Category: Antibodies

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Reference: AT3002-TAMRA

NOTE: It is important to perform the click-reaction with DBCO-x first, prior to antibody conjugation. UV illumination will damage the Azide activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Click Chemistry; Fluorescent imaging and/or quantification of oYo-Link® following conjugation or surface immobilization (e.g. to nanoparticles) oYo-Link® Azide allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two azide labels per antibody for subsequent click chemistry reactions. oYo-Link® Azide limits the attachment of the azide labels to the heavy chains of the antibody and therefore ensures that the azide label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 azide labels to be attached per antibody, providing precise antibody-conjugate ratios.The antibody labeling procedure simply requires mixing oYo-Link® Azide with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3003-100

NOTE: It is important to perform the click-reaction with Azide-x first, prior to antibody conjugation. UV illumination will damage the DBCO activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Copper-free Click Chemistry oYo-Link® DBCO allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two dibenzocyclooctyne (DBCO) labels per antibody for subsequent copper-free click chemistry reactions. oYo-Link®-DBCO limits the attachment of the DBCO labels to the heavy chains of the antibody and therefore ensures that the DBCO label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 DBCO labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® -DBCO with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list here or purchase AlphaThera's LED Photo-Crosslinking Device Here. Purchasing Information: For Credit Card purchases, proceed with checkout on website. To Request a Quote email sales@alphathera.com. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3003-500

NOTE: It is important to perform the click-reaction with Azide-x first, prior to antibody conjugation. UV illumination will damage the DBCO activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Copper-free Click Chemistry oYo-Link® DBCO allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two dibenzocyclooctyne (DBCO) labels per antibody for subsequent copper-free click chemistry reactions. oYo-Link®-DBCO limits the attachment of the DBCO labels to the heavy chains of the antibody and therefore ensures that the DBCO label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 DBCO labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® -DBCO with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list here or purchase AlphaThera's LED Photo-Crosslinking Device Here. Purchasing Information: For Credit Card purchases, proceed with checkout on website. To Request a Quote email sales@alphathera.com. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3003-1000

NOTE: It is important to perform the click-reaction with Azide-x first, prior to antibody conjugation. UV illumination will damage the DBCO activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Copper-free Click Chemistry oYo-Link® DBCO allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two dibenzocyclooctyne (DBCO) labels per antibody for subsequent copper-free click chemistry reactions. oYo-Link®-DBCO limits the attachment of the DBCO labels to the heavy chains of the antibody and therefore ensures that the DBCO label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 DBCO labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® -DBCO with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list here or purchase AlphaThera's LED Photo-Crosslinking Device Here. Purchasing Information: For Credit Card purchases, proceed with checkout on website. To Request a Quote email sales@alphathera.com. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3003-mIgG1-100

NOTE: It is important to perform the click-reaction with Azide-x first, prior to antibody conjugation. UV illumination will damage the DBCO activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Copper-free Click Chemistry oYo-Link® DBCO allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two dibenzocyclooctyne (DBCO) labels per antibody for subsequent copper-free click chemistry reactions. oYo-Link®-DBCO limits the attachment of the DBCO labels to the heavy chains of the antibody and therefore ensures that the DBCO label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 DBCO labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® -DBCO with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list here or purchase AlphaThera's LED Photo-Crosslinking Device Here. Purchasing Information: For Credit Card purchases, proceed with checkout on website. To Request a Quote email sales@alphathera.com. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3003-mIgG1-500

NOTE: It is important to perform the click-reaction with Azide-x first, prior to antibody conjugation. UV illumination will damage the DBCO activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Copper-free Click Chemistry oYo-Link® DBCO allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two dibenzocyclooctyne (DBCO) labels per antibody for subsequent copper-free click chemistry reactions. oYo-Link®-DBCO limits the attachment of the DBCO labels to the heavy chains of the antibody and therefore ensures that the DBCO label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 DBCO labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® -DBCO with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from the list here or purchase AlphaThera's LED Photo-Crosslinking Device Here. Purchasing Information: For Credit Card purchases, proceed with checkout on website. To Request a Quote email sales@alphathera.com. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3001-100

Research Use Only Applications: Reactions with Thiol-reactive Groups oYo-Link® Thiol enables the site-specific labeling of oYo-Link®-compatible antibodies with a Thiol to the IgG heavy chain. oYo-Link® Thiol limits the attachment of the thiol labels to the heavy chains of the antibody and therefore ensures that the thiol label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 Thiol labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® Thiol with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins. (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3001-500

Research Use Only Applications: Reactions with Thiol-reactive Groups oYo-Link® Thiol enables the site-specific labeling of oYo-Link®-compatible antibodies with a Thiol to the IgG heavy chain. oYo-Link® Thiol limits the attachment of the thiol labels to the heavy chains of the antibody and therefore ensures that the thiol label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 Thiol labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® Thiol with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins. (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3001-1000

Research Use Only Applications: Reactions with Thiol-reactive Groups oYo-Link® Thiol enables the site-specific labeling of oYo-Link®-compatible antibodies with a Thiol to the IgG heavy chain. oYo-Link® Thiol limits the attachment of the thiol labels to the heavy chains of the antibody and therefore ensures that the thiol label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 Thiol labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® Thiol with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins. (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3001-mIgG1-100

Research Use Only Applications: Reactions with Thiol-reactive Groups oYo-Link® Thiol enables the site-specific labeling of oYo-Link®-compatible antibodies with a Thiol to the IgG heavy chain. oYo-Link® Thiol limits the attachment of the thiol labels to the heavy chains of the antibody and therefore ensures that the thiol label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 Thiol labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® Thiol with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins. (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3001-mIgG1-500

Research Use Only Applications: Reactions with Thiol-reactive Groups oYo-Link® Thiol enables the site-specific labeling of oYo-Link®-compatible antibodies with a Thiol to the IgG heavy chain. oYo-Link® Thiol limits the attachment of the thiol labels to the heavy chains of the antibody and therefore ensures that the thiol label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 Thiol labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® Thiol with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins. (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AT3004-100

Research Use Only Applications: Click Chemistry with TCO (trans-cyclooctene)-bound molecules. oYo-Link® Tetrazine allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two tetrazine labels per antibody for subsequent copper-free click chemistry reactions with TCO (trans-cyclooctene)-bound molecules. oYo-Link® Tetrazine limits the attachment of the tetrazine labels to the heavy chains of the antibody and therefore ensures that the tetrazine label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 tetrazine labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link® Tetrazine with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com