HEK|ONE T, chemically defined medium for transient expression, w/o... Reference: HEKT-1000ML HEK|ONE T is a serum-free, chemically defined and animal-component-free medium for transient Human Embryonic Kidney 293 (HEK293) cells.
HEK|ONE S, chemically defined medium for stable expression, w/o L-Glutamine,... Reference: HEKS-1000ML HEK|ONE S is a serum-free, chemically defined, animal component-free and protein-free medium for stable Human Embryonic Kidney 293 (HEK293) cell lines.
HEK|ONE Feed, chemically defined feeding supplement, w/o L-Glutamine Reference: HEKF-1000ML HEK|ONE F is a serum-free, chemically defined feeding supplement for stable and transient HEK293 cells. It can be combined with HEK|ONE S or HEK|ONE T.
Blocking Buffer, TBS with BSA (10X) Reference: GTX48882 GeneTex's BSA Blocking Buffers are ready-to-use, 10X PBS or TBS solutions of bovine serum albumin protein for blocking steps in Western blotting, ELISA, immunohistochemistry and nucleic acid detection methods.These blocking buffers are 10% (w/v) solutions of high-quality BSA that are useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. Blocker BSA Buffers are most frequently diluted 10-fold (to 1% BSA) for initial testing, but other buffer concentrations can be beneficial for specific systems. BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid endogenous biotin.The purpose of the blocking step in an assay is to improve assay sensitivity by reducing background interference. However, unforeseen cross-reaction of detection reagents with blocking buffers is itself a cause of high background and low signal-to-noise ratios in assay systems. Because individual blocking buffers are not compatible with every system, a variety of blockers in both Tris-buffered saline (TBS) and phosphate-buffered saline (PBS) are available. The best blocking buffer for a specific experiment will bind to all potential sites of nonspecific interaction, eliminating background without altering or obscuring the epitope for antibody binding.To optimize the blocking step for a particular immunoassay, empirical testing is essential. Using inadequate amounts of blocker will result in excessive background. Using an excessive blocker concentration can mask antibody-antigen interactions or inhibit the marker enzyme. For best results when developing a new immunoassay, test several different blocking agents for the highest signal-to-noise ratio in the assay. There is no single blocking agent that is ideal for every occasion because many factors can influence nonspecific binding, including various protein interactions unique to a specific assay system.Features and Benefits: ‧ Purified protein – 10% solutions of high-quality bovine serum albumin; a single purified protein provides fewer chances of cross-reaction with assay components than serum or milk solutions ‧ Convenient – concentrated formulation saves storage space and can be diluted easily to obtain optimal blocking results for specific applications ‧ Easy to use – no waiting for powder to dissolve with this ready-to-dilute liquid concentrate ‧ Flexible – available in PBS and TBS formulations to suit a variety of applications
VeroVax, Serum-Free Medium, w/o L-Glutamine Reference: VEV-500ML VeroVax is a serum-free and animal/human origin-free medium that promotes the growth of Vero cells and the production of virus and recombinant proteins.