Category: Antibodies

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Reference: AT3004-mIgG1-100

Research Use Only Applications: Click Chemistry with TCO (trans-cyclooctene)-bound molecules. oYo-Link® Tetrazine allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two tetrazine labels per antibody for subsequent copper-free click chemistry reactions with TCO (trans-cyclooctene)-bound molecules. oYo-Link® Tetrazine limits the attachment of the tetrazine labels to the heavy chains of the antibody and therefore ensures that the tetrazine label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 tetrazine labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® Tetrazine with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3004-mIgG1-500

Research Use Only Applications: Click Chemistry with TCO (trans-cyclooctene)-bound molecules. oYo-Link® Tetrazine allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two tetrazine labels per antibody for subsequent copper-free click chemistry reactions with TCO (trans-cyclooctene)-bound molecules. oYo-Link® Tetrazine limits the attachment of the tetrazine labels to the heavy chains of the antibody and therefore ensures that the tetrazine label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 tetrazine labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® Tetrazine with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT4001-100

Research Use Only Applications: Conjugation of capture antibodies for Streptavidin-Coated Surface Immobilization in Direct ELISA; Also for Sandwich-ELISA oYo-Link® Single-Biotin is recommended for the immobilization of antibodies on Streptavidin-coated surfaces (or coated w/ other biotin-binding proteins). oYo-Link® Single-Biotin enables the site-specific labeling of oYo-Link®-compatible antibodies with a biotin to the IgG heavy chain. The use of a single biotin maximizes mobility and antigen binding density of surface-immobilized antibodies. Site-specific immobilization can lead to an improvement in sensitivity, stability and longevity of capture antibodies. While the magnitude of improvement is dependent on various parameters, such as antibody binding properties, uniform antibody orientation on a surface can result in an increase in sensitivity in enzyme-linked immunosorbent assays (ELISA) by enabling the capture of more antigens per unit area. The antibody labeling procedure simply requires mixing oYo-Link® Single-Biotin with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility email support@alphathera.com

Reference: AT4001-500

Research Use Only Applications: Conjugation of capture antibodies for Streptavidin-Coated Surface Immobilization in Direct ELISA; Also for Sandwich-ELISA oYo-Link® Single-Biotin is recommended for the immobilization of antibodies on Streptavidin-coated surfaces (or coated w/ other biotin-binding proteins). oYo-Link® Single-Biotin enables the site-specific labeling of oYo-Link®-compatible antibodies with a biotin to the IgG heavy chain. The use of a single biotin maximizes mobility and antigen binding density of surface-immobilized antibodies. Site-specific immobilization can lead to an improvement in sensitivity, stability and longevity of capture antibodies. While the magnitude of improvement is dependent on various parameters, such as antibody binding properties, uniform antibody orientation on a surface can result in an increase in sensitivity in enzyme-linked immunosorbent assays (ELISA) by enabling the capture of more antigens per unit area. The antibody labeling procedure simply requires mixing oYo-Link® Single-Biotin with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility email support@alphathera.com

Reference: AT4001-1000

Research Use Only Applications: Conjugation of capture antibodies for Streptavidin-Coated Surface Immobilization in Direct ELISA; Also for Sandwich-ELISA oYo-Link® Single-Biotin is recommended for the immobilization of antibodies on Streptavidin-coated surfaces (or coated w/ other biotin-binding proteins). oYo-Link® Single-Biotin enables the site-specific labeling of oYo-Link®-compatible antibodies with a biotin to the IgG heavy chain. The use of a single biotin maximizes mobility and antigen binding density of surface-immobilized antibodies. Site-specific immobilization can lead to an improvement in sensitivity, stability and longevity of capture antibodies. While the magnitude of improvement is dependent on various parameters, such as antibody binding properties, uniform antibody orientation on a surface can result in an increase in sensitivity in enzyme-linked immunosorbent assays (ELISA) by enabling the capture of more antigens per unit area. The antibody labeling procedure simply requires mixing oYo-Link® Single-Biotin with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility email support@alphathera.com

Reference: AT4001-mIgG1-100

Research Use Only Applications: Conjugation of capture antibodies for Streptavidin-Coated Surface Immobilization in Direct ELISA; Also for Sandwich-ELISA oYo-Link® Single-Biotin is recommended for the immobilization of antibodies on Streptavidin-coated surfaces (or coated w/ other biotin-binding proteins). oYo-Link® Single-Biotin enables the site-specific labeling of oYo-Link®-compatible antibodies with a biotin to the IgG heavy chain. The use of a single biotin maximizes mobility and antigen binding density of surface-immobilized antibodies. Site-specific immobilization can lead to an improvement in sensitivity, stability and longevity of capture antibodies. While the magnitude of improvement is dependent on various parameters, such as antibody binding properties, uniform antibody orientation on a surface can result in an increase in sensitivity in enzyme-linked immunosorbent assays (ELISA) by enabling the capture of more antigens per unit area. The antibody labeling procedure simply requires mixing oYo-Link® Single-Biotin with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility email support@alphathera.com

Reference: AT4001-mIgG1-500

Research Use Only Applications: Conjugation of capture antibodies for Streptavidin-Coated Surface Immobilization in Direct ELISA; Also for Sandwich-ELISA oYo-Link® Single-Biotin is recommended for the immobilization of antibodies on Streptavidin-coated surfaces (or coated w/ other biotin-binding proteins). oYo-Link® Single-Biotin enables the site-specific labeling of oYo-Link®-compatible antibodies with a biotin to the IgG heavy chain. The use of a single biotin maximizes mobility and antigen binding density of surface-immobilized antibodies. Site-specific immobilization can lead to an improvement in sensitivity, stability and longevity of capture antibodies. While the magnitude of improvement is dependent on various parameters, such as antibody binding properties, uniform antibody orientation on a surface can result in an increase in sensitivity in enzyme-linked immunosorbent assays (ELISA) by enabling the capture of more antigens per unit area. The antibody labeling procedure simply requires mixing oYo-Link® Single-Biotin with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility email support@alphathera.com

Reference: AT2002-100

Research Use Only Applications: Conjugation of capture antibodies with His-12 Tag for Transition Metal (e.g. Ni 2+, Co 2+) -Coated Surface Immobilization in Direct-ELISA; not recommended for Sandwich-ELISA. Also for Reactions with other His-reactive Groups oYo-Link® His-12 Tag is recommended for the immobilization of antibodies on Transition Metal (e.g. Ni ²⁺, Co ²⁺ ) coated surfaces (or coated w/ other His-binding proteins). oYo-Link® His-12 Tag enables the site-specific labeling of oYo-Link®-compatible antibodies with a His-12 tag to the IgG heavy chain. The use of a single His-12 Tag maximizes mobility and antigen binding density of surface-immobilized antibodies. Site-specific immobilization can lead to an improvement in sensitivity, stability and longevity of capture antibodies. While the magnitude of improvement is dependent on various parameters, such as antibody binding properties, uniform antibody orientation on a surface can lead to a > 10-fold increase in sensitivity in enzyme-linked immunosorbent assays (ELISA) by enabling the capture of more antigens per unit area. Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility email support@alphathera.com

Reference: AT2002-500

Research Use Only Applications: Conjugation of capture antibodies with His-12 Tag for Transition Metal (e.g. Ni 2+, Co 2+) -Coated Surface Immobilization in Direct-ELISA; not recommended for Sandwich-ELISA. Also for Reactions with other His-reactive Groups oYo-Link® His-12 Tag is recommended for the immobilization of antibodies on Transition Metal (e.g. Ni ²⁺, Co ²⁺ ) coated surfaces (or coated w/ other His-binding proteins). oYo-Link® His-12 Tag enables the site-specific labeling of oYo-Link®-compatible antibodies with a His-12 tag to the IgG heavy chain. The use of a single His-12 Tag maximizes mobility and antigen binding density of surface-immobilized antibodies. Site-specific immobilization can lead to an improvement in sensitivity, stability and longevity of capture antibodies. While the magnitude of improvement is dependent on various parameters, such as antibody binding properties, uniform antibody orientation on a surface can lead to a > 10-fold increase in sensitivity in enzyme-linked immunosorbent assays (ELISA) by enabling the capture of more antigens per unit area. Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility email support@alphathera.com

Reference: AT2002-1000

Research Use Only Applications: Conjugation of capture antibodies with His-12 Tag for Transition Metal (e.g. Ni 2+, Co 2+) -Coated Surface Immobilization in Direct-ELISA; not recommended for Sandwich-ELISA. Also for Reactions with other His-reactive Groups oYo-Link® His-12 Tag is recommended for the immobilization of antibodies on Transition Metal (e.g. Ni ²⁺, Co ²⁺ ) coated surfaces (or coated w/ other His-binding proteins). oYo-Link® His-12 Tag enables the site-specific labeling of oYo-Link®-compatible antibodies with a His-12 tag to the IgG heavy chain. The use of a single His-12 Tag maximizes mobility and antigen binding density of surface-immobilized antibodies. Site-specific immobilization can lead to an improvement in sensitivity, stability and longevity of capture antibodies. While the magnitude of improvement is dependent on various parameters, such as antibody binding properties, uniform antibody orientation on a surface can lead to a > 10-fold increase in sensitivity in enzyme-linked immunosorbent assays (ELISA) by enabling the capture of more antigens per unit area. Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility email support@alphathera.com

Reference: 60025

Detect proteins, protein-protein interactions, or protein modifications in cell samples. The assay is designed to be used with a mouse and rabbit primary antibody pair. Detection with TEX615 fluorophore is included.

Reference: 60017

Detect proteins, protein-protein interactions, or protein modifications in cell samples. The NaveniFlex Cell MR Atto647N assay is designed to be used with a mouse and rabbit primary antibody pair. Detection with Atto647N fluorophore is included.