell culture. It blocks polypeptide synthesis. Transformed cells containing the neomycin resistance genes neo can be selected.