25-OH Vitamin D3 Matched Antibody Pair Reference: REV-31-1315-MK The 25-OH Vitamin D3 Matched Antibody Pair is specific to 25-OH Vitamin D3 and has been demonstrated through ELISA. It does not cross-react with Vitamin D3, Vitamin D2, and 25-OH Vitamin D2. This kit includes capture and detection antibodies. 25-Hydroxyvitamin D3 is produced in the liver by 25-hydroxylation of cholecalciferol or Vitamin D3. It is a precursor for other Vitamin D3 metabolites and has only a limited biological activity in itself. Detection of 25-Hydroxyvitamin D3 is important for the diagnosis of Vitamin D deficiency, insufficiency or intoxication.
LAIR2 (human) Matched Antibody Pair Reference: REV-31-1371-MK LAIR-2 (CD306) is a secreted/soluble receptor, mainly produced by CD4+ T-lymphocytes, and is able to regulate the inhibitory potential of LAIR-1. Both LAIRs have been associated with several autoimmune diseases and inflammatory responses.
5-Methylcytosine (5-mC) DNA Matched Antibody Pair Reference: REV-31-1110-MK 5-Methylcytosine is a methylated form of the DNA base cytosine that regulates gene transcription and is involved in epigenetics. It is one of the best known epigenetic modifications. This methylation typically occurs at cytosine in CpG dinucleotides in vertebrates. When cytosine is methylated, the DNA maintains the same sequence, but the expression of methylated genes can be altered. 5-Methylcytosine is a very important repressor of transcription in the genome. When present in promoters, 5-Methylcytosine is associated with stable, long-term transcriptional silencing. This may occur by either blocking positive transcription factors, or promoting the binding of negative ones.
5-Hydroxymethylcytosine (5-hmC) DNA Matched Antibody Pair Reference: REV-31-1111-MK 5-Hydroxymethylcytosine (5hmC) is the first oxidative product in the active demethylation of 5-methylcytosine (5mC). It not only marks active demethylation but also serves as a relatively stable DNA mark that plays distinct epigenetic roles. It is potentially important in epigenetics, because the hydroxymethyl group on the cytosine can possibly switch a gene on and off. 5hmC is abundantly expressed, but the levels vary significantly depending on the cell type. The highest levels are found in neuronal cells of the central nervous system.
5-Carboxylcytosine (5-caC) DNA Matched Antibody Pair Reference: REV-31-1354-MK 5-Carboxylcytosine (5-caC) is a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.
5-Formylcytosines (5-fC) DNA Matched Antibody Pair Reference: REV-31-1369-MK 5-Formylcytosine (5fC) is a pyrimidine nitrogen base derived from cytosine and a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.