Category: Antibodies

Reference: AS10 1568

Reference: AT3002-100

NOTE: It is important to perform the click-reaction with DBCO-x first, prior to antibody conjugation. UV illumination will damage the Azide activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Click Chemistry oYo-Link® Azide allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two azide labels per antibody for subsequent click chemistry reactions. oYo-Link® Azide limits the attachment of the azide labels to the heavy chains of the antibody and therefore ensures that the azide label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 azide labels to be attached per antibody, providing precise antibody-conjugate ratios.The antibody labeling procedure simply requires mixing oYo-Link® Azide with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table) .. Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AS10 1569

Reference: AT3002-500

NOTE: It is important to perform the click-reaction with DBCO-x first, prior to antibody conjugation. UV illumination will damage the Azide activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Click Chemistry oYo-Link® Azide allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two azide labels per antibody for subsequent click chemistry reactions. oYo-Link® Azide limits the attachment of the azide labels to the heavy chains of the antibody and therefore ensures that the azide label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 azide labels to be attached per antibody, providing precise antibody-conjugate ratios.The antibody labeling procedure simply requires mixing oYo-Link® Azide with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table) .. Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AS10 1570

Reference: AT3002-1000

NOTE: It is important to perform the click-reaction with DBCO-x first, prior to antibody conjugation. UV illumination will damage the Azide activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Click Chemistry oYo-Link® Azide allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two azide labels per antibody for subsequent click chemistry reactions. oYo-Link® Azide limits the attachment of the azide labels to the heavy chains of the antibody and therefore ensures that the azide label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 azide labels to be attached per antibody, providing precise antibody-conjugate ratios.The antibody labeling procedure simply requires mixing oYo-Link® Azide with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AS10 687S

Concentration: after adding 90 µl of sterile milliQ water final concentration of the standard is 0.10 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Reference: AT3002-mIgG1-100

NOTE: It is important to perform the click-reaction with DBCO-x first, prior to antibody conjugation. UV illumination will damage the Azide activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Click Chemistry oYo-Link® Azide allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two azide labels per antibody for subsequent click chemistry reactions. oYo-Link® Azide limits the attachment of the azide labels to the heavy chains of the antibody and therefore ensures that the azide label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 azide labels to be attached per antibody, providing precise antibody-conjugate ratios.The antibody labeling procedure simply requires mixing oYo-Link® Azide with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AS11 1789S

Concentration: after adding 225 µl of sterile milliQ water final concentration of the standard is 0.1 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1 mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra careto mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing,

Reference: AT3002-mIgG1-500

NOTE: It is important to perform the click-reaction with DBCO-x first, prior to antibody conjugation. UV illumination will damage the Azide activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Click Chemistry oYo-Link® Azide allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two azide labels per antibody for subsequent click chemistry reactions. oYo-Link® Azide limits the attachment of the azide labels to the heavy chains of the antibody and therefore ensures that the azide label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 azide labels to be attached per antibody, providing precise antibody-conjugate ratios.The antibody labeling procedure simply requires mixing oYo-Link® Azide with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

Reference: AS15 2873S

Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Reference: AT3002-TAMRA

NOTE: It is important to perform the click-reaction with DBCO-x first, prior to antibody conjugation. UV illumination will damage the Azide activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Click Chemistry; Fluorescent imaging and/or quantification of oYo-Link® following conjugation or surface immobilization (e.g. to nanoparticles) oYo-Link® Azide allows for the site-specific labeling of oYo-Link®-compatible antibodies with up to two azide labels per antibody for subsequent click chemistry reactions. oYo-Link® Azide limits the attachment of the azide labels to the heavy chains of the antibody and therefore ensures that the azide label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 azide labels to be attached per antibody, providing precise antibody-conjugate ratios.The antibody labeling procedure simply requires mixing oYo-Link® Azide with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table). Requirements: To label your compatible antibodies with oYo-Link®, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com